The purpose of this study is to investigate the use of isometric mid-thigh pulls to determine lower-limb asymmetrical strength differences in NCAA D-I athletes. Sixty-six subjects (40 males and 26 females) performed 2 maximal effort isometric pulls over two force plates sampling at 1000 Hz each. Peak force was scaled for body weight, and rate of force development was examined from 0-200ms. Results of the study show subjects' produced significantly greater scaled force with the left leg as compared to the right leg; however, no significant differences existed for rate of force development (RFD). Men exhibited significant differences between both scaled peak force and RFD, while women only showed significant differences in scaled peak force. Of the 66 subjects tested, 6 subjects (5 men and 1 woman) exhibited percent differences of 15% or greater asymmetry for scaled peak force. The results indicate that isometric mid-thigh pulls are a way to show the presence of asymmetries in D-I athletes.