Microalgae are promising candidates for biofuel production, CO2 biomitigation, and production of a variety of value-added products. However, high production costs and large energy consumption have been a major concern hindering the commercialization of microalgal products and processes. In addition, biological contamination and oxygen stress are two of the major contributors to these challenges. The objective of this project was twofold: 1) developing a novel strategy for control of biological contamination to enable non-sterile cultivation of microalgae such as N. oleoabundans, and 2) developing advanced deoxygenation mechanisms to reduce oxygen accumulation in the culture. It was found that addition of appropriate amount of NaHCO3 could effectively inhibit the growth of protozoa while its inhibition on microalgae was much less and could be alleviated by increasing pH to an appropriate level. It was also found that adding 160 mM NaHCO3 in media or decreasing incident light intensity to 100 W/m2 would help alleviate the oxidative stress to cells at 400% of air saturation. The feasibility of contamination-free non-sterile cultivation of freshwater green alga N. oleoabundans was verified using long-term continuous cultivation in a 15-liter TPBR with non-sterile medium and aeration. Furthermore, localized oxygen removal using hydrophobic hollow membranes was found to effectively reduce dO2 and increase lipid accumulation. These results have the potential to be translated into low-cost cultivation of freshwater microalgae processes for production of value-added microalgal products. At a more fundamental level, the mechanisms of the inhibition of NaHCO3 on microalgae and protozoa were discussed. Efforts were also made to simulate the effects of incident light intensity on light distribution, cell growth kinetics, and lipid accumulation of N. oleoabundans under non- sterile cultivation conditions.

Submitted by Jessica de Cássia Rossi null (jessicacrossi@yahoo.com.br) on 2016-10-05T23:00:49Z No. of bitstreams: 1 A desconstrução discursiva da mulher brasileira na mídia portuguesa - a intersecção dos marcadores da diferença.pdf: 1696987 bytes, checksum: 8a78aad835c9ab319af333880af627e0 (MD5) / Approved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2016-10-10T19:37:13Z (GMT) No. of bitstreams: 1 rossi_jc_dr_mar.pdf: 1696987 bytes, checksum: 8a78aad835c9ab319af333880af627e0 (MD5) / Made available in DSpace on 2016-10-10T19:37:13Z (GMT). No. of bitstreams: 1 rossi_jc_dr_mar.pdf: 1696987 bytes, checksum: 8a78aad835c9ab319af333880af627e0 (MD5) Previous issue date: 2016-08-01 / As experiências das mulheres brasileiras imigrantes têm merecido atenção da mídia portuguesa desde a chamada "segunda vaga" migratória, cujo marco temporal data de 1999. A feminilização dos fluxos migratórios confere centralidade às questões de gênero, as quais só podem ser entendidas em sua complexidade quando interseccionadas com outros marcadores sociais como nacionalidade, raça/etnia e classe. A fim de compreender como a mídia lusitana veio reportado este fenômeno, foram analisados nesta tese dois importantes periódicos locais, o lisboeta Público e Jornal de Notícias, do Porto. A partir da locução “mulher brasileira” e das palavras de busca “brasileira/s” chegou a um universo de 162 notícias, as quais foram organizadas em categorias classificatórias e analisadas em diálogo com os estudos feministas e pós-coloniais. Reiterações, cristalizações e tensionamentos relativos à experiência destas brasileiras imigrantes são problematizadas nesta pesquisa a partir de referencial foucaultiano. Considerando que os discursos são mais que atos de fala descritivos e sustentados por signos, procurou-se, justamente, sublinhar este “mais” no esforço de fazê-lo aparecer, procurou-se descrever seus sentidos. Os resultados mostram que os periódicos analisados tendem a reproduzir visões colonialista e sexistas relativas às experiências destas mulheres, ainda que, em um deles, voltado para o público lisboeta, de classe média, tenda a problematizar o preconceitos e estereótipos aos quais estas imigrantes estão sujeitas, a homogeneização das experiências, ali reportadas tende a reiterar percepções sexualizadas, erotizadas e/ou vitimarias das brasileiras. Invisibilizando-se vivências que não são facilmente alocadas nessas chaves de classificação. / The experiences of immigrants Brazilian women have come to the attention of the Portuguese media since the so-called "segunda vaga" immigration, which in timeframe date 1999. The feminization of migration flows gives centrality to gender issues, which can only be understood