Thesis (M.S.)--Oregon State University, 2009. / Printout. Includes bibliographical references (leaves 59-67). Also available on the World Wide Web.

Thesis (Ph.D.)--Montana State University--Bozeman, 2007. / Typescript. Chairperson, Graduate Committee: Andrew Hansen. Includes bibliographical references.

Thesis (M.S.)--Montana State University--Bozeman, 2007. / Typescript. Chairperson, Graduate Committee: Clayton B. Marlow. Includes bibliographical references (leaves 48-52).

Oomycetes are eukaryotic fungus-like microorganisms that are known to be pathogenic to plants and animals, causing both ecological and economic damage. Animal pathogenic oomycetes, such as Saprolegnia species are destructive pathogens to many aquatic organisms and are found in most parts of the world. Phytophthora species cause a number of plant diseases. Pythium are less understood as many of these species are saprotrophs which are not thought to be pathogenic. Oomycete research has focussed mainly towards the characterisation and control of pathogenic species with very little information on the ecology of Oomycetes. In this thesis, oomycete species were isolated from water samples collected from rivers in Scotland and Northern England. Baiting and water filtration were used to compare the reliability of both methods, and nested PCR was used to compare the number and types of species obtained in comparison to conventional culturing methodologies. The results suggest that water filtration provided a fast, reliable method for isolating abundant, hardier species such as Pythium undulatum, Saprolegnia diclina, Saprolegnia delica, and Saprolegnia ferax. Water chemistry, surrounding land use, seasonality and land elevation were found to be important factors in Oomycete diversity. Nested PCR of oomycetes directly from filters to obtain target DNA from organisms which are more difficult to culture or less numerous proved to be useful for some species, but will need more refinement of primer and methodologies to obtain species of interest. Sampling of the rivers resulted in the isolation of three new Pythium spp. isolated from the Rivers Spey and Dochart in Scotland. Phylogenetic analysis, infection studies, growth rates and microscopy were used to characterise these three species.

Genus Trichoderma - anamorph of the genus Hypocrea (Hypocreaceae, Hypocreales, Sordariomycetes, Ascomycota) belongs to saprotrophic filamentous micromycetes. The representatives of this genus are known primarily as cosmopolitan inhabitants of soil ecosystems, including leaf litter and woody debris. This work summarizes the results of the study of isolates belonging to the genus Trichoderma originating from the territory of the Czech Republic, mainly from soil and leaf litter. During the study, altogether 267 isolates were analysed. For all of the isolates the following methods were used: phenotypic characterisation, UP-PCR fingerprinting, gene sequencing in tef 1α and ITS regions, and in the strain AK 115/00 also in rpb2 region. The main result of the study was the identification of 21 species of genus Trichoderma belonging into 4 groups and discovery of one isolate of species yet unknown to science. Species T. crassum, T. gamsii, T. ghanense, T. rossicum and T. longipile were found in the Czech Republic for the first time. According to the literature review and results of this study, 34 different Trichoderma species is currently known from the Czech Republic. In the work there is also discussed low reliability of identification of certain species based only on phenotypic characters. Key words:...

京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第20214号 / 理博第4299号 / 新制||理||1617(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)准教授 川北 篤, 教授 田村 実, 准教授 酒井 章子 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第20772号 / 農博第2255号 / 新制||農||1055(附属図書館) / 学位論文||H29||N5092(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 德地 直子, 教授 神﨑 護, 教授 吉岡 崇仁 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

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京都大学 / 新制・課程博士 / 博士(理学) / 甲第24459号 / 理博第4958号 / 新制||理||1708(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 本川 雅治, 准教授 中野 隆文, 教授 中務 真人 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM