Made available in DSpace on 2016-04-28T14:59:00Z (GMT). No. of bitstreams: 1 2009 - Mariluci Sudo Martelleto.pdf: 631667 bytes, checksum: 81045920c104dc187741ab864bd0fe1c (MD5) Previous issue date: 2009-04-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This study was conducted at the Laboratory of Pathology and Epidemiology, Department of Plant Science of UFRRJ and at PESAGRO-RIO / Serop?dica Experimental Station with the objective of selecting Trichoderma spp. antagonistic to Rhizoctonia in vitro; to quantify the effect of Trichoderma spp. in protecting seeds and seedlings of tomato against the attack of Rhizoctonia spp.; evaluate the effect of Trichoderma spp. in the emergence and quality of tomato seedlings developed in the presence of the pathogen and determine the macronutrient seedlings grown in a substrate inoculated with Trichoderma spp. and Rhizoctonia; study the effect of the combination of thermal and chemical treatments with microbiolization seeds with previously selected isolates of Trichoderma spp. and evaluate the survival of Trichoderma spp. in spermosphere and in the rhizosphere of seedlings. Were used isolates of Trichoderma spp, pre-selected from seeds of Santa Clara Miss Brazil , Garrafinha Vermelho and Perinha ?gua Branca , untreated with fungicide. Three of the isolates showed antagonistic activity in vitro and suppression of colony growth of Rhizoctonia spp. In tests of biological control under greenhouse conditions, effects of treatments were seen in the emergence, length and dry weight of shoot and root, root area of plants, teor and content of nitrogen, phosphorus and potassium, and easy recovery of Trichoderma spp. from seeds and roots of seedlings. For combination of thermal and chemical treatments of the seeds with Trichoderma spp. there went significant effects of treatments on emergence and seedling development. The seed treatment via dry heat at 70 ? C / 96 hours resulted in low germination both in Gerbox? and in the substrate, while treatments with water heated to 50 ? C for 30 minutes and with HCl 5% per 10 minutes were the least damage caused to the seeds. Seed treatment with hot water associated with isolated or TENA7 TENA16 as biological protectors resulted in good emergence and development of seedlings and saplings, as well as the association between treatment with 5% HCl for 10 minutes followed by application of Trichoderma isolate TENA7. / O presente trabalho foi realizado no Laborat?rio e Epidemiologia e Patologia de Sementes do Departamento de Fitotecnia da UFRRJ e na PESAGRO-RIO/ Esta??o Experimental de Serop?dica com o objetivo de selecionar isolados de Trichoderma spp. antagonistas a Rhizoctonia in vitro; quantificar o efeito da aplica??o de Trichoderma spp. na prote??o de sementes e pl?ntulas de tomate contra o ataque de Rhizoctonia spp; .avaliar o efeito da aplica??o de Trichoderma spp. na emerg?ncia e qualidade das mudas de tomate desenvolvidas na presen?a do pat?geno; determinar os teores de macronutrientes das mudas formadas em substrato inoculados com isolados de Trichoderma spp. e Rhizoctonia; estudar o efeito da associa??o de tratamentos t?rmicos e qu?mico com a microbioliza??o das sementes com isolados previamente selecionados de Trichoderma spp. e avaliar a sobreviv?ncia de isolados de Trichoderma spp. na espermosfera e rizosfera das mudas. Utilizaram-se isolados de Trichoderma spp. previamente selecionados e sementes das cultivares Santa Clara Miss Brasil e Garrafinha Vermelho sem tratamento pr?vio com fungicida. Tr?s dos isolados testados apresentaram antagonismo in vitro e supress?o do crescimento das col?nias de Rhizoctonia spp. Nos ensaios de controle biol?gico sob condi??es de casa-de-vegeta??o, foram observados efeitos dos tratamemtos na emerg?ncia, comprimento e massa seca de parte a?rea e ra?zes, ?rea radicular das mudas, nos teores e conte?dos de N, P e K e f?cil recupera??o dos isolados de Trichoderma spp. a partir das sementes e ra?zes das mudas. Na associa??o de tratamentos t?rmico e qu?mico das sementes com isolados de Trichoderma spp. houve efeito significativo dos tratamentos sobre a emerg?ncia e desenvolvimento das mudas. O tratamento das sementes via calor seco a 70?C/ 96 hs resultou em baixa germina??o de sementes tanto, em gerbox quanto em substrato, enquanto os tratamentos com ?gua aquecida a 50? C por 30 min e com HCl a 5% por 10 min foram os que menos danos causaram ?s sementes. O tratamento de sementes com ?gua quente associado aos isolados TENA7 ou TENA16 como protetores biol?gicos resultaram em boa emerg?ncia e desenvolvimento das pl?ntulas e mudas, assim como a associa??o entre tratamento com HCl a 5% por 10 min seguido da aplica??o do isolado TENA7 de Trichoderma.