Addenda held in pocket pasted onto back end paper. / Bibliography: leaves 137-165. / xvi, 165 leaves : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis involves the retrospective investigation of the insulin-like growth factors and their binding proteins in the serum of children with chronic renal failure (CRF) and transplantation, before and after treatment with recombinant human growth hormone (rhGH). IGF-IGFBP complexes in pooled serum from prepubertal and pubertal children of both sexes with CRF and renal transplantation, before and after treatment with rhGH, are analysed by fast protein liquid chromatography under neutral conditions. / Thesis (M.D.)--University of Adelaide, Dept. of Paediatrics, 1997?

1 v. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1994

Thesis (Ph. D.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.

Recombinant Bovine Somatotropin (rBST) is a biotechnology for increasing milk production in dairy cattle. The purpose of this research was to investigate and to build a better understanding of the complexities and controversies around this product in Canada. To accomplish this, I examined the Standing Senate Committee on Agriculture and Forestrys inquiry into rBST and the drug approval process. I compared and contrasted the testimony of witnesses and Senators and I uncovered emerging issues, patterns, and themes. This research was an exploratory and qualitative exercise that analyzed how the participants of this Senate inquiry conceptualized and contested the meaning of science, safety, and the states regulatory functions. <p> This research revealed several commonalities between Health Canada management, the human safety panel, and industry representatives. These witnesses argued that the drug approval process must be efficient, standard-driven, and based upon available scientific studies. These witnesses stated that they had confidence in the neutrality and competency of internal standard setting-agencies. They emphasized transparency rather than public participation in the drug approval process. Health and safety were conceptualized as static phenomena to be measured and evaluated by experts. <p>In contrast, Health Canada employees had several commonalities with the Senators, dairy representatives, and witnesses from citizen interest groups. Their testimony supports the argument that health and safety are dynamic social constructs. These actors transformed the boundaries of science to accommodate their precautionary framing of safety. They highlighted several problems with Canadas science-based regulatory framework and demanded that they have a decisive voice in the rBST decision. They challenged the hegemony of industrial capitalism by combining both scientific and lay knowledge to expose the limits and contradictions of industrialized agriculture.

Recombinant Bovine Somatotropin (rBST) is a biotechnology for increasing milk production in dairy cattle. The purpose of this research was to investigate and to build a better understanding of the complexities and controversies around this product in Canada. To accomplish this, I examined the Standing Senate Committee on Agriculture and Forestrys inquiry into rBST and the drug approval process. I compared and contrasted the testimony of witnesses and Senators and I uncovered emerging issues, patterns, and themes. This research was an exploratory and qualitative exercise that analyzed how the participants of this Senate inquiry conceptualized and contested the meaning of science, safety, and the states regulatory functions. <p> This research revealed several commonalities between Health Canada management, the human safety panel, and industry representatives. These witnesses argued that the drug approval process must be efficient, standard-driven, and based upon available scientific studies. These witnesses stated that they had confidence in the neutrality and competency of internal standard setting-agencies. They emphasized transparency rather than public participation in the drug approval process. Health and safety were conceptualized as static phenomena to be measured and evaluated by experts. <p>In contrast, Health Canada employees had several commonalities with the Senators, dairy representatives, and witnesses from citizen interest groups. Their testimony supports the argument that health and safety are dynamic social constructs. These actors transformed the boundaries of science to accommodate their precautionary framing of safety. They highlighted several problems with Canadas science-based regulatory framework and demanded that they have a decisive voice in the rBST decision. They challenged the hegemony of industrial capitalism by combining both scientific and lay knowledge to expose the limits and contradictions of industrialized agriculture.

published_or_final_version / abstract / toc / Zoology / Master / Master of Philosophy

