Typescript (photocopy). / Digitized by Kansas Correctional Industries

LD2668 .T4 1954 S36 / Master of Science

Call number: LD2668 .T4 1956 H64 / Master of Science

At cable winding today one uses one portal, one caterpillar, one spooling boom and one tower and the same kind of set of equipment for unwinding. This kind of solution is both expensive and unpractical when one of the components of unwinding/winding is stationary for a long time without being used. To save unnecessary expenditure and maintenance it would have been practical to only use one set of caterpillar, tower, spooling boom and portal that later on can be rotated around the turntable when exporting the cable. The project that this work includes is to bring a concept that excludes two sets and thus provides the system with a rotatable spooling system. To get as much information about the product and of the manufacturing as possible several interviews has been done of the forging shop Ronneby Svets & Smide and also their fitters. At the concept generation several proposals was developed and to go further with only one of them a QFD and a SWOT analysis was made to get the best concept. Verification of solid mechanics on the construction with FEM-analysis has been implemented on beams, tubes and tube constructions. The project comprises constructing of details and drawings for structure of the concept and also commitment of assembly, maintenance, ergonomics and shipping. At the assembly has bolted joints been used on most attachments for fast assembling on site instead of welds. For commitment of maintenance it is thought that one should easily access for control and tighten afterwards of example the slewing ring. At the ergonomics the idea is put for example to accessing the workspace or maintenance areas in form of a ladder or a staircase. The shipping has played a major role for the construction parts and has then been divided for easiest and most practical way to be transported smoothly.

Background: Strength and power are important factors in many sports, and rotational power of the trunk and upper body are important components of golf performance. Trunk and pelvis cooperated in the rotation movement and strength in the muscles surrounding these segments is contributing to a successful and powerful rotation. Side-to-side asymmetry is often discussed in the field of injury prevention and it is suggested that bilateral imbalances affects the performance. Hand strength is a measure of diverse use when testing physical requirements in athletes. It has previously been concluded that hands strength correlates strongly with strength in both lower and upper extremity in youths, but the correlation among adults and athletes are unknown. In golf, hand strength has been stated to be one of the most important components for golf performance and it strongly correlates with golf specific variables such as ball speed and drivers distance. The relationship between hand strength and rotational power is unknown. Aim: The aim was to study rotational peak power and hand strength in young golf players to compare side-to-side differences and the relationship between rotational peak power and hand strength. Methods: Twenty-seven subjects, 22 men and 5 women, with a golf handicap of ≤5 participated in the study. The two tests performed was a hand strength test using a T.K.K handheld dynamometer and a rotational peak power test in Quantum. The tests were performed at the same session, first hand strength and then rotational peak power. In order to test the rotational peak power a 1RM test was performed. In order to study the relationship between hand strength and rotational power a Spearman´s range of correlation (rs) was used. A Wilcoxon signed-ranked test was used to study the side-to-side differences between dominant and non-dominant hand strength and rotational power. Results: No statistical significant differences were found between dominant and non-dominant hand strength (p = 0.28) or between dominant and non-dominant rotational power (p = 0.97). A strong correlation between dominant hand strength and dominant rotational power was found (rs = 0.636) and a moderate correlation between non-dominant hand strength and non-dominant rotational power (rs = 0.589). Conclusion: There are no side-to-side differences in hand strength or rotational power in young healthy golf players. Dominant hand strength correlates strongly with dominant rotational power.

Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, cloning mammalian species through somatic cell nuclear transfer has been simply inefficient in all species in which live clones have been produced, such as ‘Dolly’ the sheep, and ‘Samrupa’ the buffalo. Most of the experiments resulted failure, and the success rate ranges from 0.1% to 3%. Developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. Researchers have tried strategies to improve the efficiency of nuclear transfer. However, significant breakthroughs are yet to happen. The enucleation procedure consisting of extracting reprogrammable genetic material during nuclear transfer has been linked to inefficiencies due to manual error, lack of repeatability and decreased high throughput. Conventional manual enucleation process requires a series of complicated cell rotation in three-dimensional (3D) spaces using a blunt or sharp tipped pipette, and can puncture the cell during genetic material extraction. Current methods frequently damage the cell via physical or chemical contact, and thus have low throughput. Therefore, there is a need for simple, readily automated, non-contact methods for controlled cell rotation. Precise rotation of the suspended cells is one of the many fundamental manipulations in a wide range of biotechnological applications, such as cell injection and enucleation. Noticeably scarce from the existing rotation techniques is 3D rotation of cells on one single chip. To bridge this gap, this research presents a means of controlled cell rotation for bovine oocytes around both the in-plane (yaw) and out-of-plane (pitch) axes using a simple, low cost biochip fabricated using a mixture of conventional lithography and low-cost micro-milling. It uses a phase varying dielectrophoresis (DEP)-based electrorotation (EROT) biochip platform, which has an open-top sub-millimetre square chamber enclosed by four sidewall electrodes and two bottom electrodes to induce torque to rotate the cells about two axes, thus 3D cell rotation for the first time. Before fabrication, phase varying DEP-based rotational electric field simulations were carried out in the designed rotation chamber. For this analysis, initial rotational fields are characterised for both in-plane and out-of-plane axes using multi-physics finite element software. Electrode shape and chamber design were optimised using realistic parameters for the medium and electrode material properties. Results showed remarkable promise to rotate dielectric particles in rotational field strengths of the order of 104 V/m. From simulations, a basic biochip design was optimised. Within the fabricated biochip, controlled rotations around the in-plane and out-of-plane axes were demonstrated, and the electric field activation frequency range and electrokinetic properties of the bovine oocytes were characterised. Rotation was measured via video image processing with data included on electronic annex. Results show controllable rotation in steps of 5 degrees around both axes with the same chip. In experiments, the maximum rotation rate reached 150°/s in yaw axis and 45-50°/s during pitch axis, while a smooth, stable and controllable rotation rate was found below 30-40°/s. Optimum rotation rates are found for inputs of 10 Vp-p at 500-800 kHz AC frequency for yaw-axis rotation, and 10-20 Vp-p and 10-100 kHz for pitch-axis rotation. In addition, zona intact and zona free oocytes are shown to have electrical equivalence and found no noticeable difference, generalising the bio-chips capability and results. Further, experimental results were used to validate the numerical solid shell model used in design and it was found that the bovine oocytes are highly polarizable than the surrounding medium. Finally, the dielectric properties of the oocytes were fully characterised enabling further design optimization in future, if desired. The biochip was successfully designed, optimised and experimentally validated, and successful rotation of bovine oocytes in 3D spaces was demonstrated. These results create a platform tool for biologists to utilise enucleation with high throughput efficiency and ease. In summary, this simple, transparent, low-cost, open-top, and biocompatible biochip platform, allows further function modules to be integrated and is the foundation for more powerful cell manipulation systems. In brief key novel aspects of the research were: • Rotation of suspended spherical oocytes in multiple axes (3D rotation) was obtained by AC induced electric fields. • An open top biochip was successfully fabricated to enable further processing of the rotated cell in 3D spaces. • Bovine oocyte dielectric spectra were analysed in both in-plane and out-of-plane axes for the first time. • Bovine oocytes were determined to behave as solid spherical spheres, rather than single spherical shells.

No description available.

\Yith the current develop111ent in computer technology and Computational Fluid D)'n<'tlllics techniques, t.he si11utlation within axial flow compressors becomes 1110re and 1110re pract.ical and beneficial to the compressor designs. Due to the insufficient capabilit)' of today's COll1put.ers for three-dimensional unsteady flow 1110delling of 111Ult i~Llg(' axial flow compressors, sophisticated models of steady state flow and perfor111ance 1110delling of the C0111prcssors deserve to be thoroughly investigated. In l1utltistage C0111pressor sinlulations with steady state methods, frame of reference is fixed on blades and the c0111putational domains for rotors and stators haye relati\'e rotation. One of the difficulties in such simulations is how to pass information across the interfaces between blade rows without losing continuity. Two 111ajor stead)' state modelling approaches, a mixing plane approach based on Denton's circu111ferentially non-uniform mixing plane model and a deterministic stress approach based on Adamczyk's average passage model, are investigated and compared with each other through the flow predictions of the third stage of Cranfield Low Speed Research Compressor at peak efficiency operating condition. In the deterministic stress approach, overlapped solution domains are introduced to calculate deterministic stresses in order to 'close' the time-averaged governing equation system and the influence of the downstream blade row of the blade row under investigation has to be imposed through the simulation of bodyforce and blade blockage effect of the downstream blade row. An effective method of simulating bodyforce and blade blockage effect has been developed and proven to be simple in programming. ConYentionally, boundary conditions are specified in CFD calculations based on experimental data or other empirical calculations. By taking advantage of the special flow features in rear stages of multistage axial flow compressors where each rear stage behaves like a repeating stage of its neighbouring stages in terms of flow pattern at the inlet and the exit of these stages, a repeating stage model has been developed aiming at significantly simplifying the boundary conditions when simulating rear stages of a multistage axial flow compressor with only mass flow rate and stage exit average static pressure required as global input. A computer simulation system 1'/ STurbo3D has been developed to investigate a11d assess different steady state simulation models within multistage compressor environment. It has been proven that with the mixing plane model M STurbo3D is able to predict flows in multistage low speed axial flow compressors with acceptable accuracy. Application of the repeating stage model to the third stage of LS RC shows that the prediction with this model has equivalent accuracy to the prediction with the conventional boundary setting, and proves that the repeating stage model is an effective alternative to the expensive complete compressor simulation. The deterministic stress model provides more information of rotor-stator interaction and slightly better performance prediction than the mixing plane model, but the benefits of the model is not significant when applied to low speed axial flow compressors.

No description available.

No description available.