<p>Germination testing and seedling vigor for Kentucky bluegrass germination. Analyzing growth from Kentucky bluegrass cultivars. </p>

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Rosebay rhododendron (Rhododendron maximum L.) is an evergreen ericaceous shrub that plays a dynamic role in the southern Appalachian forests. Commonly located on mesic sites, this understory shrub forms dense thickets that greatly reduce the amount of light available to herbaceous and woody plants found on the forest floor. Past research has shown that silvicultural methods can be used to eradicate R. maximum, however it is unclear which of these methods is most efficient and what effects other than stem mortality may occur. In this study, treatments involving prescribed fire, mechanical cutting, and herbicide applications were applied to R. maximum dominated forests in southwestern Virginia to determine what effect seven different silvicultural treatments had on 1) controlling of R. maximum as a forest weed 2) fuel loading inside of a R. maximum thicket, and 3) canopy tree seedling regeneration. Mechanical cutting treatments were successful in reducing R. maximum basal area per acre; however stump sprouting and increased fuel loading occurred. Herbicide applications were successful in controlling only the smallest diameter class of R. maximum stems. Prescribed fire reduced litter layers and caused delayed mortality on R. maximum stems three years following treatment. Hemispherical photographs taken within each plot showed that silvicultural treatments that successfully increased the amount of light entering each plot were influential in seedling establishment three years following treatments. Results from this study can be used to further perfect silvicultural applications that alleviate R. maximum cover on the forest landscape. / Master of Science

Survival and growth of planted trees are common indices used to evaluate success of wetland restoration efforts used to compensate for wetland losses. Restoration efforts on marginal agricultural lands typically result in less than satisfactory survival and growth of desired tree species. This study evaluated seed source ecotype, greenhouse preconditioning and combinations of five mechanical site preparation techniques (mound, bed, rip, disk, pit), four levels of planting stock (gallon, tubeling, bare root, and direct seed), and three planting aids (mat, tube, none) on the survival and growth of American sycamore (Platanus occidentalis L.) and willow oak (Quercus phellos L.) planted on an old field riparian area in the Virginia Piedmont. American sycamore seedlings subjected to greenhouse flood preconditioning had 25% greater height and willow oak seedlings grown under normal greenhouse conditions had 18% greater diameter, but these greenhouse adaptations did not confer greater survivability or growth after field planting. American sycamore seeds sourced from dry ecotypes were 14% taller than wet ecotype seeds, and willow oak acorns sourced from wet ecotypes were 11% taller than dry ecotype acorns, indicating that parental ecotype may influence survivability and growth. The combination of mounding site preparation and gallon planting stock increased mean survival to 100% and aboveground dry biomass (5.44 Mg/ha/yr) in American sycamore. Willow oak had 45% greater woody stem volume with mounding site preparation 80% greater woody stem volume with gallon and bare root planting stock. Tubeling planting stock provided significant benefit relative to the low planting stock cost for American sycamore, while bare root seedlings were shown to be an effective planting stock for willow oak. The use of appropriate ecotype seed sources, use of mounding mechanical site preparation techniques and planting of species appropriate planting stock increased survival and growth of common early and mid-successional Piedmont tree species on marginal agricultural lands. Treatments that appear to be economically viable for restoration and mitigation efforts could potentially offer other economic incentives such as short rotation woody crops and timber value, which might induce additional private landowners to attempt restoration efforts in marginal old field riparian areas. / Doctor of Philosophy / In order to offset the loss of wetlands due to development, strategies are needed to create wetlands in areas along streams that are unfarmed. Survival and growth of planted trees are commonly used to evaluate the success of these new wetland areas. The goal of this research is to provide alternative methods to increase survival and growth of two common trees planted in the Piedmont of Virginia. The results show that creating mounds of soil before planting trees and planting larger trees will increase tree survival and growth in these wetland areas. Landowners and land managers can use these methods to increase the value of unfarmed land along streams while also increasing water quality and providing habitat animals that use the streams and wetlands.

