The goals of the thesis are to present YouTube in the Czech Republic, to discuss its entire business model, analyze the main local competitors and mostly to suggest marketing strategy that will strengthen the position of YouTube as an online medium. The current trends in the internet society are introduced as well as the role of social media in order to understand how these factors are related to the position of YouTube in marketing. From general YouTube introduction the situation of this platform in the Czech market is analyzed. Based on this deep analysis, the key factors that should be taken into account in the Czech YouTube marketing strategy will be discussed.

Four methods were investigated to determine their suitability for use in a CF screening programme for the province of British Columbia. A fecal trypsin method which measured trypsin activity by incubating dry stool samples on filter paper cards with the substrate p-tosyl-arginine methyl ester (TAME) and a pH sensitive dye was shown to be non-specific and therefore unsatisfactory. An attempt to combine a fecal albumin screen with a more specific quantitative immunodiffusion technique for albumin and alpha-1 antitrypsin was unsuccessful. A meconium albumin assay using the Boehringer-Mannheim Corporation (BMC) test-strip and a more specific fecal trypsin assay which uses the substrate benzoyl-arginine-p-nitroanilide (BAPNA) were incorporated into two pilot projects at Children's Hospital in Vancouver. The BMC test-strip was simple to use, reliable and inexpensive. Of 8,891 infants tested, 3 positives were diagnosed as suffering from cystic fibrosis and 1 CF patient tested negative. False positives were obtained on 1.3% of infants. The incidence of CF as determined by this screen was 1 in 2000. The meconium albumin screen was satisfactory as a local pilot project but the disadvantages of testing the unstable meconium specimens make the screen unsuitable for a province-wide application. The BAPNA fecal trypsin method devised by Crossley was used to test 4085 dry stool specimens collected in the hospital and at home. Out of a total number of 190 positive results, none was diagnosed as having CF, giving a false positive rate of 5.0% for the hospital collected specimens and 3.4% for the specimens collected at home. The false positive rate in the hospital collected specimens was due mostly to the large proportion of young infants (under 3 days). The false positive rate of the home collected specimens appeared to be due mostly to the thinner spread of stool sample on the card. Because the quantity of stool sample per test was significantly lower in the home than the hospital collected specimens a new cut-off point for the home collected specimens was considered. Its application/ however did not lower the false positive rate sufficiently. As a result, the high incidence of false positives and the difficulties encountered as a result of this incidence also makes the fecal trypsin screen unsuitable for the province of B.C. Difficulties encountered during the follow-up of positive results obtained in the two pilot projects are discussed and recommendations are made regarding the efficient and adequate implementation of a follow-up system. / Medicine, Faculty of / Pathology and Laboratory Medicine, Department of / Graduate

A community health unit in a British Columbia suburb established a screening clinic for 3-year-olds modelled on similar programs already operating in nearby localities. The purpose of the study was to describe this new screening clinic: to report on the procedures employed, the personnel involved, the characteristics of the clientele, and the types, incidence, and disposition of problems detected amongst the children brought to the clinic. The study population consisted of the 47 children and their parents who were the clients of the clinic during its first three months. The research instruments were original questionnaires and forms devised to obtain sociological and health history information. The forms were completed during a home visit and by a telephone interview with each family. The data are arranged in frequency tables and percentages calculated where appropriate. A few variables are cross-tabulated to add descriptive depth to the study. The families in the study were from the middle and upper-middle class segment of society. They had frequently used other health resources in the community. The mothers' main concerns were about speech and language development and behavior problems of their children. Twenty-four children were referred by the clinic staff for 39 problems requiring retesting, further investigation, or intervention. Twelve of these referrals were for problems of vision, 10 for behavior, 6 for speech and language, 3 each for hearing, nutrition, and dental health, and 2 for physical developmental delay. The children cooperated enthusiastically in the test procedures and their parents found the clinic to be reassuring and a valuable learning experience. The parents were willing to comply with the referrals, but some delays in the follow-up procedures were noted, due to the newness of the clinic. The screening clinic for 3-year-olds appears to be filling a previously unmet need in the community. Community health workers involved in planning and promoting new services should find the detailed descriptions of the procedures and of the clientele of the clinic useful to them. The extensive bibliography provides a background of published material on the rationale and result of a variety of methods of screening. Comparison with similar clinics in other areas will be impossible until terminology used, test procedures employed, and methods of reporting results are standardized. Meanwhile, the clinic staff should continue to maintain statistical evidence of the results obtained and to evaluate the procedures used in its program. / Applied Science, Faculty of / Nursing, School of / Graduate