The effects of insulin on milk production were tested employing two different approaches. Firstly, 12 Holstein cows were used to determine the effects of feeding calcium propionate (Ca prop) on dry matter intake (DMI) and production traits. The experimental design was a switchback with 2 treatments (Ca prop at 0 or 300 g/d). The DMI was lower when animals received Ca prop. Ca prop did not affect the yield of milk and its components; however, Ca prop increased protein content. The (acetate+butyrate)/propionate ratio in rumen fluid 2 h after feeding was lower when cows received Ca prop. Plasma insulin concentration was not different between treatments and the putative effect of propionate as an insulin secretagogue was probably related to the maintenance of insulin levels when DMI was lower. In conclusion, Ca prop is a potential feed ingredient to increase protein content in milk. The second approach consisted of intravenous infusion of insulin. A trial was designed to test the effects of insulin, recombinant bovine somatotropin (rbST) and their interaction in lactating dairy cows. Eight Holstein cows were used in a Latin Square design with 4 treatments: (1) intravenous infusion of saline, (2) infusion of saline and administration of 40 mg of rbST per day, (3) intravenous infusion of 12 mg of insulin per day coupled with glucose infusion and (4) rbST administration combined with insulin and glucose infusion. The theory that rbST causes a peripheral resistance to insulin was confirmed. Insulin infusion increased percent protein, percent casein and decreased milk urea content regardless of rbST administration. For milk yield, protein yield, casein yield, lactose percent and lactose yield, there was an interaction between insulin and rbST administration. Similarly, there was an interaction between insulin and rbST on plasma IGF-I levels. Fat yield was higher, with a higher content of long chain fatty acids, during rbST administration, regardless of insulin infusion. I

No description available.

The main purpose of this study was to investigate the relationship between human growth hormone (hGH) and two separate components of resistance exercise. Eight non-weight-trained subjects (23.33 ± 0.3 yrs) performed three force production trials (FPT), at different concentric workloads, and an 120% eccentric exercise trial (EET) on the Cybex 6000. Blood samples (3 mls) were taken pre- and post-exercise and analyzed for lactate, creatine kinase (CK) and hGH. Electromyographic (EMG) activity of the quadriceps muscle was recorded during each trial. The mean peak torque produced during the FPTs increased as work intensity increased but was the highest in the EET. The data for total work showed a proportional relationship with the intensity of the three concentric work loads but not the 120% EET. EMG activity of vastus medialis (VM) and rectus femoris (RF) measured during EET was 26% less than RF of 50% and 15% less than VL of 70% in FPT, respectively. The highest hormonal response occurred following the 120% EET. The hormonal response following the FPTs was highest in the 90% FPT with the two lower work intensity trials (50 and 70%) showing no clear hormonal response. Although the hGH response was the highest in the 120% EET, the post-exercise lactic acid levels in EET were 24% less than that of the 90% FPT. Creatine kinase (CK) activity was significantly elevated 36 hours after the last bout of EET which suggests that the eccentric exercise resulted in muscle damage. The results from concentric trials showed that muscle force generation, EMG, and lactic acid of the three different concentric trials were well correlated to the pattern of hGH secretion. However, only peak torque was consistent with the hGH response of the EET. The highest peak torque and hGH levels were achieved with eccentric exercise. The highest levels of fatigue, as a result of the combination of longer exercise time and overloading of the muscle during EET, may explain the higher hGH output. The muscle damage caused by the eccentric trial was enough to induce delayed onset of muscle soreness and may be the stimulus for the higher hGH output. The hGH response may facilitate repair of the muscular damage induced by eccentric exercise by promoting protein synthesis. / School of Physical Education

Bibliography: leaves 148-189. / viii, 189 leaves, [5] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / A transgenic rat model was developed to allow the study of the effects of GH expression, growth regulation and reproductive function. Transgenic rats were created by pronuclear microinjection of a human metallothionein promoter porcine growth hormone (pGH) gene construct. / Thesis (Ph.D.)--University of Adelaide, Dept. of Obstetrics and Gynaecology, 1996?