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SPECTROSCOPIC STUDIES OF NUCLEAR SPINS POLARIZED VIA SPIN EXCHANGE OPTICAL PUMPING AND DYNAMIC COUPLING IN CRYPTOPHANE HOST-GUEST COMPLEXESNikolaou, Panayiotis 01 December 2010 (has links)
NMR is a powerful analytical spectroscopic tool used to perform detailed studies of structure and dynamics of molecules in solution. However, despite NMR's excellent spectral sensitivity, most NMR methods suffer from low detection sensitivity. This low detection sensitivity results largely from extremely small (Boltzmann) nuclear spin polarization at thermal equilibrium--in even the strongest of magnets. This dissertation focuses on selected research areas that maybe used to combat the limitations presented by NMR and measure weak spectral responses with atomic-scale precision. In particular, these methods involve the use of laser-polarized xenon, liquid crystals, and polarization transfer (cross-polarization) techniques to enhance NMR sensitivity and/or measure weak interactions. The potential use of these tools to study host-guest interactions is of particular interest. In certain systems the sensitivity problem of conventional NMR/MRI can be overcome by applying optical pumping (OP) methods to enhance nuclear spin polarization. For instance, OP of noble gases (such as xenon) is employed to dramatically increase their nuclear spin polarization by transferring angular momentum of laser light to electronic and then nuclear spins. Next, cryptophane complexes are ideal choices for fundamental studies of prototypical host-guest interactions. Of general interest when studying host-guest interactions is how (1) physical confinement at the nanoscale and (2) interactions between guest and host may affect the properties, dynamics, interactions, and/or reactivity of a trapped molecule and the host/guest complex as a whole. As a more specific example, we are interested in probing host-guest dynamic coupling, which refers to the relative motion of the guest within the host, determined by the relative sizes and geometries--as well as the interactions involved. With the development of new NMR methods and techniques, we hope to gain insight into mechanisms that underlie complex formation by probing the structures, dynamics and energetic contributions involved in ligand binding, where molecular contributions such as: orientational and motional freedom of the guest; and structure, dynamics, and ordering of the host can influence the behavior of inclusion complexes.
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Using High-Powered, Frequency-Narrowed Lasers For Rb/129Xe and Cs/129Xe Spin-Exchange Optical Pumping To Achieve Improved Production of Highly Spin-Polarized Xenon For Use In Magnetic Resonance ApplicationsWhiting, Nicholas 01 December 2010 (has links)
Nuclear magnetic resonance (NMR) spectroscopy has been extensively used to investigate numerous systems of interest, ranging from collections of molecules to living organisms. However, NMR suffers from one key drawback: an inherent lack of detection sensitivity, as compared to other common forms of spectroscopy. This is due to the minute nuclear magnetic moments and low nuclear spin polarization levels at thermal equilibrium (~10-5 to 10-6), and thus necessitates the use of relatively large sample volumes. One way to overcome this low detection sensitivity is to introduce a species with highly non-equilibrium nuclear spin polarization, such as `hyperpolarized' xenon-129. Hyperpolarized xenon can either be used as its own chemical sensor (due to its exquisitely sensitive chemical shift range), or the non-equilibrium polarization may be transferred from xenon to another molecule of interest (such as a protein or inclusion complex). Hyperpolarized xenon is produced through a process known as spin-exchange optical pumping (SEOP), where the angular momentum from resonant, circularly-polarized light is transferred to the electronic spins of an alkali-metal, and is subsequently transferred to the xenon nuclei through gas-phase collisions. While SEOP has been extensively characterized throughout the years, new experimental techniques and emerging technologies have considerably advanced the field in recent years, and may enable a new understanding of the underlying physics of the system. The first five chapters in this dissertation review background information and the principal motivations for this work. Chapter one reviews the basics of NMR, from the various components of the nuclear spin Hamiltonian and different spin-relaxation pathways to the reasons behind the low polarization of nuclear spins at thermal equilibrium and a few alternative methods to `boost' the NMR signal. Chapter two discusses the fundamental aspects of SEOP, including the electronic spin polarization of the alkali-metal, polarization transfer to the xenon nuclei, and different avenues for the spin polarization to be depleted. The third chapter covers the practical considerations of SEOP from the viewpoint of an experimentalist; namely, the experimental differences when using a variety of alkali metals and noble gases, as well as different SEOP apparatuses and experimental parameters. Chapter four details a variety of different light sources that may be used for SEOP; specifically, the use of laser diode arrays (LDAs) are reviewed, including LDAs that have been frequency-narrowed for more efficient light absorption by the alkali metal. The fifth background chapter covers a variety of magnetic resonance applications of hyperpolarized xenon, including molecular biosensors, specific and non-specific binding with proteins, materials studies, and in vivo applications. The sixth chapter is used as an overview of the dissertation research, which is presented in chapters seven through eleven. Chapter seven details the arrangement of the particular SEOP apparatus used in this research, as well as the experimental protocol for producing hyperpolarized xenon. The eighth chapter accounts the implementation and characterization of the first frequency-narrowed LDA used in this research, as well as an equal comparison to a traditional broadband LDA. Chapter nine introduces the use of in situ low-field NMR polarimetry, which was used to distinguish an anomalous dependence of the optimal OP cell temperature on the in-cell xenon density; the low-field set-up is also used to examine the build-up of nuclear spin polarization in the OP cell as it occurs. The tenth chapter covers the use of high power, frequency-narrowed light sources that are spectrally tunable independent of laser power; this allows for the study of changes to the optimal spectral offset as a function of in-cell xenon density, OP cell temperature, and laser power. Xenon polarization build-up curves are also studied to determine if the spectral offset of the laser affects the nuclear spin polarization dynamics within the OP cell. Finally, chapter eleven accounts the use of high power, broadband LDAs to perform SEOP in which cesium is used as the alkali metal; these results demonstrate (for the first time) that the xenon polarization generated by cesium optical pumping can surpass that of rubidium OP under conditions of high laser flux and elevated in-cell xenon densities.
