This thesis examines the contribution of a non-indigenous red alga, Mazzaella japonica, to wrack subsidies in Baynes Sound, British Columbia, and the effects of its removal by a commercial beach-cast harvest. Field and laboratory work was conducted to determine: 1) How large wrack inputs are in terms of biomass and spatial extent within the harvest region, and what proportion of this is comprised of M. japonica; 2) how wrack characteristics influence associated macrofauna communities; 3) if there is any detectable effect of beach-cast harvesting on either the wrack characteristics or macrofauna communities; and 4) if M. japonica provides a food source for native invertebrate consumers within the subtidal and supralittoral zones. Field surveys conducted from November 2014 through March 2015 found that wrack biomass within the harvest region could reach as much as 853 kg (±173 SD) per meter of shoreline, and cover up to 35 m2 (± 3 SD) of beach surface within this area. The macrophyte composition of the wrack was dominated by M. japonica, which accounted for 90% of the identifiable macrophyte biomass on average. Wrack in the later stages of decomposition hosted the most speciose and diverse assemblages of macrofauna, though community composition also differed among collection sites and with depth of the wrack. Though we were limited in our ability to disentangle the effects of beach-cast harvesting due to a concentration of effort at one site, we failed to detect any large influence on wrack biomass or macrofauna communities. Harvesting does, however, appear to be associated with a greater area of wrack cover and decreased mean depth. Stable isotope mixing models estimated that M. japonica contributed no more than 22% and 17% on average to the diets of supralittoral and subtidal consumers respectively, despite its overwhelming dominance in both environments. These results suggest that the non-indigenous red alga may experience a reprieve from herbivory within the subtidal environment. A lack of consumption within the supralittoral zone could influence nutrient cycling on recipient beaches and increase propagule pressure in the surrounding regions. Results from these studies are intended to help inform the management of M. japonica and its commercial harvesting. Combined, they indicate that this non-indigenous seaweed does not provide a substantial subsidy in the form of food provision for resident invertebrates. Furthermore, the commercial removal of M. japonica is small compared to the total biomass available, and had no detectable effect on the wrack-associated macrofauna communities examined. / Graduate / 2017-08-19 / jjulin.holden@gmail.com

Ecosystems are linked by spatial subsidies, the bi-directional flows of nutrients, materials and energy that cross ecosystem boundaries. Considered one of the planet’s most productive and diverse meta-ecosystems, the broad interface between land and sea is crossed by innumerable abiotic and biotic spatial subsidies, including migratory animals. Routinely crossing ecological boundaries, migrants play significant roles in subsidizing receiving ecosystems, including influencing ecosystem productivity, diversity, community structure and trophic cascades. On the Pacific coast of North America, spatial subsidies driven by migratory Pacific salmon have been intensively studied. Like many of the world’s migrants, however, salmon populations have declined considerably and most of our scientific knowledge has been gained from a diminished subsidy. Other subsidies, including those driven by migratory species in decline, remain relatively unknown. Each year, Pacific herring (Clupea pallasii) migrate to shallow waters to spawn on nearshore and intertidal substrates. Despite suggestions in the literature that herring, an abundant, nearshore/intertidal spawning forage fish, subsidizes coastal ecosystems, there had been no investigation of cross-ecosystem interactions. Just as stable isotopes and fatty acids have been used to explore wrack (drift macrophytes) subsidies to intertidal ecosystems, we combined both approaches to trace the input of Pacific herring and wrack to semi-terrestrial amphipods (Traskorchestia spp.), which are highly abundant detritivores in beach ecosystems. Brown algae and seagrass were major contributors to amphipods but when available, herring was also a significant resource. Because amphipods are prey for terrestrial consumers, including bears (Ursus spp.), we also identified indirect trophic linkages between herring and terrestrial ecosystems. Bears are major consumers and vectors of salmon into terrestrial ecosystems, but little is known regarding their involvement in other spatial subsidies. Using a model-based inference approach paired with remote cameras to monitor intertidal black bear (U. americanus) activity, we determined that the best predictors of black bear intertidal activity were major intertidal prey items (herring and amphipod biomass) and Julian day. Bears positively responded to herring and amphipod biomass on beaches but it was the analysis of scats that determined the contribution of herring eggs to the diets of bears. In 2010, the herring spawn was relatively poor and consumption of eggs was negligible, with amphipods constituting a major portion of bear diets. In following years, herring egg loading was relatively high and eggs were the dominant dietary item in bear scats. Tracing the contribution of herring into terrestrial areas proved challenging and instead, we furthered knowledge of the within-watershed spatiotemporal influences of salmon on conifer tree ring growth and δ15N signatures. Both tree ring growth and δ15N signatures tracked the known spatial distribution of salmon carcasses. Using a model-based inference approach, salmon abundance and interaction terms of salmon*temperature and salmon*distance into the forest best predicted tree growth. In contrast, salmon abundance was not a leading predictor of δ15N. By broadening our understanding of the fine-scale influence of salmon on a stand of ancient trees, this research is expected to contribute to future exploration of the terrestrial influences of Pacific herring. / Graduate / 0329 / cfox@uvic.ca