The aim of this dissertation was to assess the influence of characteristics important for ensuring of sustainable and development of dairy cattle herds breeding. The data investigated describe approximately 5138 dairy cows in period of four years in selected farming´s of dairy cattle in district of Jičín. There were these indicators of reproduction processed: conception rate after first insemination, conception rate after all inseminations, interval of insemination, index of insemination, service period and meantime; the data were collected from seven stables of Bohemian Spotted cattle and from seven stables of Holstein cattle. The longevity was investigated from the number of dairy cows at respective lactation of both investigated breeds and from the average lactation in respective years. The results of investigation prove that the average percentage representation of dairy cow at respective lactation between 2012-2015 ranged 33,4 % at first lactation, and 24,68 % at second lactation, and 18,13 % at third lactation, and 11,48 % at fourth lactation, and 6,53 % at fifth lactation, and 3,32 % at sixth lactation, and 2,36 % at seventh and more lactations. The evident data from comparison prove, that till third lactation there is higher percentage representation of Holstein cattle and from third lactation there are more cows of Bohemian Spotted cattle, and also the average reached lactation confirms this the value of 2,75 lactation for Bohemian Spotted cattle and the value of 2,3 lactation for Holstein cattle. The influence of breed on the average length of lactation was quite weak. The results of rating indicators of reproduction also have not proven any statistically important difference between both types of cattle as concerns values reached by each cattle, but the original Bohemian Spotted cattle reached better results in all indicators, and thus it is probably more suitable for the area of district Jičín.