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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Assessment of DNA Profiling in Reconstructing the History of Natural Populations and Identifying Conservation Units / DNA Profiling and Population History in Conservation

Wilson, Paul January 2000 (has links)
The fundamental objective of conservation genetics is the identification of the basic units of conservation. Central to this objective is the reconstruction of the adaptive and evolutionary history of populations to evaluate their conservation status. Evolutionary history involves both microevolutionary and macroevolutionary processes and adaptive history is the evolution of specific characters to selective ecological processes in differential heterogeneous environments. Neutral DNA markers such as mitochondrial DNA, minisatellites and microsatellites are most often used for reconstructing history and identifying conservation units. This thesis examined three biological systems: 1) an African cichlid, 2) Canadian moose populations and 3) eastern North American wolves and coyotes to test two hypotheses. Firstly, neutral DNA markers can be used to accurately reconstruct the evolutionary history of populations. Secondly, neutral DNA markers are concordant with adaptive distinctiveness in reconstructing the adaptive history of populations. Few studies have examined these relationships. Lake Magadi tilapia showed discordant patterns between adaptive morphological, physiological and behavioural characters and genetic structure assessed with mitochondrial DNA. I propose this discordance has resulted from selection acting on mitochondrial DNA that has often been assumed to be "neutral". Neutral DNA markers accurately reflected the known history of the moose populations but discordant patterns were observed between neutral and functional loci indicating the former may not accurately reflect adaptive variation. DNA profiles of eastern wolves and coyotes showed a significant conflict in the interpretation of mtDNA and microsatellite data compared to previous genetic studies that examined wolf taxonomy. The data were consistent with the hypothesis of a North American-evolved wolf. Coyote-like mtDNA was not of coyote origin but represented divergent but related sequences of a North American wolf lineage independent of the gray wolf (C. lupus). Under this new model of eastern wolf evolution, we also identified the hybrid origin of eastern coyotes, contrary to previous interpretations, and genetically characterised different wolf "types" within Ontario. These findings could not reject the first hypothesis as neutral markers were used to reconstruct the histories of the three biological systems. However, the findings identified that it is important to ensure the neutrality of DNA markers and that samples are representative of the taxa under investigation. The findings in this thesis did not support the second hypothesis, as neutral DNA markers were not concordant with adaptive characters, i.e. morphology, physiology and functional genetic markers. / Thesis / Candidate in Philosophy
2

Telling History Through the Stories of Women: Julia Alvarez's In the Time of the Butterflies and In the Name of Salomé

Carlson, Nicole Marie 15 July 2006 (has links) (PDF)
My thesis discusses the ways in which Julia Alvarez's In the Time of the Butterflies (1994) and In the Name of Salomé (2000) are revolutionary texts contesting traditional, male dominated history and redirecting historical and communal foci to the lives of Dominican women. I employ Walter Benjamin's theories found in his essays "The Storyteller" (1936) and "On the Concept of History" (1940) to assist my exploration of Alvarez's questions concerning the power and effect of storytelling, and the importance of reconstructing various historical voices and images, specifically, the importance of reconstructing female voices in male dominated cultures. I discuss the female-narrated component to Dominican history which Alvarez creates in her reconstruction of the lives of these women. Alvarez confronts the challenge of breaking these women out of their marginalized status by combining fiction with history in her reconstruction of their lives. Alvarez assumes the multifaceted role of mediator, story-teller, and historian as she remembers and re-presents Dominican history through the eyes of women who lived, experienced, and affected change within the Dominican Republic. Without merely act as a reporter of historical "facts," Alvarez reconstructs the lives of these women fictionally, applying her impressions and ideas about the personalities, feelings, and thoughts of these women, and historically, utilizing first and secondhand accounts and information about the women. Ultimately, the women are presented as individuals but are also connected to a collective memory and history. As individuals with human characteristics, the women are no longer inaccessible legends. As members of a collective memory and history, the women are redeemed from the isolating effect of their patriarchal society which would have women remain silent. Due to Alvarez's reconstruction, their stories finally have the potential for further dissemination in the future with the possibility to affect other oppressed peoples. Thus, Alvarez's reconstruction of the resistance of a few women in Dominican history produces the capacity for additional resistance by Alvarez's audience to the same forces that these women were combating which continue to exist today — forces such as patriarchy, dictatorial governments, fascism, and economic disparity.

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