The business data could be considered as the raw material for decision-making process, for the development of corporate strategies and overall running of the business. Therefore, adequate attention should be paid to quality of the data. The main goal of the diploma thesis is elaboration of a specific framework for data quality assurance, which combines three theoretical concepts: time series analysis, data screening and data profiling -- business-specific data profiles are monitored by data screening during the data warehouse ETL (extract, transform and load) process and results are afterwards compared with the values predicted by time series analysis. Achievement of this goal is based on the analysis of "data quality" in literature, exact problem definition and selection of appropriate means for its solution. Moreover, the thesis is analysing alternative solutions available on the market and comparing their functionality with the functionality of own framework, as well.

The subject of this thesis is to identify and quantificate costs of Breast Cancer Therapy. The goal is the quantification of costs for specific therapeutic modalities, which are used by the therapy. Obtained values are used for presentation of the cheapest and the most expensive mode of treatment.

Nauclea latifolia Smith (Rubiaceae) est une plante aux vertus médicinales beaucoup plus connues en Afrique sub-saharienne dans la pharmacopée traditionnelle pour ses nombreuses activités pharmacologiques. N. latifolia Sm. est un arbre ou arbuste d'espèce soudano-sahélienne ; ses zones d'abondance sont principalement l'Afrique Occidentale et Centrale. Sa large utilisation en médecine traditionnelle , nous a incités à réaliser une enquête ethnobotanique et un screening phytochimique afin de mettre en cohérence les prescriptions et les activités potentielles des constituants chimiques présents. Les principales classes de métabolites secondaires, principalement les alcaloïdes, les flavonoïdes, les tanins, les stérols et terpéniques ont été recherchés dans les feuilles, écorces et racines de la plante. N. latifolia Sm. de par la présence des nombreuses familles chimiques, possède des activités biologiques intéressantes. Les extraits d'alcaloïdes totaux issus des 3 organes de la plante, ont prouvé une activité anticancéreuse. Ces extraits inhibent la prolifération des cellules cancéreuses mammaires MCF-7. Outre l'activité antitumorale, les extraits alcaloïdes de N. latifolia Sm. agissent contre la douleur. Ils inhibent en effet de la douleur après injection de l'acide acétique à 0,6 % chez la souris. Cependant, N. latifolia Sm. se présente comme une plante aux nombreux médicaments. Au regard de ces activités biologiques intéressantes, il convient de mener des investigations intenses pour enrichir la production des Médicaments Traditionnels Améliorés (MTA). / Nauclea latifolia Smith (Rubiaceae) is a plant with curative virtues much more known in Sub-Saharan Africa in the traditional pharmacopoeia for the numerous biological activities. N. latifolia Sm.is a shrub or tree Soudano-Sahelian species ; his zones of abundance are in most cases Western and Central Africa. His wide use in traditional medecine incited us to realize an ethnobotany investigation and a phytochemical screening to put in coherence the prescriptions and the potential activities of the chemical constituents. The main classes of metabolites secondary sectors, maily alkaloids, flavonoids, tannins, sterols and terpenoids, were looked for in the leaves, barks of stems and roots. N. latifolia Sm. all over the presence of its numerous chemical families have interesting pharmacological activities. The extracts of existent complete alkaloids of the 3 organs of the plant, proved an anticancerous activities. These extracts inhibit the proliferation of the mammary cancerous cells MCF-7. Besides, activity antitumorale extracts alkaloids of N. latifolia Sm. act against pain. They inhibit the effect of pain after injection acetic acid in 0,6 % to the mice. However, N. latifolia Sm. comes as a plant in numerous drugs. With regard to the interesting biological activities, it is necessary to lead intense investigation to enrich the production of Traditional Ameliorated Drugs.

