Thesis (Ph. D.)--Clark University. / Reprinted from American Journal of Psychology, vol. VI, no. 1. Bibliography: p. [46]-48. Also issued in print.

Stuttgart, Univ., Diss., 2007.

Thesis (Ph. D.)--Clark University. / Reprinted from American Journal of Psychology, vol. VI, no. 1. Bibliography: p. [46]-48.

This study analyzed the regenerative ability of cardiac muscle in the rat. Normal cardiac muscle from the rat was minced into one mm 3 fragments and homotransplanted into a gastrocnemius cavity of a non-sibling rat. At 45 days post surgery the regenerated tissue was removed and histological characteristics and oxidative capacity of the tissue were regenerate revealed the presence of myofibers. These myofibers of the cardiac regenerate typically exhibited centrally located, large, oval nuclei and branching. Both of these histological characteristics are typical of normal cardiac muscle. The absence of definitive intercalated discs though precluded conclusive identification of the myofibers of the cardiac regenerate as cardiac myofibers. The oxidative data showed that the cardiac regenerate consumed oxygen at a rate of 2.69X103 ± 2.96X102 (SE)y1 02/g X hr-1 this was found to be 4.9 times lower than normal cardiac tissue. The reason for this diminution is attributed to the observatiBall State UniversityMuncie, IN 47306

The fin exoskeleton of the zebrafish is comprised of lepidotrichia (or fin rays) and actinotrichia. In uncut fins, the fin rays span the entire length of the fin and the actinotrichia are found at the distal tips of each of the fin rays. Both of these fin exoskeletal components are capable of regenerating following amputation or injury. The regulation of the regeneration of these exoskeletal components is the central topic of this thesis and is explored in two different projects. The first project focuses on zebrafish fin ray regeneration during which bone segments are periodically added at the distal tips of each fin ray, each segment being separated by a joint. Joint formation involves the expression of a unique set of genes: hoxa13a, evx1, and pthlha. The alternation between bone segment formation and joint formation during fin ray regeneration seems to closely correlate with positional outgrowth during regeneration. We investigated whether or not the calcineurin and retinoic acid (RA) signalling pathways, both of which may be potential regulators of positional outgrowth, are involved in regulating joint formation. FK506-induced calcineurin inhibition and RA treatments each resulted in the suppression of joint marker expression. In RA-treated fins, bone deposition occurs in the joints as a result of joint cells being induced to differentiate into osteoblasts. These results suggest that the calcineurin and RA pathways may provide the positional information that regulates joint and bone segment formation. The second project focuses on the regulation of actinotrichia formation during adult fin regeneration. Throughout the early to intermediate stages of fin ray regeneration, actinotrichia fibers are found deep to the regenerating hemirays. As regeneration progresses, these actinotrichia fibers become gradually restricted to the distal domain of the fin regenerate. Actinotrichia contain structural proteins known as actinodin. There are four actinodin genes in zebrafish, actinodin1-4. We studied the comparative activity of the cis-acting regulatory elements of actinodin1 during fin regeneration. We have previously identified tissue-specific cis-acting regulatory elements in a 2kb genomic region upstream of the first exon, termed 2P, that drive reporter expression in the fin fold ectoderm and mesenchyme during embryonic development. Within 2P is a 150bp region, named epi, which contains an ectodermal/epithelial enhancer. Using in silico analysis, we have identified four main clusters of transcription factor binding sites within epi, termed epi1-4. Using a reporter transgenic approach, we have identified epi3 as a site containing an early mesenchymal-specific repressor and an epithelial-specific enhancer. We have also shown that the first exon and intron of actinodin1 contains a general transcriptional enhancer in adulthood and an alternative promoter. Overall, these results suggest that there is a difference between the regulation of actinodin1 during embryonic development and that of adult fin regeneration.

This study examines the social production of regenerated public places through the prism of the senses in two marginal city centre neighbourhoods: Castlefield in Manchester and el Raval in Barcelona. By combining ethnographic research with social theoretical frameworks it compares how a global process such as regeneration transforms the sensuous mapping of public places and how this refracts on the public life of the area. This `global ethnography' focuses on the processes and spatial practices embodied in both the production and daily use of these new public spaces, coupled with conceptions of `officials' and `users': planners, politicians, established residents, new residents, shop-owners, tourists, and so forth. The first part of the thesis theorises current developments in the spatial re-structuring of cities and analyses regeneration practices and conceptualisations. It is argued that new regeneratedp ublic spacesa nd emerging public lives must be assessedb y analysing their sensuously experienced `publicity'. A theoretical framework is developed, shaped by the concept of `socially embedded aesthetics', which examines how power relations in public places are constituted by, exercised through and embedded in the sensuous geography of place. In the second part of the thesis this conceptual framework is applied to both case studies. By analysing the social production of regenerated public spaces through Lefebvre's (1991) trialectics of the perceived, conceived and lived, a multi-layered analysis into the lived experience of regeneration by the different parties involved is provided. The thesis describes the effect that sensuous regimes have on exclusion and inclusion of particular social groups, meanings and practices in public space and life. It shows how sensuous transformations entail complex reinventions of public life, expressed through new spatial configurations and connected spatial contestations. Regeneration is portrayed as a process negotiated in daily practices and, while hegemonic forces aim at regulating public life, different user practices appropriate, divert or subvert imposed meanings through a variety of ways.

by Lao Jie. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (p. 168-182). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Tissue regeneration has intrigued biologists since the eighteenth century. While regeneration has been studied in many species, the cellular and molecular mechanisms governing successful compensation for lost body parts are poorly defined. This dissertation examines the cellular and molecular correlates of a form of regeneration defined as morphallaxis. Morphallaxis does not involve cell proliferation, but instead relies on the reorganization of existing tissues to recover body structure and function. Morphallaxis is a mechanism used during segmental regeneration (i.e., head or tail replacement) by the aquatic oligochaete, Lumbriculus variegatus. Here, morphallaxis of the nervous system is documented during segmental regeneration of Lumbriculus and during asexual reproduction. The morphallactic processes, which underlie changes in the neural anatomy and physiology of these worms, are reminiscent of mechanisms utilized by other neural plasticity events, including learning and memory. Proteomic and biochemical studies focus on a molecular marker of neural morphallaxis. The expression patterns of morphallaxis-associated-protein 66, MP66, are differentially regulated during both regeneration and asexual reproduction. This expression patterncorrelates with time-points of major cellular changes associated with neural morphallaxis. Thus, cellular and molecular events, demonstrated as part of neural morphallaxis in Lumbriculus, are recruited in two life-history contexts. Chemical disruption experiments, where either segmental regeneration or asexual fission are blocked, reveal that morphallaxis can be mechanistically dissociated from regeneration and reproduction. These results set a foundation for future investigations of specific mechanisms that mediate this novel form of neural plasticity.

No description available.

Thesis (Th. M.)--Talbot School of Theology, Biola University, 1990. / Includes bibliographical references (leaves 185-195).