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The Efficiency Limits of Spin Exchange Optical Pumping Methods of 129Xe Hyperpolarization: Implications for in vivo MRI ApplicationsFreeman, Matthew S. January 2015 (has links)
<p>Since the inception of hyperpolarized 129Xe MRI, the field has yearned for more efficient production of more highly polarized 129Xe. For nearly all polarizers built to date, both peak 129Xe polarization and production rate fall far below theoretical predictions. This thesis sought to develop a fundamental understanding of why the observed performance of large-scale 129Xe hyperpolarization lagged so badly behind theoretical predictions.</p><p> This is done by thoroughly characterizing a high-volume, continuous-flow polarizer using optical cells having three different internal volumes, and employing two different laser sources. For each of these 6 combinations, 129Xe polarization was carefully measured as a function of production rate across a range of laser absorption levels. The resultant peak polarizations were consistently a factor of 2-3 lower than predicted across a range of absorption levels, and scaling of production rates deviated badly from predictions based on spin exchange efficiency.</p><p> To bridge this gap, we propose that paramagnetic, activated Rb clusters form during spin exchange optical pumping (SEOP), and depolarize Rb and 129Xe, while unproductively scattering optical pumping light. When a model was built that incorporated the effects of clusters, its predictions matched observations for both polarization and production rate for all 6 systems studied. This permits us to place a limit on cluster number density of <2 × 109 cm-3.</p><p> The work culminates with deploying this framework to identify methods to improve polarization to above 50%, leaving the SEOP cell. Combined with additional methods of preserving polarization, the polarization of a 300-mL batch of 129Xe increased from an average of 9%, before this work began, to a recent value of 34%.</p><p> We anticipate that these developments will lay the groundwork for continued advancement and scaling up of SEOP-based hyperpolarization methods that may one day permit real-time, on-demand 129Xe MRI to become a reality.</p> / Dissertation
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Magnetization dynamics in paramagnetic systemsRantaharju, J. (Jyrki) 07 December 2018 (has links)
Abstract
This thesis reports simulations of direct observables in electron and nuclear spin relaxation experiments in an example paramagnetic system, as well as polarization transfer occurring in a spin-exchange optical pumping (SEOP) experiment. Studies of paramagnetic relaxation are important, e.g., in the development of agents used for enhanced contrast in magnetic resonance imaging. SEOP is used to produce hyperpolarized noble gases, which are then used to, e.g., enhance sensitivity in structural studies of matter with nuclear magnetic resonance. Presently the theory, available software and hardware for such computational modeling have reached a state in which quantitative reproduction of the experimentally observed magnetization decay is possible from first principles.
The present multiscale computations are carried out from first principles combining molecular dynamics simulations of atomistic motion and quantum-chemical electronic structure calculations of the spin interaction parameters that enter the effective spin Hamiltonian. A time series of the spin Hamiltonian is then explicitly used to propagate spin dynamics in the system, and dynamical time constants of the magnetization are obtained through ensemble averaging. The complete decay of electron spin magnetization could be followed directly within the duration of the simulation, whereas the nuclear spin relaxation rates were extracted using Kubo’s theory regarding generalized cumulant expansion and stochastic processes.
The extracted electron and nuclear spin relaxation rates for the chosen prototypic system, the aqueous solution of Ni²⁺, are in quantitative and semi-quantitative agreement, respectively, with the available experimental results. The simulations of polarization transfer corroborate the empirical observations on the importance of van der Waals complexes and binary collisions in the spin-exchange process. Long van der Waals complexes represent the overwhelmingly most significant kind of individual events, but the short binary collisions can also give a relatively important contribution due to their vast abundance. This thesis represents a first study in which first principles-calculated trajectories of individual events could be followed.
The simulations reported in this thesis were run without any empirical parametrization and thus represent a significant step in first-principles computational modeling of magnetization dynamics.
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