Soil health-based agricultural management practices are widely promoted to improve soil structure, infiltration and reduce erosion. This study was conducted at two locations in North-Central Mississippi to evaluate the influence of different cover crop species and poultry litter on soil health that can impact crop production, climate change, and resilience. The results indicated that the cover crops showed a little effect on some soil health indicators compared to control treatment. However, in one location, rye, and a mixture of cover crops decreased bulk density and increased available water content and organic matter. The poultry litter had a positive effect on most soil physical and chemical health indicators. The cover crop species at Pontotoc decreased bulk density, increased field capacity, CEC, and total carbon. However, there was no significant effect of cover crops on most soil chemical health indicators, and soil responses may take more than five years for the changes to appear.