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Loss of the ssp1 protein kinase (CAMKK) gene results in stress sensitivity, cell elongation, slow growth and in some cases cell cycle arrest. In order to identify new components of the ssp1 stress response pathway, a transposon mediated suppressor screen was used to identify loss of function suppressors of a Schizosaccharomyces pombe ssp1 gene disruption. The Musca domestica Hermes transposon was used to randomly insert the KanMx6 selectable marker in the genome. The selection was for Hermes insertions which rescued the G2 cell cycle arrest phenotype of ssp1- when grown at pH 3.5 and 36 C. Second site mutations that rescued the cell cycle arrest and allowed for colony formation were identified. In total 121 mutant strains with elongated morphology but capable of colony formation at pH 3.5 and 36 C were isolated and 22 insertion sites were identified by inverse PCR and sequencing. Genes for a transcriptional suppressor, scr1 (SPBC1D7.02c ), a spermidine transporter (SPCC569.05c ), cyp9 cyclophilin 9 (SPCC553.04), complexed with cdc5 (cwf4) (SPBC31F10.11c), ptr8 (SPAC17A5.06), (SPBC1921.07c), and set7 (SPCC297.04c) were identified as second site loss of function suppressors of the ssp1 deletion. Identifying these genes and their phenotype in conjunction with loss of ssp1, substantially improves our understanding of the Ssp1 molecular pathway in cell cycle control and cell stress response / Thesis (Master, Biology) -- Queen's University, 2014-01-27 15:55:29.75

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TAR1 is a protein coding gene situated antisense to the 25S rRNA in S. cerevisiae. Tar1p is localized to the mitochondrial inner membrane, and expression is enhanced under conditions of respiratory dysfunction. One common cause of respiratory dysfunction in S. cerevisiae are selfish mitochondrial mutants known as ρ- mitotypes. ρ- mitotypes exhibit drive within the cell following sexual reproduction ; outcompeting host cells and inducing respiratory dysfunction. Respiratory dysfunction activates the Retrograde Response, which involves the expression of genes to compensate for loss of anabolic activity that accompanies respiratory dysfunction. The Retrograde Response also leads to the formation of lifespan shortening Extrachromosomal rDNA circles. Amplification of rDNA circles has the effect of increasing TAR1 at the same time as lifting transcription repression. This observation led to the hypothesis that the formation of rDNA circles was a positive effect of the Retrograde Response, and that TAR1 may serve to ameoleriate the spread of respiratory incompetent mitochondria following sexual reproduction. In this thesis, experiments are conducted that show that TAR1 does suppress the drive of selfish mitochondrial mutants. Additional bioinformatic analyses show that the Retrograde Response may be a recent adaption to selfish mitochondrial mutants.

The Functional Limitation Profile (FLP), the Hospital Anxiety and Depression Scale (HADS) and the Recovery Locus of Control scale (RLOC) are three well established and useful measures used in Health Psychology. However, the reliable and valid measurement of these health predictors and outcomes has associated problems. The present thesis tackles measurement issues in all three instruments using item response theory (IRT). The Scientific Advisory Committee of the Medical Outcomes Trust has suggested the methodological and theoretical rationale for the conceptual and measurement model of available measurement instruments should be reported. The introduction chapter provides theoretical background in order to understand activity limitations and participation restrictions as behaviours affected by a certain health condition, as well as by thoughts and feelings. Within this theoretical framework, the present thesis investigates the measurement of mood using the HADS and functional limitations using the FLP in three different health conditions: (1) stroke patients, (2) patients with myocardial infarction, and (3) patients who underwent joint replacement surgery. The measurement of perceived personal control beliefs using the RLOC scale, and the relationship between control cognitions, mood and functional limitations were examined in stroke patients since all three measures were available for secondary analysis in this sample. The main findings are that (1) highly sensitive FLP items measure precisely different levels of disability and handicap, (2) removing 2 HADS items results in precise measurements of different levels of anxiety and depression, and (3) internal but not external perceived personal control beliefs measured sensitively different levels of the underlying construct.