The potential of surfactants for improving the yields of alpha-amylase during the solid substrate fermentation (SSF) of potato starch using pure and mixed cultures was examined. The microorganisms used in this study were Aspergillus oryzae ATCC 1011, Bacillus subtilis ATCC 21556 and Bacillus subtilis ATCC 21332. The surfactants tested were Tween 20, Tween 80, SDS and surfactin. The fermentations were carried out in perforated trays after the addition of 10% (v/w) inoculum and with temperature and humidity controlled at 30°C and 90% RH respectively. Samples were taken and analyzed quantitatively for the production of alpha-amylase and biomass and qualitatively by scanning electron microscopy (SEM) using a JSM-840 A scanning microscope at 10 kV accelerating voltage. / It was possible to increase fungal alpha-amylase production by as much as 6 fold in the process with the addition of either synthetic surfactants or the biosurfactant surfactin. The bacterial alpha-amylase yields increased up to 11.5 fold in with the addition and/or the co-culture production of surfactants. The highest enzyme activity was found in the fermentation of a mixed culture of the two Bacillus strains with the addition of Tween 80. During the SSF with B. subtilis ATCC 21332 and ATCC 21556 as a mixed bacterial culture, there was also the production of surfactin in yields comparable to those obtained in a submerged fermentation. The biofilm formation as observed by SEM appeared to be associated with the presence of surfactants in the process and was not formed when no surfactants were present. The biofilm was observed as an entrapment of the bacteria in the substrate, resulting in improved access to the starch and higher production of alpha-amylase.

No description available.

Theoretical studies on the wetting of cylindrical solid surfaces with axisymmetric rugosities explicitly showed the presence of contact angle hysteresis, non-equilibrium jumps during contact line motion and the formation of composite surfaces. For physically rough surfaces with unidirectional randomness it was shown that hysteresis increases with decreasing roughness dimensions and with increasing standard deviation in the solid surface slope distribution. / The wetting of solid surfaces by liquids was investigated by a new tensiometric technique called capillarography. At large roughness wavelengths ((TURN)100 (mu)m) the contribution of concentric sawtooth-shaped regular roughness was found to be close to the values calculated theoretically. At smaller roughness wavelengths ((TURN)1 (mu)m) the wetting is complicated by intrinsic roughness and chemical heterogeneity. / High energy surfaces such as platinum and quartz exhibit a regular stick-jump behavior when wetted with water above a very low critical contact line velocity. This is caused by minute amounts of surface active impurities in the system. From the experimental data it can be deduced that surface active molecules diffuse onto and over the solid surface. / A relaxation of the contact angle was observed when an initially slow moving contact line was brought to a full stop. This is attributed to the slow establishment of a new equilibrium, possibly by surface capillary waves, involving metastable contact line positions separated by energy barriers. For receding contact angles, the main cause of the relaxation seems to be a stabilization of a liquid film left behind a receding contact line. / The observations made in this study contribute to a better understanding of the complicated nature of the wetting of solids.

The growing interest in the industrialization of construction process; promotes opportunities for automation. Automation brings improvement in quality and productivity, while reducing worker’s exposure to hazardous work environments. The integration of robotics in interior finishing works, such as sanding and painting of drywalls is a relatively new concept. Progressing to a stage where fully autonomous robots are used for interior finishing works requires intermediate steps; namely surface profiling. This thesis describes a theoretical concept of shadow profilometery to profile the surface of an installed drywall. A shadow was cast over the area under consideration, and the shadow profile was captured as a 2D image by a camera. Digital image processing techniques were utilized for identifying regions that deviate from a flat surface. The methodology discussed in this research, was tested on a virtual system, and the results were found to be encouraging. / Construction Engineering and Management

