This study is the first to consider how the combination of root physical effects, microbial production of EPS, and root effects on the hydrodynamic boundary layer could influence streambank soil erodibility. Specifically, the goal of this research was to quantify the physical and biological effects of roots on streambank fluvial erosion. A series of laboratory-scale erosion tests were conducted using a mini jet erosion testing device and a recirculating flume channel to address this goal. Several soil and vegetation factors that influence fluvial entrainment, like extracellular polymeric substances (EPS), soil aggregate stability and root length density, were measured following erosion testing. For flume experiments, three streambank boundary conditions were constructed to simulate unvegetated streambanks, as well as streambanks with herbaceous and woody roots. Soil treatments were also created to represent unamended and organic matter (OM) amended soil either without roots (bare soil), with synthetic roots, or with living roots (Panicum virgatum). Median soil erosion rates along the simulated rooted boundaries were two to ten times higher compared to the unvegetated boundary due to protruding root impacts on the boundary layer. In flume experiments, median erosion rates were 30% to 72% lower for unamended soils containing compacted synthetic root fibers as compared to bare soil samples. Adding both OM and fibers to the soil had a greater effect; the median erosion rate reductions of live rooted treatments (95% to 100%) and synthetic rooted + OM treatments (86% to 100%) were similar and statistically lower than bare soil controls. Stimulated microbial production of EPS proteins were significantly correlated with increased erosion resistance in OM-amended treatments while OM treatments had significantly lower EPS carbohydrates compared to unamended treatments. In summary, while sparsely spaced roots exposed on streambanks may increase soil erosion rates due to impacts on the hydrodynamic boundary layer, overall results highlight how the synergistic relationship between root fibers and soil microbes can significantly reduce streambank soil erodibility due to fiber reinforcement and EPS production. / Doctor of Philosophy / Plant roots are known to protect streambank soils from erosion by water; however, exactly how roots provide this protection has remained unclear. Among other things, roots can influence streambank soil erosion by holding soil together through a thick root network, interacting with soil microorganisms to stimulate the release of "sticky" organic compounds called extracellular polymeric substances (EPS), and altering the force of the water against the streambank. This research aimed to quantify and compare the relative importance of these three mechanisms on streambank soil erosion using a mini jet erosion testing device and an indoor recirculating flume channel. To do this in the flume, three walls were constructed to simulate unvegetated streambanks, as well as streambanks with herbaceous and woody roots. In greenhouse settings, soil treatments were also created to represent unamended and organic matter (OM) amended soil either without roots (bare soil), with artificial roots, or with living roots (Panicum virgatum). While roots protruding out of streambanks appeared to increase median soil erosion rates due to the impact of roots on near-bank flow, artificial roots in the soil and OM amended soils reduced soil erosion rates. Specifically, OM amendments stimulated the production of EPS proteins, leading to improved soil stability and increased soil resistance to erosion by water. Overall results highlight how the synergistic relationship between root fibers (living roots and artificial roots) and soil microbes can significantly reduce streambank soil erodibility due to root binding and microbial production of EPS. While plant roots naturally provide both fibers and EPS to soils, these materials could be incorporated into fill soils during construction to rapidly increase soil erosion resistance following levee construction and stream restoration projects.