This dissertation was 2 parts of investigations of various acute responses to high-volume combined accentuated eccentric loading and rest redistribution (AEL + RR). Resistance-trained men (n = 12, 25.6 ± 4.4 years, 1.77 ± 0.06 m, 81.7 ± 11.4 kg) completed a back squat (BS) 1 repetition maximum (1RM) and weight releaser familiarization session. Three BS exercise conditions (sets × repetitions × eccentric/concentric loading) comprised AEL + RR 5: 3 × (5 × 2) × 110/60%, AEL + RR 2: 3 × (2 × 5) × 110/60%, and traditional sets (TS): 3 × (1 × 10) × 60/60% 1RM. The AEL + RR 5 resulted in significantly (p < 0.05) greater concentric peak velocity (PV) (1.18 ± 0.17 m∙s–1) and power (PP) (2,304 ± 499 W) compared to AEL + RR 2 (1.11 ± 0.19 m∙s–1 and 2,148 ± 512 W) and TS (1.10 ± 0.14 m∙s–1 and 2,079 ± 388 W). Furthermore, AEL + RR 5 resulted in significantly greater PV and PP across all 10 repetitions compared to TS. Although AEL + RR 5 resulted in significantly greater concentric mean force (MF) (1,706 ± 224 N) compared with AEL + RR 2 (1,697 ± 209 N) and TS (1,685 ± 211 N), no condition by set or repetition interactions were found. The AEL + RR 5 yielded greater total volume load (sets × repetitions × eccentric + concentric loading) (6630.1 ± 1210.4 kg) compared with AEL + RR 2 (5944.3 ± 1085.2 kg) and TS (5487.0 ± 1001.7 kg). The AEL + RR 5 led to significantly (p < 0.05) greater rating of perceived exertion (RPE) after set 2 and 3 and lower blood lactate (BL) after set 3, 5-, 15-, and 25-minute post-exercise than AEL + RR 2 and TS. There was a main effect of condition for BL between AEL + RR 5 (5.11 ± 2.90 mmol∙L–1), AEL + RR 2 (6.23 ± 3.22 mmol∙L–1), and TS (6.15 ± 3.17 mmol∙L–1). In summary, AEL + RR 5 results in unique acute mechanical, physiological, and perceptual responses in high-volume BS exercise.