This thesis details electrical and physical measurements of pulsed laser deposition-applied thin film coatings of Alumina, Ceria, and Yttria-stabilized Zirconia (YSZ) on a LiNi0.5Mn0.3Co0.2O2 (NMC) cathode in a Lithium ion battery. Typical NMC cathodes exhibit problems such as decreased rate performance and an opportunity for increased capacity exists by raising operation voltage beyond the electrolyte stability window. Very thin (~10 nm) coatings of stable oxides provide a pathway to solve both problems. As well, the electrochemical impedance spectra of the uncoated and coated cells were measured after different numbers of cycles to reveal the property variation in the cathode. Further understanding of the mechanism of rate performance enhancement and chemical protection by thin oxide coatings will continue to improve battery capability and open up new applications. Ceria-coated Li-NMC cells show the best capacity and rate performance in battery testing. Through electrochemical impedance spectroscopy (EIS), the surface film resistance was found to remain stable or even drop slightly after repeated cycling at high voltage. CeO2 is proposed as a coating for Lithium ion battery cathodes owing to its high chemical stability and the demonstrated but not yet well understood electrical conductivity. Alumina-coated cathode shows comparable performance as that of the uncoated cell in the early stage of the test, but through the course of testing the rate capability and recoverable capacity is improved. This is possibly due to Al2O3?s well-known abilities as HF scavenger and chemically inert nature. YSZ-coated cathode performs worse than the uncoated ones in terms of capacity, rate capability, and EIS-related figures of merit. The reason for the poor performance is not yet known, and repeatability tests are under way to verify performance. High voltage cycling reveals no obvious difference in irreversible loss between the coated or uncoated cells. The reason for the lack of distinction could be the relatively small percentage of surface coating compared to the thick doctor-blade processed cathode layer.

Raw material accounts for a large proportion (approximately 75 percent) of a sawmill’s operating costs. However, about 15 percent of raw material ends up as low valued sawdust and planer shavings due to inaccurate cutting. Sizable financial benefits can be realized through maximizing conversion of raw material into valuable solid wood. Advanced process control in a sawmill can help achieve straighter cuts closer to final product dimensions and reduce loss of valuable raw material. A novel and practical method for enhanced process control in a sawmill is presented. A laser arrangement consisting of industrial point and line scanners is used to obtain a surface profile of the entire (two-dimensional) top and bottom surfaces of a lumber board. Each surface profile is independent of the other and free of data contamination caused by relative motions between the measured surface and sensors. Point scanners and line scanners simultaneously record 1-D and 2-D height data, respectively, along the length of the board. One-dimensional height data are used to identify relative motions through a mathematical technique based on linear inverse theory. Subtracting relative motion information from raw line scanner data provides an accurate 2-D surface profile. A second line scanner placed below the board can be used to obtain a separate 2-D profile of the bottom lumber surface. Separate-sided profiling is advantageous because typically a different saw or machine mills each side of a board. Thus, knowing the surface profile of each side of a board is crucial not only in diagnosing a deficiency in the milling process but also in determining the location of this deficiency. Results demonstrate that two-dimensional surface profiling can identify common surface defects such as step, washboard and knot tear-out with an accuracy of 0.3mm. Reproduction of each surface is rapid (approximately 0.2 seconds) and stable.

A systematic research study was carried out in order to characterize the rheology of concentrated slurries prepared from eight nickel laterites. The experiments were carried out using a rotational viscometer, and the behavior of the laterites was evaluated in terms of the apparent viscosity and yield stress obtained through flow curve modeling. An attempt was made to correlate the results obtained for the laterite samples with data obtained for model single mineral systems as well as for model mixed mineral systems. In combination with detailed mineralogical characterization of the laterite samples, all the rheological results allowed a rheology-based laterite classification system to be proposed. Accordingly, the laterite samples gave the following responses: the SAPSIL samples (high-quartz) generally producedl ow yield stress values, the SAPFE samples (high-iron) were characterized by intermediate to high yield stress values, while the SAP samples (saprolite) gave the highest yield stress values. Interestingly, these dominant rheological responses of laterites could actually be predicted based on rheological tests carried out on model mineral suspensions (particularly goethite and quartz). Since the rheology of fine mineral suspensions is largely determined by the surface properties (surface charge) of the particles, a series of electro-acoustic measurements were also performed on model minerals and laterite samples to analyze the surface charge characteristics of the tested samples. It was demonstrated that the current electro-acoustic theory developed for single mineral systems can readily be used for modeling the behavior of mixed mineral systems. The modeling and experimental data agreed exceptionally well when constituent minerals were of the same surface charge under given pH. Clear but rather small deviations between experiment and theory were observed under conditions when the minerals were oppositely charged. This observation strongly suggested that inter-particle aggregation was most likely responsible for the observed discrepancies. Overall, the results of this thesis show that laterite slurries exhibit a wide range of rheological responses due to highly variable mineralogy, differences in particle size distributions, and difference in the surface properties of the many constituent minerals. It also shows that the surface properties of the minerals relates to rheology.

