Thesis (M.S.) in Biochemistry--University of Maine, 2002. / Includes vita. Includes bibliographical references (leaves 58-62).

Thesis (M.S. in environmental science)--Washington State University, December 2009. / Title from PDF title page (viewed on Feb. 10, 2010). "School of Earth and Environmental Science." Includes bibliographical references (p. 31-34).

Thesis (Ph. D.)--Rutgers University, 2010. / "Graduate Program in Biological sciences." Includes bibliographical references (p. 303-314).

Thesis (MSc)--University of Stellenbosch, 2007. / Bibliography. Also available via the Internet.

Cheung, Man Kit. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 81-93). / Abstracts in English and Chinese. / Abstract (English) --- p.I / Abstract (Chinese) --- p.III / Acknowledgements --- p.V / Table of contents --- p.VI / List of figures --- p.IX / List of tables --- p.XI / List of Appendices --- p.XII / Chapter Chapter 1. --- General introduction --- p.1 / Chapter 1.1. --- Picoeukaryotes --- p.1 / Chapter 1.2. --- Conventional characterization techniques --- p.1 / Chapter 1.3. --- Cloning and sequencing approach --- p.3 / Chapter 1.3.1. --- Applications in prokaryotic plankton --- p.3 / Chapter 1.3.2. --- Applications in eukaryotic picoplankton --- p.3 / Chapter 1.4. --- Variations in diversity with environmental factors --- p.5 / Chapter 1.5. --- Study site --- p.6 / Chapter 1.6. --- Objectives --- p.8 / Chapter Chapter 2. --- Materials and methods --- p.9 / Chapter 2.1. --- Study site --- p.9 / Chapter 2.2. --- Sample collection --- p.9 / Chapter 2.3. --- DNA extraction and 18S rRNA gene amplification --- p.11 / Chapter 2.4. --- Clone library construction and screening --- p.12 / Chapter 2.5. --- Sequencing and phylogenetic analysis --- p.13 / Chapter 2.6. --- Statistical analyses --- p.14 / Chapter Chapter 3. --- Results --- p.15 / Chapter 3.1. --- Hydrological parameters of study site --- p.15 / Chapter 3.2. --- Clone libraries --- p.15 / Chapter 3.3. --- Higher-level taxonomic distribution --- p.21 / Chapter 3.4. --- Phylogenetic affiliations of OTUs --- p.22 / Chapter 3.4.1 --- Alveolates --- p.35 / Chapter 3.4.2 --- Stramenopiles --- p.36 / Chapter 3.4.3 --- Rhizaria --- p.36 / Chapter 3.4.4 --- Other lineages --- p.37 / Chapter 3.4.5 --- Novel higher-level groups --- p.38 / Chapter 3.5. --- Diversity estimates of picoeukaryotes --- p.39 / Chapter Chapter 4. --- Discussion --- p.42 / Chapter 4.1. --- Picoeukaryotic diversity --- p.42 / Chapter 4.1.1 --- Overall diversity --- p.42 / Chapter 4.1.2 --- Diversity of individual taxonomic groups --- p.44 / Chapter 4.1.2.1 --- Most represented lineages --- p.44 / Chapter 4.1.2.2 --- Other photosynthetic lineages --- p.52 / Chapter 4.1.2.3 --- Other non-photosynthetic lineages --- p.55 / Chapter 4.1.2.4 --- Novel higher-level lineages --- p.56 / Chapter 4.2. --- Spatial and seasonal variations of picoeukaryotes --- p.58 / Chapter 4.2.1 --- Spatial variations --- p.59 / Chapter 4.2.1.1 --- Compositional variations --- p.60 / Chapter 4.2.1.2 --- Variations in diversity --- p.61 / Chapter 4.2.2 --- Seasonal variations --- p.65 / Chapter 4.2.2.1 --- Compositional variations --- p.65 / Chapter 4.2.2.2 --- Variations in diversity --- p.66 / Chapter 4.3. --- Methodological aspects --- p.67 / Chapter 4.3.1 --- Sample collection --- p.67 / Chapter 4.3.2 --- PCR amplification --- p.68 / Chapter 4.3.3 --- Cloning and RFLP screening --- p.69 / Chapter 4.3.4 --- Statistical estimates --- p.71 / Chapter 4.3.5 --- Future directions --- p.71 / Chapter Chapter 5. --- General conclusion --- p.73 / References --- p.81

