Ethyl glucuronide (EtG) is a minor ethanol metabolite that confirms the absorption and metabolism of ethanol after oral or dermal exposure. Human data suggest that maximum blood EtG (BEtG) concentrations are reached between 3.5 and 5.5. h after ethanol administration. This study was undertaken to determine if the Sprague-Dawley (SD) rat biotransforms ethanol to EtG after a single high oral dose of ethanol. SD rats (male, n=6) were gavaged with a single ethanol dose (4g/kg), and urine was collected for 3. h in metabolic cages, followed by euthanization and collection of heart blood. Blood and urine were analyzed for ethanol and EtG by gas chromatography and enzyme immunoassay. Blood and urine ethanol concentrations were 195 ± 23 and 218 ± 19. mg/dL, whereas BEtG and urine EtG (UEtG) concentrations were 1,363 ± 98. ng equivalents/mL and 210 ± 0.29. mg equivalents/dL (X̄±standarderrorofthemean[S.E.M.]).Sixty-six male SD rats were gavaged ethanol (4. g/kg) and placed in metabolic cages to determine the extent and duration of ethanol to EtG biotransformation and urinary excretion. Blood and urine were collected up to 24. h after administration for ethanol and EtG analysis. Maximum blood ethanol, urine ethanol, and UEtG were reached within 4. h, whereas maximum BEtG was reached 6. h after administration. Maximum concentrations were blood ethanol, 213 ± 20. mg/dL; urine ethanol, 308 ± 34. mg/dL; BEtG, 2,683 ± 145. ng equivalents/mL; UEtG, 1.2 ± 0.06. mg equivalents/mL (X̄±S.E.M.). Areas under the concentration-time curve were blood ethanol, 1,578. h*mg/dL; urine ethanol, 3,096. h*mg/dL; BEtG, 18,284. h*ng equivalents/mL; and UEtG, 850. h*mg equivalents/dL. Blood ethanol and BEtG levels were reduced to below limits of detection (LODs) within 12 and 18. h after ethanol administration. Urine ethanols were below LOD at 18. h, but UEtG was still detectable at 24. h after administration. Our data prove that the SD rat biotransforms ethanol to EtG and excretes both in the urine and suggest that it is similar to that of the human.