in its complexity when intersect with other social markers such as nationality, race / ethnicity and class. In order to understand how the media came Lusitanian reported this phenomenon, were analyzed in this thesis two major local newspapers, the Lisbon's Público and Jornal de Notícias, ot the Porto. From the expression "Brazilian woman" and the search words "Brazilian/s" reached a universe of 162 news, which were organized in classificatory categories and analyzed in dialogue with feminist studies and postcolonial. Reiterations, crystallization and tensions on the experience of these immigrants are Brazilian problematized this research from foucaultiano reference. Whereas the discourse are more than acts descriptive speech and sustained by signs, sought to precisely underline this "mais" in the effort to make it appear, he tried to describe his senses. The results show that the periodicals analyzed tend to reproduce colonialist and sexist views on the experiences of these women, although in one of them, facing the Lisbon audience, middle class, tend to question the prejudices and stereotypes to which these immigrants are subject the homogenization of experience, there tends to reiterate reported sexualized perceptions, eroticized and/or victimizers of Brazil. Unfeasible to experiences that are not easily allocated to this classification keys.

La croissance de caution vis-à-vis l'utilisation des produits pétroliers dans la construction a nécessité le besoin de développement des alternatives. Le BTP, en particulaire pour le secteur routière, a un certain nombre des piste ou les produits pétrolières ont utilisés, par exemple le liant pour les enrobés comme les agents régénérant (pour recyclage des enrobés), mais aussi le produits qui sont utilisés sur le chantier comme les anti-adhérents (ARA) et les débituminants (BR). Alors, il y a une besoin de remplacer les produits d'origines pétrolières par les alternatives bio-sourcés. Ce présent ouvre est une partie d'un projet qui va développer une nouvelle filière dédiée à la production de nouvelles formulations pour le BTP à partir de la transformation des huiles végétales et des graisses animales. Ce thèse concerne les produits développés seront destinés au l'asphalte, et alors, deux types d'applications sont particulièrement visés : (i) des agents anti-adhérents et (ii) des agents régénérants. Un débituminant sera développé aussi. Les produits anti-adhérents ont pour fonction d'empêcher le bitume d'adhérer aux engins de travaux sans effet secondaire, théoriquement, sur la matrice liante.. L'objet du travail est de caractériser l'efficacité et l'innocuité des agents anti-adhérents développés dans le cadre du projet et de développer les méthodes expérimentales visant à les évaluer. Trois essais principaux ont été développés pour caractériser la performance et innocuité des anti-adhérents vis-à-vis des essais qui ont optimisé leur implémentation et paramètres. La performance des anti-adhérents a été caractérisé par l'essai de glissance des enrobés. L'innocuité des anti-adhérents ont été caractérisés par des essais de fendage sur les enrobés et par l'essai de la dégradation de bitume. L'essai de la dégradation de bitume a servi aussi comme une mesure de la performance de débituminant. Les essais sur les anti-adhérents commerciaux d'Etats-Unis et la France a trouvé que ils ont deux modes de fonctionnement : i) lixiviation du bitume et ii) en formation d'une interface entre le les enrobés et la surface métallique. Même comme les deux modes ont été trouvé dans des certains anti-adhérents, le mode interface ont été trouvé préférable, grâce à l'option de garder les mêmes applications du produit pour plusieurs cycles de usage. Ensuite, un produit - basé sur glycérol sourcé de l'agro-industrie - a été développé. Les débituminants commerciaux ont été essayé, aves les conclusions : i) les débituminants plus effectifs ont eu les plus grande % des esters et ii) que les esters chaines courtes (C7-10) d'hautes concentrations ont été trouvé les plus efficace. L'agent régénérant est destiné à régénérer le bitume vieilli issu des agrégats d'enrobés (RAP) en lui redonnant ses propriétés originelles et en assurant leur maintien dans le temps. Le travail sur les agents régénérants se compose de la caractérisation physico-chimique du vieillissement du bitume et du bitume vieilli avec l'agent. Plus précisément, le travail se serve des techniques de spectrométrie IRTF (avec imagerie) et thermogravimétrie. Dans ce contexte, une produit bio-sourcé a été évalué comme une régénérant. Pour la spectroscopie FTIR, une moyenne de polir le