Cyanobacteria can form nuisance proliferations and produce large concentrations of toxins that pose a health hazard. This thesis investigates cyanotoxin production by New Zealand benthic cyanobacteria. Cyanobacteria were sampled from lakes, reservoirs, streams, and rivers. Thirty-five strains were isolated into culture and screened for genes involved in the biosynthesis of common cyanotoxins. Positive results were confirmed and cyanotoxin concentrations quantified using analytical chemistry techniques. Genes involved in anatoxin a/homoanatoxin a biosynthesis were detected in nine out of ten Phormidium cf. uncinatum strains isolated from a single mat. Anatoxin a was confirmed in these strains by LC–MS/MS at concentrations from 0.3 to 6.4 mg kg⁻¹. One strain also produced homoanatoxin-a. Anatoxin-a variation between strains may explain the wide range in anatoxin a concentrations previously observed in New Zealand. The sxtA gene involved in saxitoxin biosynthesis was identified in Scytonema cf. crispum strains. Saxitoxin was confirmed in strains and environmental samples by Jellett PSP Rapid Test and HPLC–FD. Gonyautoxins, neosaxitoxin, and decarbamoyl derivatives were also detected. This study is the first identification of these compounds in Scytonema and in New Zealand cyanobacterial strains. These strains were isolated from recreational and pre-treatment drinking water reservoirs, highlighting the risk benthic cyanobacteria pose to human and animal health. Experiments were undertaken using cultures of Phormidium and Scytonema to determine how growth influences cyanotoxin production. The effects of iron and copper stress on P. autumnale were also investigated. High iron concentrations disrupted attachment mechanisms. Iron and copper had a significant effect on growth, without significantly affecting anatoxin a production. However, the maximum anatoxin a quota was consistently observed during early exponential growth. Scytonema cf. crispum produced higher saxitoxin quota throughout exponential growth than during the stationary phase. Both the Phormidium and Scytonema growth experiments indicate that high toxin quota can be expected early in benthic mat development, making early detection of these proliferations important.

Sedimentary characteristics of grainy non-skeletal tidal flats along windward platform margins have not been described in modern environments and may be misidentified or misclassified in the rock record. This study describes the sedimentology of such an environment to aid in accurate identification and characterization in the ancient. At Shroud Cay, a grain-dominated tidal flat is sheltered from the high energy of the shelf by a ring of cemented Pleistocene and partly indurated Holocene eolianite islands separated by several narrow tidal passes. Depositional texture, environment of deposition and geobody mapping, extensive sediment sampling, and vibracoring have shown that, though the cemented island provide a barrier from the high energy of the shelf, a high degree of tidal energy still occurs behind this barrier as indicated by the overwhelmingly grainy nature of all of the tidal flat sub-environments. Intertidal flats comprise the majority of the tidal flat surface. These flats are characterized by patchy Scytonema mats overlying bioturbated peloid-ooid grainstones to packstones with cemented lithoclasts. Three main tidal channels dissect the tidal flat and allow diurnal flow, one of those tidal channels does not exit the tidal flat but dead-ends behind a cemented Holocene beach dune ridge along the eastern side of Shroud Cay. Peloid-ooid-skeletal grainstone tidal bars and peloid-ooid packstones fill much of the channels. Most of the channels are bordered by low-relief grain-rich packstone levees often capped by red mangroves and algal mats. The interior-most supratidal parts of the flat, often in the lee of the windward Holocene ridge, are covered by a thick (5-~25cm) Scytonema microbial mat underlain by grain-rich ooid-peloid packstones. Ancient grain-dominated carbonate tidal flats and eolianite deposits like Shroud Cay?s are the reservoir rocks in some of today?s largest hydrocarbon fields. We develop a model for the evolution of the grain-dominated tidal flat, document and compare differences between the grain-rich tidal flat and surrounding environments of deposition, and develop a set of criteria for recognition. These criteria can be used to more accurately characterize reservoirs such as the Jurassic Smackover fields, to avoid mis-classification of similar settings, and more effectively produce those reservoirs.