Amostras de Plesiomonas shigelloides, isoladas de água de rio, produziram uma citotoxina denominada LCF (Fator Citotóxico Letal), ativa em células Vero, CHO, CaCo-2 e HT-29, causando vacuolizações intracelulares e retração dos núcleos, levando á morte por apoptose. Esta citotoxina foi purificada e seu peso molecular estimado em 48 kDA. É uma citotoxina termolábil e foi parcialmente neutralizada por antisoro anti-LT-1 (enterotoxina termolábil tipo 1 de Escherichia coli) e pelo antisoro anti-Aerolisina de Aeromonas. O antisoro anti-CT (enterotoxina colérica) não neutralizou sua atividade. Em teste \"Western blot\" para anti-LT-1, não ocorreu reação sorológica. O LCF induziu acúmulo de fluido em intestino de camundongos recém-nascidos e alças intestinais de coelhos. O LCF foi letal para camundongos e ratos, levando-os a morte cardíaca súbita quando injetado por via intravenosa. Considerando os resultados obtidos, neste estudo, as amostras de P. shigelloides isolados de água de rio, mostraram grande potencial como patógeno para o homem e animal. / Plesiomonas shigelloides is a ubiquous microorganism recognized as putative human and animal enteropathogen. Production of toxins has been related to their role in pathogenicity. At previous studies we detected a cytotoxin, named LCF (Lethal Citotoxic Factor) active on a variety of cells, causing intensive intracellular vacuolation and nuclear condensation, leading to death. The toxin purification revealed a heat-labile protein of about 48 kDa by electrophoresis analysis in poliacrylamide gel (SDS-PAGE). The cytotoxic activity was partially neutralized by anti-LT-1 (type 1 termolabile enterotoxin of Escherichia coli) and by anti-aerolisin (cytotoxic enterotoxin of Aeromonas). Western blot analysis to anti-LT-1 showed no serological reaction. Fluid accumulation occurred when toxin was applied to the intestine of newborn mice and to rabbit intestinal loop assays. LCF was lethal to mice and rats, leading them to cardiac death when injected intravenously. Considering the results, these isolates of P. shigelloides have shown great potential as pathogens.

Amostras de Plesiomonas shigelloides, isoladas de água de rio, produziram uma citotoxina denominada LCF (Fator Citotóxico Letal), ativa em células Vero, CHO, CaCo-2 e HT-29, causando vacuolizações intracelulares e retração dos núcleos, levando á morte por apoptose. Esta citotoxina foi purificada e seu peso molecular estimado em 48 kDA. É uma citotoxina termolábil e foi parcialmente neutralizada por antisoro anti-LT-1 (enterotoxina termolábil tipo 1 de Escherichia coli) e pelo antisoro anti-Aerolisina de Aeromonas. O antisoro anti-CT (enterotoxina colérica) não neutralizou sua atividade. Em teste \"Western blot\" para anti-LT-1, não ocorreu reação sorológica. O LCF induziu acúmulo de fluido em intestino de camundongos recém-nascidos e alças intestinais de coelhos. O LCF foi letal para camundongos e ratos, levando-os a morte cardíaca súbita quando injetado por via intravenosa. Considerando os resultados obtidos, neste estudo, as amostras de P. shigelloides isolados de água de rio, mostraram grande potencial como patógeno para o homem e animal. / Plesiomonas shigelloides is a ubiquous microorganism recognized as putative human and animal enteropathogen. Production of toxins has been related to their role in pathogenicity. At previous studies we detected a cytotoxin, named LCF (Lethal Citotoxic Factor) active on a variety of cells, causing intensive intracellular vacuolation and nuclear condensation, leading to death. The toxin purification revealed a heat-labile protein of about 48 kDa by electrophoresis analysis in poliacrylamide gel (SDS-PAGE). The cytotoxic activity was partially neutralized by anti-LT-1 (type 1 termolabile enterotoxin of Escherichia coli) and by anti-aerolisin (cytotoxic enterotoxin of Aeromonas). Western blot analysis to anti-LT-1 showed no serological reaction. Fluid accumulation occurred when toxin was applied to the intestine of newborn mice and to rabbit intestinal loop assays. LCF was lethal to mice and rats, leading them to cardiac death when injected intravenously. Considering the results, these isolates of P. shigelloides have shown great potential as pathogens.