No description available.

Nowadays, the organoids structures has become more popular as suitable model systems for clinical research, particularly for development of new medication and drug screening. The standard study approaches include invazive biochemical or molecular-biology analysis as well as non-invasive optical approaches. Among optical methods, various microscopy techniques can give a very detailed information about the structure of organoids. However, the microscopy is time consuming as well as it puts a great demand on instrumentation. Therefore, the microscopy is not suitable for high content analysis of multiple samples. This work is focused on the development of the device and experimental technique for high-throughput screenings of organoids structures for biomedical applications based on microtitrate plates. Literatre search for non-invasive optical methods, suitable for analysis of organoid structures. The necessary adjustements of existing system for algae phenotypization are discussed. An experiment was made to test functionality of designed system. Practical use for clinical use is tested by the experiment of spheroids reaction to selected cytostatics. The results and findings are discussed in the conclusion.

Drug discovery is a process that takes many years and hundreds of millions of dollars to reveal a confident conclusion about a specific treatment. Part of this sophisticated process is based on preliminary investigations to suggest a set of chemical compounds as candidate drugs for the treatment. Computational resources have been playing a significant role in this part through a step known as virtual screening. From a data mining perspective, availability of rich data resources is key in training prediction models. Yet, the difficulties imposed by big expansion in data and its dimensionality are inevitable. In this thesis, I address the main challenges that come when data mining techniques are used for virtual screening. In order to achieve an efficient virtual screening using data mining, I start by addressing the problem of feature selection and provide analysis of best ways to describe a chemical compound for an enhanced screening performance. High-throughput screening (HTS) assays data used for virtual screening are characterized by a great class imbalance. To handle this problem of class imbalance, I suggest using a novel algorithm called DRAMOTE to narrow down promising candidate chemicals aimed at interaction with specific molecular targets before they are experimentally evaluated. Existing works are mostly proposed for small-scale virtual screening based on making use of few thousands of interactions. Thus, I propose enabling large-scale (or big) virtual screening through learning millions of interaction while exploiting any relevant dependency for a better accuracy. A novel solution called DRABAL that incorporates structure learning of a Bayesian Network as a step to model dependency between the HTS assays, is showed to achieve significant improvements over existing state-of-the-art approaches.

This dissertation describes the development of a system for the automated, high-throughput screening of organic reactions. This system utilizes a liquid handling robot for reaction mixture preparation combined with desorption electrospray ionization mass spectrometry (DESI-MS) for reaction mixture analysis. With an analysis speed of ~1 second per reaction mixture, this system is capable of screening thousands of reactions per hour. Reaction mixtures are prepared in 384-well microtiter plates using a liquid handling robot. A sample of each reaction mixture (50 nL) is then transferred to a PTFE coated, glass slide using a pin tool. By offsetting the placement of the pin tool during each transfer, up to 6,144 unique reaction mixtures can be placed on each slide. The slide is then transferred to the DESI stage by a robotic arm, and the DESI-MS analysis begins, taking as little as 7 minutes for 384 reaction mixtures. We utilize a scheduling software to control each component of the system, which automates the entire process from reaction mixture preparation to DESI-MS analysis. In order to efficiently analyze and visualize the extremely large data sets generated by the system, we developed a custom software suite to automatically process each data set. We have used this system to screen several classes of industrially relevant reactions including Suzuki coupling, nucleophilic aromatic substitution, reductive amination, and Sonogashira coupling. We have validated both positive and negative results from the system using flow chemistry, and we have observed excellent agreement between the two methodologies. By being capable of screening thousands of reactions per hour, requiring only microliter quantities of reaction mixtures, and consuming less than a milliliter of solvent during the DESI-MS analysis, this system significantly reduces the time and costs associated with organic reaction screening.