The objectives of this research were to study: 1) the effects of five different propagation media containing composted materials on the rooting of semi hardwood blueberry cuttings and 2) the effects of four cultivars and one genotype on rooting responses of these cuttings. The media used were: peat moss and horticulture grade per lite as control, composted pine bark fines with only one-time application of ammonium nitrate, pine bark fines with composted cotton gin waste, hardwood with and composted chicken litter and pine bark with composted chicken litter. There were no significant differences among the media for rooting from semi hardwood cuttings. Cultivars or genotype, media, and the interaction of individual media and cultivar or genotype had effect on rooting responses. Climax and Tifblue showed higher number of rooting from cuttings compared to other lines. Climax had the highest number of callus and survival percentage among all.