Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

Ph.d.

Diss. (sammanfattning) Uppsala : Univ., 2003. / Härtill 5 uppsatser.

Thesis (M.S.)--University of Michigan, 1957.

Tritium NMR studies provide a convenient way of obtaining detailed information about conformational equilibria, dynamic processes and specific interactions in protein-ligand complexes provided that suitably 3H-labelled molecules are available. In this study [7,9-3H]- and [3',5',7-3H]folic acid, and [3',5',7-3H]methotrexate were synthesised and the NMR spectra of their complexes with Lactobacillus casei dihydrofolate reductase (DHFR) were assigned and analysed as a function of pH (DHFR-folate complexes) and temperature (DHFR-methotrexate complexes). From these data it was possible to obtain further evidence about the orientation of the pteridine ring in the complexes, and to monitor the dynamic processes in the bound ligands. In the 3H NMR spectra of the ternary complexes of the 3H-labelled folic acids with DHFR and NADP+, each labelled tritium gave rise to multiple signals, confirming previous findings that there are three interconverting, pH dependent, conformational forms of bound folate (forms I, IIa and IIb) in the ternary complex. The folate benzoyl ring could be shown to be in essentially the same environment in the different forms with the major differences being associated with the pterin ring. The appearance of a single resonance for the 3',5'-tritons showed that the benzoyl ring is flipping rapidly in all three forms. In contrast, the methotrexate binary complex and also the ternary complex with NADPH were shown to exist as a single conformational state with the benzoyl ring flipping rate being too slow to give a single averaged signal for the 3',5'-tritium nuclei over the temperature range 283 - 313 K. 3h{1h} Nuclear Overhauser enhancement experiments have been conducted on the small molecules, [3H]dimethyl sulphoxide, [3',5',7-3H]folic acid and [3',5',7-3H]methotrexate as a prelude to 3H-1H heteronuclear NOE experiments on binary and ternary complexes formed using Lactobacillus casei DHFR and the ligands [3',5',7-3H]methotrexate and [3',5',7-3H]folic acid.

No description available.

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [188]-197).

Thesis (M.S. - Hydrology and Water Resources)--University of Arizona, 1983. / Includes bibliographical references (leaves 98-101).

No description available.

An investigation was undertaken to compare airborne and ground-based radiometric survey techniques and to compare and contrast their relative merits and how they can be used in determining the environmental distribution of environmental radioisotopes, particularly those in the decay chains of 238U, 232Th and 40K. Before a detailed investigation was done of the survey techniques, it was necessary to look at the underlying physical principles of detection of ionizing radiation and the types of detector that are generally used in these different types of survey techniques. Having looked at the physics of detection, a detailed examination of the potential distribution of these environmental radioisotopes was undertaken. In these surveys, an assumption is often made, that the daughter isotopes in a decay chain are in secular equilibrium with one another. This assumption was examined and the various possible ways in which secular equilibrium could break down were considered, these included looking at biological, meteorological and chemical processes. Only after all the influences on assumptions used in these survey processes and the physical limitations on the measurements taken during surveys were considered, was a comparison made of a set of airborne and ground-based measurements taken at a chosen survey site compared. These measurements compared uranium, thorium and potassium activity concentrations, which had been determined by the two survey techniques, and a correlation was found particularly when the uranium measurements were examined.