mastique a été développé pour rassuré la platitude des échantillons. La régénération du bitume a été observé avec la spectrométrie ITRF, donc quelques peaks (notamment les indices IC=O and IS=O) pendant 0-42 jours de vieillissement dans l'étuve (loin-durée). Il a été trouvé qu'une période de vieillissement de 14 jours à l'étuve était à peu près équivalente au bitume vieilli par un cycle de RTFOT + PAV en termes de rhéologie et de la pénétration. Le mastic (vieilli 14 jours) a ensuite été mélangé avec un agent de bio-source (à 7,5% en poids de mastic). Il a été constaté par imagerie FTIR que l'indicateur de l'oxydation IS=O a été réduit par l'incorporation de l'agent régénérant dans le bitume. / The growing health and environmental concerns brought on by the use of petroleum based products in the asphalt construction industry have necessitated the development of alternatives. Infrastructure, especially that involving transportation has many uses for petroleum products including, as fuel, as well as in asphalt pavement construction - where petroleum products have traditionally constituted the binder for the mix as well as the rejuvenating agents (for asphalt recycling) - along with various agents used in the construction process including bitumen removers and asphalt release agents. Thus, there is a need to replace petroleum base agents with bio-sourced and biodegradable substitutes. The present work is part of a project to develop bio-sourced (recycled from agricultural waste) products for the construction industry. This work is dedicated to developing products relating to the asphalt industry. Two types of product applications were envisioned: i) an asphalt release agent (ARA) and ii) an asphalt rejuvenating agent. Additionally, a bitumen remover (BR) developed as part of the work on the ARA. ARAs prevent asphalt from adhering to tools and equipment used in asphalt production, without producing overly negative side effects with regards to the pavement. Three principal tests methods were developed and optimized for the performance and damage to asphalt of the ARAs. The asphalt slide test was developed to quantify the performance of the ARA by sliding hot asphalt mix down a plate with the ARA applied. The testing of the damage to asphalt from ARAs consisted of testing an asphalt cylinder - in contact with an ARA for seven days - in indirect-tensile strength (ITS). The bitumen degradation test consisted of submerging a bitumen sample in an agent over a certain time and weighing the bitumen that did not dissolve in the agent. This was followed by the observation of the bitumen-ARA chemical interaction by FTIR spectrometry. This test served as an assessment of ARA damage to bitumen as well as of the performance of BRs. The testing of the commercial ARAs from both the French and USA markets found that they had two primary modes of functioning: i) by softening the bitumen and ii) by forming an interface between the asphalt and the metal surface. While some agents had elements of both, it was found that interface agents are preferable, due to the ability to use a single ARA application for multiple occasions. With this completed, a water-based bio-sourced substrate ARA - based on glycerol derived from agricultural waste - was developed. The commercially available BRs were tested as well, finding that i) the most effective BRs had the highest ester concentration and ii) that highly concentrated short chained ester (C7-10) were very effective bitumen dissolvers. The goal of rejuvenating agents is to regenerate the old bitumen from recycled asphalt pavement (RAP) by restoring the original properties and ensuring the stability of these properties over time. This part consisted of the development of methods for bitumen and aging, as well as characterization of the chemical rejuvenation of bitumen by FTIR spectrometry (including imaging) and thermogravimetric analysis. A bio-sourced rejuvenating agent was evaluated as well. For the imaging, a mastic polishing method was developed in order to attain as samples as flat as possible for the analysis. The bitumen rejuvenation was observed using FTIR spectroscopy analysis. Several peaks (notably IC=O and IS=O) were observed for bitumen oven aged up to 42 days (long term). It was found that an oven aging period of 14 days was roughly equivalent to bitumen aged by a RTFOT+PAV cycle it terms of rheology and penetration. The mastic (aged for 14 days) was then combined with a bio-sourced agent (at 7.5%w of mastic). It was found by FTIR imaging that the oxidation indicator IS=O, was reduced by the incorporation of the rejuvenating agent.