Often on cattle operations and dairy farms, where multiple bulls are exposed to cows either by live cover or artificial insemination, error can be present in parentage record keeping for breed registries or production use. Research has evolved to the integration of using single nucleotide polymorphisms (SNPs) to answer questions where cases of unknown parentage may exist. With the evolution of the research, differentiated panels have been created specifically for parentage analysis. Our objective was to complete an analytical comparison between two specific panels, a proven 88 parentage SNP panel and a recently developed 25 SNP panel. A smaller panel would be beneficial in a parentage test as the smaller panel would reduce time and costs associated with the parentage analysis. In this study, nearly 4,000 cattle samples were collected and prepared from offspring, sires, and dams. Parentage calling software was used to identify the parentage assignments of the samples. Through these procedures and comparisons, it was determined that the smaller 25 SNP panel did not have the magnitude or strength necessary to be able to correctly identify cattle in the same manner as the 88 SNP panel.

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