In last several years the amount of physical activities during our day time seriously decreased. That phenomenon was caused by reduction of physically demanding works in our jobs and sitting way of spending free time. The result of the previous fact is population fitness worsening and consequently worsening of the labour productivity and health condition. The fitness is tightly connected with the relationship between a man and a movement and sporting activities. It should become the important part of the healthy life style and influence the formation of proper manners of every single man. The fitness is one of the markers of the cardiovascular system condition. And it is considered to be the certain sum of preconditions for optimal reaction on the stress of whatever cause. The regular training, the physical activity and the daily reasonable energetic income are the safest and the least economically demanding ways how to prevent civilisation diseases. The first task of the theses was to assess the fitness of women students of The University of South Bohemia. The fitness was assessed on the base of Ruffier?s test. The second task was to compare the fitness of several groups of students which represented every single faculty of The University of South Bohemia. The both tasks were completed. Two hypotheses were settled and confirmed in purpose to reach the goal of the theses. H1: The numerological value of the Rufier?s test of women students of the Faculty of Education is lower than the result of women from the other faculties. H2: Women students whose result of Ruffier?s test is 0-5 are spending more time by performing physical activity than other students. In this theses, Ruffier?s test as a non-direct way of assessing the fitness has been chosen to confirm the both hypotheses. Ruffier?s test is a simple test to asses the fitness which uses changes of the hearth rate before and after exercising. The test is based on the general premise that the hard body exercising which last for a longer period of time is connected with the rising of heart rate. The heart rate was recorded by sport tester. Heart rate values were set into the pattern for counting the index of Ruffier?s test and consequently classified. Averages of the Ruffier?s index of each faculty were measured and compared. The questionnaire was ad to the Ruffier?s index for better assessing the fitness of students. The questionnaire was composed by thirteen questions. Some of questions were of open and some of close type. The questionnaires were anonymous. Hypothesis H1 was confirmed. The average value of the Ruffier?s index for women students of the pedagogical faculty was 10,7. And the average value of the Ruffier?s index for women students of other faculties was 13. It means the average fitness of women students of pedagogical faculty is better than the average fitness of women students of other faculties. Hypothesis H2 confirmed the correlation between the time spending by physical exercising and the better fitness. The more times weekly women students exercise for more than 30 minutes a day the lower value of the Ruffier?s index they have. The assumption of the average values of the Ruffier?s index of those women from 0 to 5 was overstated. Women student in this project had the average values from 10 to 15 which mean worse values of fitness. Despite the worse values of Ruffier?s index, the correlation between the spending time by exercising and better fitness was confirmed. To sum up, there is a recommendation to compensate the simple monotone physical activity by wide spectrum of physical activities. The sport should be introduced into the life style. The person with a good fitness has less problems with movement, can work for a longer time without break, is strong and capable to fulfil everyday tasks with reserves. The process of improving the fitness is a run for a long distance but it should be the goal of all people no matter of how old they are.