The successful regeneration of mature oak (<i>Quercus</i> spp.) forests is thought to be dependent on the presence of oak advance regeneration. However, the advance regeneration must be of sufficient size and density for oak to be competitive and become a dominant species after harvest. The presence of a dense midstory canopy of shade tolerant species has been implicated with the poor development of oak advance regeneration. Understory Vegetation Control was conducted in 1994 in average quality (SI₅₀ 17.7 - 21.9 m for upland oak) southern Appalachian forest stands to determine the effects on oak (<i>Quercus</i> spp.) and maple (<i>Acer</i> spp.) advance seedling abundance, growth, and development. Three study sites were located in southwest Virginia, and the following two treatments were implemented at each site: Understory Vegetation Control (UVC) and Control. Permanent sampling plots and individual seedlings were located to quantify the density, recruitment, and growth of advance regeneration. Competing vegetation was significantly reduced after one year by the UVC treatment, where the mean relative change in the sum of the heights of competing stems between 1 and 5 m in height was -15.9 percent for the UVC plots vs. 22.8 percent for the Control plots. Neither oak seedling recruitment nor height growth was enhanced by the UVC treatment after one year. Insufficient time has elapsed to allow for a growth response, or to determine if seedling recruitment will be enhanced by UVC. Several years may be required to determine if the UVC treatment can enhance the growth and competitive status of oak regeneration. / Master of Science

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Bien que les interactions biotiques soient connues pour être déterminantes dans l’établissement des espèces, il est encore difficile de savoir quels facteurs sont impliqués dans l’équilibre entre interaction positive et interaction négative. Il est de fait difficile de savoir sous quelles conditions les interactions biotiques peuvent favoriser ou empêcher la régénération des espèces. Cette thèse vise à étudier le rôle des interactions biotiques d'arbustes avec des semis de chêne sur la régénération de trois espèces de chênes sur les forêts des dunes côtières d'Aquitaine en testant comment l’effet net de ces interactions varie le long d’un gradient d’aridité, sur deux étages de canopée et en fonction des stratégies fonctionnelles de trois espèces de Chêne dans un contexte de changement global. Ce travail a été effectué en utilisant deux approches, une approche descriptive en utilisant un patron de points répartis dans l’espace et une approche expérimentale en transplantant les semis cibles. Les résultats montrent que la variation spatiale, en terme d’interactions biotiques, est fortement corrélée avec la sévérité environnementale, avec des interactions entre jeunes pousses de chêne très sensibles aux sécheresses estivales et aux trouées dans les canopées. Les interactions testées étaient de nature facilitatrice dans les plots découverts dans les dunes sèches du nord de Soulac et tournaient à la compétition sous le couvert forestier dans les dunes plus humides du sud, à Seignosse. La nature des interactions était constant entre les stratégies fonctionnelles des espèces cibles de chêne. Les résultats de cette thèse montrent de manière générale une confirmation de la formulation originale du SGH qui prédit une augmentation de la facilitation en lien avec une augmentation de la sévérité environnementale et souligne le fait que la réduction du stress hydrique atmosphérique par des arbustes est nécessaire à la régénération des semis de chêne. Dans cette perspective, le sylviculteur doit conserver les arbustes du sous-étage, en particulier dans les trouées, afin de permettre une meilleure régénération des plants de chêne. Cette thèse met en évidence la nécessaire considération des interactions biotiques dans la régénération du chêne dans les actuelles sévères conditions climatiques et le rôle prépondérant de ces interactions dans la réponse aux changements climatiques futurs probables dans cette région Aquitaine. / Although biotic interactions are known to be important determinants of species establishment, it is uncertain what factors determine the net balance between positive and negative interactions thus, under what conditions biotic interactions could enhance or impede species regeneration. Bien que les interactions biotiques soient connues pour être This thesis aims to study the role of biotic interactions of shrubs with oak seedlings for regeneration of three oak species on the Aquitaine coastal dune forests, by testing how the net effect of these interactions vary along aridity gradient, between two overstory canopies and in respect to the functional strategies of three oak species in the context of climate change. This was done by using two approaches, descriptive approach using spatial point pattern data and experimental approach by transplanting the target seedlings. The results show that the spatial variation in the nature of biotic interactions is strongly relate to environmental severity conditions, where the shrub-oak seedling interactions were very sensitive to increasing summer drought and canopy opening, the interactions strength was facilitative under gap plots in the dry northern dunes in Soulac and switch on competitive under forest plots in the wet southern dunes in Seignosse. The nature of the interactions was constant across the functional strategies of the targets species of oak. For the most part, results of this thesis show general support to the original formulation of SGH which predicts increasing facilitation with increasing severity and underscore the fact that atmospheric water stress reduction by shrubs is required for oak seedling regeneration. In this perspective, silviculturist should conserve understory shrubs, in particular in gaps, in order to allow a better regeneration niche of oak seedlings. This thesis highlights the importance of considering biotic interactions in oak regeneration under current harshness climatic conditions and with expectation to have an ambitious role in alleviation future climatic change consequence in this region.