Inland waters such as streams and lakes have recently been found to be supersaturated with both carbon dioxide (CO2) and methane (CH4) – the high concentrations resulting in significant natural emissions of greenhouse gases (GHGs). Previous studies have shown that streams emit particularly large amounts of GHGs per area covered, but the spatial variability is very high and has rarely been studied in detail. This study focuses on the variability of aquatic CO2 and CH4 concentrations with high spatial resolution in a hemi-boreal stream. The study area is a 7 km2 catchment in Skogaryd in southwest Sweden. 131 samples were collected and the stream was divided into groups depending on slope gradient and geographical placement. The results show that the concentrations had high spatial variability, especially regarding CH4, and that the concentrations are higher and more variable at lower slope gradients, which possibly indicates an increased gas exchange at higher slopes. The results also showed that concentrations can increase or decrease sharply over short distances in relation to changing slope gradient. This shows that frequent spatial sampling is needed to more accurately represent streams than what is often the case in many studies. A general distance between sampling locations could not be found due to the high variability of concentrations. Instead, the authors suggest that future studies of CO2 and CH4 concentrations in streams use a stratified random sampling strategy based on slope gradients.