Thesis (Ph. D.)--University of Texas at Austin, 2005. / Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Texas at Austin, 2005. / Vita. Includes bibliographical references.

Thesis (M.S.)--Texas State University-San Marcos, 2007. / Vita. Includes bibliographical references (leaves 68-75).

Thesis (M.S.)--Texas State University-San Marcos, 2006. / Vita. Appendix: leaves 31-35. Includes bibliographical references (leaves 36-39).

Thesis (Ph.D.) - University of Queensland, 2007. / Includes bibliography.

Thesis (M.S.)--Texas State University-San Marcos, 2006. / Vita. Appendix: leaves 31-35. Includes bibliographical references (leaves 36-39).

No description available.

Frog sartorius muscles were illuminated with laser light (λ = 457.9 - 632.8nm), stimulated electrically and stretched at the plateau of an isometric tetanus. An electro-optic system was used to rapidly (~7kHz) switch the electric vector of the incident beam through π/2 radians (between φ = 0° and 90°, relative to the muscle long axis) and 'simultaneous' recordings of transmitted light intensity (Ia) were made at orthogonal beam orientations during single contractions, using a sample-hold device. Stretch causes Ia to fall: this is represented either as a decrease of transparency or an increase of turbidity (τ). The amplitudes of the optical transients vary in direct proportion to the tension increment generated by stretch (which is related to the extent of actin-myosin filament overlap) and are highly anisotropic with respect to both λ and φ. At any given λ the amplitude for φ = 0° > φ - 90°. Both 0° and 90° signals vary inversely with λ: the wavelength exponent for the former is -2.39 and for the latter, -3.87. An attempt is made to analyse the changes in conservative dichroism (= Δτ0° - Δτ90°) using a model in which the scattering elements are forced to undergo a change of angular orientation (Δγ). The size of the scattering particles is estimated to be ~17nm (long axis) and to occupy ~0.038 of the fibre volume. It is postulated that the dichroic signal is due to a change in cross-bridge head (= S-1 HMM) orientation. The linear dimension of the actin-myosin interactive surface is estimated (from Δγ) to be >4.8nm. The results are interpreted in terms of a multi-step force generating cycle, based on the Huxley-Simmons model in which each head progresses through as many as 5 to 7 discrete positions during its working stroke, each separated from its neighbour by a potential difference energy of ~0.46x10-20J.

No description available.

No description available.