The conversion of natural habitat to urban areas has lasting impacts on wildlife and biodiversity. Known effects to urban wildlife include direct mortality while crossing roads, reduced species diversity, and habitat fragmentation and degradation. Among wildlife occupying urban areas, turtle populations can be particularly impacted in anthropogenic landscapes. Snapping Turtle (<i>Chelydra serpentina</i>) is one of the most common species found within urban wetlands, but populations are beginning to show declines in northern portions of their geographic range. The preservation and management of this species is aided by knowledge related to its spatial ecology. I investigated <i>C. serpentina</i> home range, movement, habitat use, and habitat selection in a midwestern USA urban wetland complex during two active seasons (May-August 2019 and 2020) using radiotelemetry. Home range sizes and movement did not differ between sex or sample year except the mean movement of males decreased from 2019 to 2020. No differences in mean monthly movement were found between sexes but mean monthly movement did differ between month and year. Habitat use was skewed during the active season and did not differ between sex or year, but there were positive habitat associations between forested wetlands and modal centers of activity (MCA). Habitat selection was tested at two spatial scales by comparing random points to home ranges and turtle locations using Euclidean Distance Analysis. Turtles appeared to select home ranges from available habitat site-wide but did not select habitat within home ranges. Home range selection included semi-permanent open water, trail, road/barrier, permanent open water, scrub-shrub, ditches, shoreline, and vegetated ponds, while upland forest, field and agriculture habitat were avoided. Home ranges appear to be constrained by available habitat and movement differences between years may be due to anthropogenic change in water levels. The use of space seems to be more affected by wetland size and connectivity than proximity to barriers, which suggests that management practices that protect turtles from accessing roads and railways will benefit populations. Additionally, habitat selection and association indicate that ditches are utilized as corridors between wetland areas. When feasible, increasing the connectivity of large wetlands containing many habitat types should have positive impacts on the persistence of populations in human dominated landscapes.

In Ontario, the common snapping turtle, Chelydra serpentina, is listed as Special Concern and is at risk of decline. These long-lived organisms are particularly susceptible to anthropogenic threats owing to delayed sexual maturity, low recruitment and reliance on low adult mortality. Threats facing this species include road mortality and habitat loss and/or destruction. These threats are anecdotally evident within Cootes Paradise Marsh, a highly urbanized river-mouth coastal marsh located in a heavily urbanized region at the western end of Lake Ontario. Straddling Dundas and Hamilton, the wetland is bisected by Cootes Drive, a four-lane highway (maximum speed 80 km/h) that has resulted in fatal collisions with wildlife. We re-analyzed data from previous capture-mark-recapture studies and determined that the snapping turtle population has declined by almost 90% from 1985 to 2002. Using road mortality survey data collected by citizen science group Dundas Turtle Watch, we determined that some of this decline can be attributed to road mortality. Through radio tracking, we also found that the population remains at risk to road mortalities because their home ranges overlap surrounding roads. Besides the direct negative effect of mortality, roads impart broader and additional unintended consequences by hindering turtles’ access to critical nesting and overwintering habitat. We acquired digital orthophotos to assess changes in availability of nesting habitat, which is crucial for recruitment and population maintenance. Potential nesting habitat decreased by almost 50% between 1934 and 2010. Through nest surveys conducted in 2017, we determined that there is currently disproportionate use of artificial gravel mounds for nesting. For overwintering purposes, snapping turtles appear to be using a wide range of habitat types within the upland terrestrial matrix of the wetland ecosystem. We also confirmed that sites used for overwintering did not cool below inhibiting temperatures between early December and end of March. This study illustrates the toll that road mortality can have on urbanized herpetofauna populations and highlights the importance of ensuring that recovery plans focus on restoring both the quantity and quality of nesting and overwintering habitats. / Thesis / Master of Science (MSc) / Common snapping turtles (Chelydra serpentina) exhibit “slow” life history traits which make them particularly vulnerable to human activities, such as urbanization. In this thesis, I used demographic and road mortality information to provide evidence that the population of snapping turtles living in the degraded Cootes Paradise Marsh ecosystem has substantially declined over the past three decades. I also assessed current availability of nesting and overwintering habitat that are both essential for maintaining a viable population of snapping turtles. My results highlight the devastating effects road mortality can impart on long-lived freshwater reptiles, and cautions against further modification or destruction of their critical habitat.