This thesis “Increasing the nutritional value of intergeneric hybrids of Festulolium for fodder by non-root application of selenium” describes a one-year study on the reactions of four varieties of Festulolium to biofortification with selenium in a small plot. All variants of the fodder were fertilized at a standard nitrogen (N) dose of 60 kg.ha-1, and selenium was applied foliarly as Na2SeO3 in at an offshoots phase at 16 g.ha-1 and 32 g.ha-1. Non-root applications of selenium, especially at a lower doses of 16 g ha-1 is statistically proven to be an appropriate measure to increase yield and increase selenium content and nutritional values (dry matter, nitrogenous substances, fibre, WSC and NEL) in fodder of both loloid and festucoid types of Festulolium. The most effective influence on clayey hybrids (Festulolium braunii) was exerted through spraying selenium onto the leaves at a dose of 16 g.ha-1, while a dose of 32 g.ha-1 exerted a greater effect on fescue (Festulolium krasanii). Biofortification of the observed fodder plant species by selenium was proven to be a valid means of enriching fodder with a consequent positive effect in the food chain.