Photocopy of typescript. / Digitized by Kansas Correctional Industries

Many mycorrhizosphere microorganisms can produce plant growth regulating compounds in vitro. Plant growth regulating compounds are known to mediate root growth and development. In reforestation practices, conifer tree seedling growth and survival are linked to rapid root growth and development after outplanting. Multifactorial experiments were conducted to document variations in conifer seedling growth, survival and endogenous root indole acetic acid (IAA) content as influenced by exogenously applied plant growth regulators and inoculation with ectomycorrhizal fungi capable of differential in vitro IAA and ethylene production. Capacity for in vitro IAA and ethylene production by mycorrhizal fungal isolates was partially correlated to levels of endogenous root IAA and root growth in inoculated seedlings as well as field transplanted nursery-grown seedlings. Although degree of correlation usually varied with fungal isolate as well as conifer species, IAA-mediated changes in root growth and seedling survival could indirectly be attributed to in vitro fungal plant growth regulator production capability. Exogenous application of plant growth regulating substances at different times during seedling growth and development resulted in significant changes in growth, survival and endogenous root IAA content of both experimental seedlings and field transplanted nursery-grown seedlings. Although effects varied with conifer species and stock types, IAA mediated increases in root growth and survival are projected to be cost beneficial. These results are not only indicative of the complexity of interactions which can occur between symbionts in the mycorrhizosphere but also suggest potential methods for manipulating plant growth regulator mediation of conifer root growth for practical purposes in reforestation. / Graduation date: 1994

by C.G. Aurell. / "June 27, 1947." / Bibliography: p. 20. / Army Signal Corps Contract No. W-36-039 sc-32037

Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2006. / Includes bibliographical references (leaves 70-74). Also available in electronic version.

Growth hormone binding protein (GHBP) may be an important factor in the regulation of growth as well as an indirect, less invasive way of predicting the status of growth hormone receptors. Several factors (age, nutritional status, sex, and glucocorticoid administration) have been reported to influence circulating growth hormone (GH) levels, growth hormone receptor (GHR) activity and/or GHBP in mammalian species. Therefore, the studies conducted in this research were designed to determine if these factors have any affect on serum GHBP in the young broiler chicken. Serum GHBP activity was expressed as a percent specifically bound ¹²⁵IhGH (%SB), as measured by a dextran-coated charcoal assay. Serum GHBP activity was highest (mean %SB= 14.6 ± 1.2) at hatch aniedecreased linearly (r= -.9516) to 4 wk of age (mean %SB= 4.1 ± 0.6). Sex had no significant affect on serum GHBP activity from hatch to 4 wk of age. Short term nutrient deprivation (24 h fast) of 4 wk old birds had no significant affect on serum GHBP activity, nor did refeeding. Feeding birds nutrient poor diets (low energy, low protein or low energy and low protein) did not significantly affect serum GHBP activity when compared to birds fed a commercial broiler diet. Pulsatile delivery of cortisone acetate (1, 5 and 10 mg/d/b) had no affect on serum GHBP activity at any dose. These results suggest that serum GHBP activity in the chicken is not affected by many factors which do influence GHBP in mammalian species. The lack of response to nutrient deprivation and cortisone acetate may be a factor related to the age of the birds used in these studies. / Graduation date: 1993

Protein-protein interactions regulate and drive biological processes and understanding the assembly of these interactions is important. The LysR-Type Transcriptional Regulators (LTTRs) are a large family of transcriptional regulators found in prokaryotes. I have used the LTTRs as a model for protein specificity. In order to understand a residue’s contribution to oligomerization, alanine-scanning mutagenesis was used to probe the contribution of residues identified from in silico analysis of two proteins: OxyR and CynR. The contribution of the residues to oligomerization was characterized using lcI repressor fusions. In OxyR, seven residues were identified as hot spots. Moreover, these hot spots are not especially conserved. The interaction surface of OxyR was mapped onto a multiple sequence alignment of the LTTR family. This mapping identified putative contacts in the CynR regulatory domain dimer interface. Combined with the in vivo testing, three residues were identified as hot spots. The residues identified in OxyR and CynR do not overlap. To investigate the assembly of the LTTRs I used a negative-dominance assay with lcI repressor fusions. Taken together, I show that the LTTRs in E. coli K-12 are mostly specific in their interactions.

Thesis (Ph.D.)--University of Nebraska-Lincoln, 2009. / Title from title screen (site viewed October 13, 2009). PDF text: xiii, 173 p. : col. ill. ; 6 Mb. UMI publication number: AAT 3355630. Includes bibliographical references. Also available in microfilm and microfiche formats.

Thesis (M.S.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains vi, 87 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 68-71).

Thesis (Ph.D.)--Tennessee Technological University, 2009. / Title from title page screen (viewed on Feb. 9, 2010). Includes bibliographical references.

Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 126-151).