Objectives Although the lack of perceived safety is an important deterrent to cycling, available knowledge is not comprehensive enough. The aim of this research is to contribute to academic knowledge by exploring the role of road section attributes in perceived safety of cyclists and to develop a method to use the theoretical results in practice. Methods A stated choice survey is carried out to estimate the effects of selected infrastructure attributes on perceived safety. A multinomial logit (MNL) model is used to estimate the effects. Results are used to develop an infrastructure assessment tool by counting aggregate perceived safety utility values of road sections. Results Cyclists perceive the presence of dedicated cycling facilities and physical separation similarly important, while other attributes (traffic volume, speed reduction and adjacent car parking) turned out to be less important. The Subjective Safety Score can be consciously used to evaluate existing and planned road sections and compare different design alternatives. Conclusions The results give a strong support for using physically separated cycling facilities (cycle tracks for example) to engage people to cycling. Further research is recommended to explore the effects of intersection attributes and to include interaction effects of attributes as well.