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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Investigations of CHO 1-15 and Silk Gland Cell Line Development

Robinson, Susan Kathleen 01 May 2014 (has links)
The BioProcessing Demonstration and Training Laboratory was established with a collaboration between Utah State University and Thermo Fisher- Scientific, Inc. This lab was developed into a fully functioning tissue culture facility. Demonstration of tissue culture procedures were necessary for the lab to be industry-fully qualified. The CHO 1-15 cell line protocols were optimized by establishing conditions for reproducibility in shaker flasks and bioreactors (2 to 250 L capacity). CHO 1-15 is the cell line of choice for protein production in the bio-manufacturing industry. Oxidative stress is a problem in the industry because it can cause a decrease in protein production. Mesobiliverdin IXα and biliverdin IXα possess antioxidant capabilities. The effects of the antioxidant nature of these compounds were tested on the CHO 1-15 cell line. Cell cultures from silkworm and spider silk producing cells were also pursued. Methods to produce a primary cell line from spider silk gland cells were developed. Cell lines from spider and silkworm silk producing glands appeared to have the capacity to secrete full-length native proteins ranging in size from 200 to 500 kDa, and possibly larger.

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