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This item was digitized as part of the Million Books Project led by Carnegie Mellon University and supported by grants from the National Science Foundation (NSF). Cornell University coordinated the participation of land-grant and agricultural libraries in providing historical agricultural information for the digitization project; the University of Arizona Libraries, the College of Agriculture and Life Sciences, and the Office of Arid Lands Studies collaborated in the selection and provision of material for the digitization project.

Nodal sections of the winter-hardy 'John Franklin' rose cultivar from field-grown plants were cultured on a modified Murashige and Skoog (MS) nutrient medium. Very high levels of contamination from the surface of the initial sections required that plants be grown under greenhouse conditions. Rose plantlets obtained from subsequent subcultures were used for the first time in a radiotracer experiment with $ sp{32}$P to study the kinetics of phosphorus (P) uptake as a function of temperature of the nutrient medium. P uptake increased with time for rooted and non-rooted plantlets in a linear fashion that did not reach an equilibrium value even after 96 hours of exposure. An analysis of variance revealed that the plantlets with roots absorbed significantly greater amounts of P at the 0.01 level compared with non-rooted plantlets at 22$ sp circ$C. P uptake was significantly higher at the 0.05 level, for rooted versus non-rooted plantlets at 33$ sp circ$C. There was no significant difference in P uptake by rooted and non-rooted plantlets at 3$ sp circ$C. Interaction between time of exposures and rooting was found to be significant at 22$ sp circ$C and 33$ sp circ$C at the 0.01 level. The results indicated that the root system, previously thought to be inefficient in the nutrient absorption, played a key role in absorbing P from the nutrient medium at optimum temperature.

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This study is an examination of landed society in the/ar North-West of England between the outbreak of Edward Ill's wars with Scotland in 1332 and the end of the first stage of the Wars of the Roses in 1461. Although violence within regional society both in terms of involvement in Anglo-Scottish relations of the period and domestic violence in the form of gentry feuds and - at a larger scale - magnate feuds during the Wars of the Roses, constitutes a major part of this thesis, rather more peaceful concepts are also explored. Firstly, it is necessary to define the extent of the region as a whole, debating whether there are any boundaries more meaningful than those political and administrative boundaries provided by county units; this is followed by a prosopographical reconstruction of the composition of landed society: the significant peerage and greater gentry families. It will be argued that in the far North-West the topographical patterns created by physical geography are of far greater significance than shire units for the greater gentry families of local landed society. This point is demonstrated by an analysis of gentry identity in terms of attendance at the county court, and - more importantly - in property and marriage settlements, which indicate the importance of sub-county units, especially in the small 'mini-county' of Lancashire North of the Sands (the Furness and Cartmel peninsulas). Examination of the construction and composition of magnate retinues and affinities - the Lucy, Percy, Neville, Clifford and Lancastrian affinities in particular - also suggests a similar conclusion. The theme of the final two chapters - Anglo-Scottish relations - tackles the supra-county level, in terms of how far south the Border mattered in the far North- West and considers the cultural and architectural phenomenon ofpele towers in the region. At both sub-county and supra-county level, the importance of physical geography over the 'longue duree' is very clear indeed.

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The culture in vitro was investigated in 7 clones of roses representing a range of genotypes and ploidy levels. Particular attention was paid to a sterile hybrid, R. persica x xanthina , from which it was hoped to obtain tetraploid clones. It was anticipated that tetraploid clones might be fertile and that this would facilitate introgressive hybridization of R.persica genes into various classes of cultivated roses. Propagation medium was developed based on MS salts and vitamins supplemented with BAP and NAA. Doubling times of 2-4 weeks were obtained on optimum media. Transplantation to soil was achieved with R.wichuraiana had a 99% success rate _____ conditions when a misting system was used whereas transplantation with R.ruRpsa 'Scabrosa' was only 46% successful and no success was achieved with R.persica x xanthina . Success rates were subsequently improved R.persica varying degrees of success, on transfer to in vivo to 80% with 'Scabrosa' and Sorbarod plugs prior to transplantation. Whilst in culture adventitious shoots were induced clones (R.laevigata , R.wichuraiana and R.persica R.persica shoots x xanthina by the use of from leaves of 3 x xanthina ) but only with adventitious callus. The cell established _____ procedure. This was exposure to colchicine gave x xanthina from _____ did it prove possible to induce internodal stem segments, roots, leaves and the cycle time of in vitro as 10.25 hours used in discussing diploid rose R.wichuraiana was using an autoradiographic the optimal duration of solution. It was complete spindle inhibition without a found that reduction 0.05% colchicine mitotic index. in The addition of DMSO was shown to aid the uptake of colchicine into shoot meristems. Terminal buds of R.wichuraiana and R.persica x xanthina were exposed level to colchicine solution in plants derived from these buds was and measuring length of stomata vitro and the determined by ploidy of karyotyping of root cells (LIII layer) (LI layer). X-irradiation was used in vitro to obtain different morphological forms, dose rates of 3, 4 and 5 krads producing 68, 100 and 80% mutant forms respectively. It is suggested that the combined use of drug and X-ray treatments, use of GA^ and protoplast fusion in vitro would be appropriate subjects for further investigation.

Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1992. / Includes bibliographical references (leaves 170-189).

Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1996. / Includes bibliographical references (leaves 221-240).

No description available.