Sexual assault is a growing concern across college campuses in the United States. According to the Sexual Victimization of College Women study, the victimization rate is 27.7 rapes per 1,000 ­­­­women students (Fisher, Cullen, & Turner, 2000). In response to the high prevalence of sexual assault, college campuses are now mandated to implement various forms of sexual assault prevention programming. Sexual assault prevention programming is intended to promote awareness of sexual assault and reduce the prevalence of sexual assault on college campuses. Numerous studies have examined the short term effectiveness of sexual assault prevention programs (e.g., Anderson & Whiston, 2005). However, few studies have explored the effectiveness of repeated, annual prevention program participation on rape supportive beliefs. In addition, studies that have explored the efficacy of prevention programs have tended to rely exclusively on self-report measures and some have only focused on outcomes among men or women groups only. The purpose of the present study was to examine the impact of level of participation and gender in sexual assault prevention training on rape myth acceptance (RMA) and response latency to a hypothetical date rape scenario among a sample of Western college students. Results revealed no significant relationships between higher levels of sexual assault prevention programming participation and RMA scores and latency times. Additionally, there were no gender differences on IRMA scores or response latency. Findings have important implications for future sexual assault prevention programming efforts on college campuses and community settings.