DNA methylation patterns are sex and tissue specific in many species, yet many studies useblood samples, due to its accessibility, to establish links between the DNA methylation anddifferent phenotypes. This raises the question of whether DNA methylation in blood samplesreflect the DNA methylation pattern in other tissues that are more relevant to the phenotypebeing studied. In this research, samples were collected from the brain, blood, liver and gonadof 16 house sparrow (Passer domesticus), half of them were female, while the others weremale. Reduced representation bisulfite sequencing (RRBS) was performed to get themethylation profile in each sample. The result showed a tissue specific methylation profile inthe four investigated tissues, a strong and positive correlation between 0.74 – 0.85 was foundbetween tissues, in which a weaker correlation was found between blood and other tissue. Indifferential methylation analysis, most of the differently methylated sites between sexes werefound in gonads, while the fewest was found in blood, and Z chromosome wasoverrepresented place in all four tissues where the majority of the differently methylated sitesbetween sexes were found. Comparison with the house sparrow genome annotation foundabout half of the differentially methylated sites between sexes were within genes and about 20 % of them were in the exon or coding region of a gene. The result suggested that bloodcould be useful in reflecting the general DNA methylation level in other tissues, but it was nota reliable bioindicator for further detailed study in DNA methylation pattern or in geneontology enrichment pathway analysis.