This study examined the relationship of life stress, competitive trait anxiety and competitive state anxiety, with injury occurrence in NCAA Division I athletic teams. Life stress was measured by the Life Events Survey for Collegiate Athletes. The Competitive State Anxiety Test and Marten’s Sport Competition Trait Anxiety Test calculated competitive anxiety. After calculating means and standard deviations for each measure, a multiple regression was run. A two-way ANOVA was used to determine differences between sport and gender. The results of the study indicated there was no effect of gender for trait anxiety, cognitive and somatic state anxiety or gender. A significant main effect was found between gender and self-confidence. There was no effect of gender for life stress. There was no effect of sport for cognitive state anxiety, somatic state anxiety, self-confidence, or life stress. The CSAI-2 self-confidence showed no significant difference between sport. No significant relationships were noted between injury score and any of the independent variables.