<p>U ovom radu vr&scaron;ena je izolacija sojeva<em>&nbsp; P. shigelloides</em>&nbsp; iz uzoraka povr&scaron;inskih voda<br />Panonske nizije i formirana je kolekcija kultura, nakon čega je obavljena karakterizacija<br />faktora virulencije i antibiotske rezistencije&nbsp; izolovanih sojeva. Pored toga vr&scaron;ena je<br />izolacija bakteriofaga specifičnih za vrstu <em>P. shigelloides</em> iz uzoraka povr&scaron;inskih i otpadnih voda kao i njihova karakterizacija. Karakterizacija je podrazumevala sledeće testove: ispitivanje efikasnosti lize, litičkog spektra, uticaja različitih ekolo&scaron;kih faktora,<br />određivanje proteinskog profila i karakterizaciju genoma. Pored toga, vr&scaron;ena je detekcija<br />izolovanih bakteriofaga kori&scaron;ćenjem protočnog citometra. Rezultati ovog rada su potvrdili<br />prisustvo bakterije <em>P. shigelloides</em>&nbsp; i njenih specifičnih bakteriofaga u povr&scaron;inskim&nbsp; vodama Panonske nizije. Budući da su bakteriofagi specifični za vrstu&nbsp;<em> P. shigelloides&nbsp;</em> po prvi put izolovani, rezultati ovog rada mogu predstavljajati prvi&nbsp; korak u ispitivanjima vezanim za regulaciju brojnosti ove bakterije putem primene bakteriofaga.</p> / <p>We analyzed surface waters collected in Panonian plane, for the presence of bacterium&nbsp; <em>P. shigelloides</em>. The bacterial strains were preserved and characterized for virulence factors and antibiotic resistance. In addition, isolation of&nbsp; <em>P. shigelloides</em>&nbsp; specific bacteriophages was performed. Characterization of isolated phages included the following tests: examination of lytic efficacy, lytic spectrum, the influence of various ecological factors, the determination of the protein profile and the characterization of the genome. In addition, isolated bacteriophages were detected using a flow cytometer. The results of this paper demonstrate that the surface waters of Panonian plane contain bacterium&nbsp; <em>P. shigelloides&nbsp;</em> and its specific bacteriophages. Since&nbsp; <em>P. shigelloides</em>&nbsp; &ndash;&nbsp; specific bacteriophages are for the&nbsp;first time isolated, the results of this paper can represent the first step in the studies related to the regulation of the number of this bacterium by the application of bacteriophages.</p>

Diss. (sammanfattning). Uppsala : Sveriges lantbruksuniv., 2003. / Härtill 5 uppsatser.

Diss. (sammanfattning). Uppsala : Sveriges lantbruksuniv. / Härtill 4 uppsatser.

Crohn’s disease is an inflammatory bowel disease that may involve any part of the gastrointestinal tract with a variety of extraintestinal features. A flare of Crohn’s disease may present as partial small bowel obstruction or peritonitis. Dehydration, infectious agents, and cigarette smoking are some of the factors linked to a relapse of Crohn’s disease. Plesimonas Shigelloides, a bacterium that belongs to the enterobacteriaceae group may rarely lead to a flare of Crohn’s disease. We describe the case of a 31-year-old male with Crohn’s disease who developed a flare triggered by Plesimonas Shigelloides infection presenting as partial small bowel obstruction with ileal narrowing, and regional lymphadenopathy that responded to immunosuppressants.