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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Investiga??o da variabilidade gen?tica em bagres de interesse comercial e para conserva?

Moraes Neto, Americo 25 September 2009 (has links)
Made available in DSpace on 2017-07-21T19:59:49Z (GMT). No. of bitstreams: 1 Americo Moraes.pdf: 5820633 bytes, checksum: 965e01013ad416e0fcd25ab3ad7ec73d (MD5) Previous issue date: 2009-09-25 / Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná / Currently, studies of aspects of evolutionary biology in neotropical fishes diversity are still lacking, despite the strong environmental impact caused by human action that has had a negative effect in native species, many of which probably still unknown to science. The knowledge of genetic variability within and between populations is of utmost importance for planning of both fish breeding and conservation of natural populations. In the present study we have analyzed, especially through molecular cytogenetics techniques, 29 specimens of Pseudoplatystoma reticulatum (Paraguay river, state of Mato Grosso do Sul), 14 specimens of Pimelodus britskii (Igua?u river, state of Paran?), 6 specimens of Sorubim lima (Paraguay river, state of Mato Grosso do Sul) and 10 specimens of Steindachneridion parahybae (Para?ba do Sul river, state of S?o Paulo). Results indicate 2n = 56 chromosomes for all species, a distinct karyotype formula composed mainly of biarmed chromosomes. Other particular features were found, regarding the location for Ag+NORs and fluorescent in situ hybridization with 5S and 18S probes. Except P. britskii, which showed NORs in the terminal region of the long arm, all species showed Ag+NORs in the terminal position of the short arm. Another peculiar feature of this fish was the co-localization of one of the 5S and 18S rDNA sites, which could be considered an apomorphy to the others Pimelodidae studied. The present work aims to contribute to the collection of data on the cytogenetics studies of Siluriformes, considering that much of the here presented information are the first characterization of the karyotype macro-struture of analysed fishes. / Considerando-se a diversidade ictiofaun?stica neotropical, estudos sobre aspectos de biologia evolutiva s?o ainda pouco expressivos. Em contraste, o forte impacto ambiental causado pela a??o humana tem refletido negativamente sobre as esp?cies nativas, muitas ainda desconhecidas da ci?ncia. O conhecimento da variabilidade gen?tica intra e inter populacional ? de extrema import?ncia para o planejamento tanto da piscicultura quanto para a conserva??o das popula??es naturais. No presente trabalho foram analisados citogeneticamente, sobretudo aplicando t?cnicas de citogen?tica molecular, 29 exemplares de Pseudoplatystoma reticulatum (rio Paraguai, MS), 14 esp?cimes de Pimelodus britskii (rio Igua?u, PR), 6 indiv?duos de Sorubim lima (rio Paraguai, MS), e 10 exemplares de Steindachneridion parahybae (rio Para?ba do Sul, SP). Os dados obtidos revelaram 2n = 56 cromossomos para todas as esp?cies analisadas, f?rmulas cariot?picas distintas, compostas basicamente de cromossomos de dois bra?os e caracter?sticas pr?prias em rela??o ? localiza??o das RONs atrav?s da impregna??o pelo ?on Ag+ e pela hibrida??o fluorescente in situ com sondas de DNAr 5S e 18S. Com exce??o de P. britskii, que apresentou RONs na regi?o terminal do bra?o longo, todas as outras esp?cies apresentaram marca??es das Ag+RONs em posi??o terminal no bra?o curto. Outra caracter?stica peculiar de P. britskii foi a localiza??o sint?nica de um dos s?tios de DNAr 5S e o 18S em cromossomos subteloc?ntricos, atributo que pode ser considerado uma apomorfia em rela??o a outros pimelod?deos estudados. O presente estudo visa contribuir com o acervo de dados citogen?ticos a respeito dos Siluriformes em estudo, considerandose que muitas das informa??es correntes tratam-se das primeiras caracteriza??es da macroestrutura cariot?pica das esp?cies de peixes analisadas.

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