The presented thesis includes three related projects, that are linked by a common interest in the evolution of eukaryotic organelles and machineries that import proteins into these compartments. The first project considers the possibility of peroxisomes (eukaryotic organelles known in aerobic organisms) being conserved in two related anaerobic protists: a free-living amoeba Mastigamoeba balamuthi and a parasite Entamoeba histolytica. The most important hint for the presence of peroxisomes was the discovery of proteins that are homologous to known components of the peroxisomal protein import machinery. The second project aims to characterize the unknown protein translocase of the inner membrane (TIM) in the mitosomes (extremely reduced mitochondria) of an anaerobic protozoan Giardia intestinalis. We have discovered an important subunit of the mitosomal translocase (Tim44), which usually tethers the Hsp70/PAM (presequence translocase-associated motor) complex to the TIM translocon. The last project shows that the protein translocase of the outer mitochondrial membrane in trypanosomatids is related to a typical eukaryotic channel Tom40. This finding is important because the absence of Tom40 was previously considered an ancestral feature of trypanosomatids.

Charles University in Prague, Faculty of Science Department of Parasitology Ph.D. study program: Parasitology Abstract of the Ph.D. Thesis Reductive Evolution of Mitochondria - Related Organelles in Anaerobic Protist Petr Rada Supervisor: Prof. RNDr. Jan Tachezy,Ph.D. Advisor: Doc. RNDr. Ivan Hrdý, Ph.D. Praha, 2011 1 ABSTRACT Trichomonas vaginalis and Giardia intestinalis are parasitic protists of the Excavata group. Both contain anaerobic forms of mitochondria called hydrogenosomes (Trichomonas) and mitosomes (Giardia). Hydrogenosomes produce hydrogen and ATP by substarte level phosphorylation and mitosomes represent the highly-reduced form of mitochondria that do not participate in cellular energy metabolism and ATP generation. Both types of organelles lost the majority of mitochondrial pathways and their genomes during the mitochondrion to hydrogenosome transition. Consequently, hydrogenosomes and mitosomes facilitate translocation of nuclearly encoded proteins into the matrix of the organelle as well as exchange of metabolites and ions across their membranes. Little is known about the membrane machineries required for the biogenesis of the organelle and metabolite exchange and the limited knowledge of mitosomal proteomes has been mostly gained from genomic analysis and localization studies of a few...

Thesis (M.S.)--Oregon State University, 2011. / Printout. Includes bibliographical references (leaves 71-79). Also available on the World Wide Web.

Thesis (MSc (Plant Biotechnology))--University of Stellenbosch, 2007. / The photosynthetic protist Euglena gracilis synthesizes a storage carbohydrate named paramylon, a glucan consisting only of β-(1-3)-glycosidic linkages. The enzyme that produces paramylon is a glycosyltransferase commonly known as paramylon synthase (EC 2.4.1.34; UDP-glucose: 1,3-β-D-glucan 3-β-D-glucosyl transferase). This enzyme uses UDP-glucose as its main substrate. In 2001, Bäumer et al. isolated and partially purified paramylon synthase, but never presented any sequence information. Hence, the main aim of this project was to isolate and characterize the gene(s) coding for the paramylon synthase. Different approaches were taken in order to isolate and characterize the gene(s). In the first part of the study molecular techniques were used to try and identify the gene. The two methods used were library screening and PCR amplification. Different libraries were screened using either functional staining or an affinity probe. The second method concentrated on the use of degenerate oligonucleotides, based on the amino acid sequences of conserved regions from known β-(1-3)-glucan synthase genes from various organisms, to PCR amplify the gene sequence from Euglena. These approaches were not successful in the isolation of the gene(s). In the second part of the study protein purification techniques were used in an attempt to obtain de novo protein sequence from the purified paramylon synthase enzyme. Several protein purification techniques were tried with the most successful being preparative ultra centrifugation followed either by sucrose density centrifugation or product entrapment (a type of affinity purification). These resulted in partial purification of the paramylon synthase protein. The partially purified proteins were separated using polyacrylamide gel electrophoresis, and the polypeptides able to bind the precursor, UDP-glucose, were identified using a radiolabeled isotope of UDP-glucose. These polypeptides were subjected to LC-MS-MS in order to obtain sequence information from them. One tryptic fragment showed high homology to β-(1,3)-glucan synthase genes from different yeasts.

The goal of this work was to examine the samples from archeological site in an attempt to identify human and/or animal intestinal parasite eggs. Another task was to detect parasitic protist antigens by ELISA test. Then the results were compared with other facts obtained from research of this locality.