At present, cycling is at the forefront of smaller and larger cities. It is important to put emphasis on its development and the support of the infrastructure, in building new and existing cycling routes as well. An increasing number of cyclists on the roads causes a number of traffic accidents, especially with devices of transport. That is why to aim to increase the safety of cyclists themselves but also other road users. In my diploma thesis, I do evaluate the existing state of critical places on cycling routes and try to optimally solve the problems I have identified. It is mainly the connection of cycling infrastructure, for more comfortable ride, and increased safety of cyclists in Brno. The source I worked with was a bicycle transport manual of the Brno city.