A ratiometric laser induced fluorescence (LIF) thermometry applied to micro-scale temperature measurement for natural convection flows. To eliminate incident light non-uniformity and imperfection of recording device, two fluorescence dyes are used: one is temperature sensitive fluorescence dye (Rhodamine B) and another is relatively temperature insensitive fluorescence dye (Rhodamine 110). Accurate and elaborate calibration for intensity ratio verses temperature obtained using an isothermal cuvette, which was controlled by two thermo-bathes. 488nm Ar-ion laser used for incident light and two filter sets used for separating each fluorescence emission. Thermally stratified filed of 10mm channel with micro-scale resolution measured within 1.3?C uncertainty of liner prediction with 23?m x 23?m spatial resolution. Natural convection flows at 10mm channel also observed. The several difficulties for applying to heated evaporating meniscus were identified and a few resolutions were suggested.