This dissertation addresses the problem of scanning the geometry of real objects and building accurate computer models of those objects. We present a complete system which employs a structured light scanner to acquire 3D views of objects from multiple viewpoints. These multiple views, expressed in a sensor-oriented coordinate system, are then registered into a model-centred coordinate system, before being integrated into a single mesh describing the object's geometry. Line of sight constraints forbid any single view from capturing the entire surface of an object, so multiple scans must be performed. We have developed registration techniques which may register all of the views simultaneously, resulting in a globally optimal solution. Statistical error modeling of the sensor, and the use of these models in the registration process, forms a key part of the research. It is motiviated by the observation that all measurements are subject to some degree of random measurement error. The true values of these errors cannot be determined, however their statistical properties may be modeled. Our registration system utilises these error models to improve registration accuracy, and to allow the accuracy of the registration to be estimated. The resulting system is a flexible platform for 3D data capture and modeling. It may be used in conjunction with the structured light scanner, or 3D data acquired from any other source. We demonstrate this capability with models constructed from sources such as laser range finders and scanning touch probe systems. The contributions of this thesis are as follows: a novel stereo matching algorithm which permits the estimation of stereo disparity as well as the uncertainty in the disparity, development of a practical 3D vision sensor based on structured light techniques, two novel algorithms for performing simultaneous multiple view point set registration, while supporting individual point error models and estimating the uncertainty in the registration solution, a novel algorithm for efficiently solving the multiple view registration problem, and the implementation of a number of existing surface correspondence and reconstruction techniques, permitting the development of an integrated 3D vision system for capturing and modeling 3D objects.

In order to clarify the role that water plays in the photocatalytic process, changes in the IR and Raman spectra of P25 TiO₂ thin films were observed upon exposure to liquid water. Further investigation of these spectral changes via dehydration of thin films under nitrogen and oxygen of different humidities led to the observation of spectroscopic features that have been assigned to localised surface phonon modes. When the effect of UV irradiation on these features was investigated, a broad IR absorption due to transitions of electrons in shallow traps was detected under dry nitrogen but not under dry oxygen. Further investigation of the photocatalytic properties of P25 TiO₂ showed a complete removal of a stearic acid film. The final products have been tentatively assigned to a mixture of short chain carbonyl species and adsorbed carbonates as well as carbon dioxide and water. The IR spectrum of the fuel cell membrane material Nafion is complex and literature data varies in some of the assignments. The compound perfluoro(2-ethoxyethane)sulfonic acid was used as a model compound for the Nafion side chain resulting in a clearer assignment of the Nafion IR spectrum. In light of these new assignments changes induced in the region 1100-1300 cm⁻� by variation in humidity and ion exchange have been shown to be mainly the result of changes in the sulfonate asymmetric stretching modes. By flowing a series of solutions containing tetramethylammonium ions and perchlorate ions the surface charge characteristics of a Pt black film were determined in the pH range 2-12. There proved to be a weak positive charge below pH 4 and a weak negative charge above pH 9. Between these points there appeared to be no overall charge on the surface. When perfluoro(2-ethoxyethane)sulfonic acid was adsorbed to a Pt black film changes in its IR spectrum indicated a strong binding via interactions between the sulfonate groups and the Pt surface. The nature of the adsorption of Nafion was less clear cut and, whilst adsorption is strong, it seems possible that hydrophobic interactions between the Nafion backbone and the surface are involved.

Conventional techniques for contact angle measurements do not perform well for small particles. The equilibrium capillary pressure technique (ECP) consists in measuring the pressure required to prevent liquid penetration into a packed bed of particles and calculating the contact angle from a simple model, namely the equilavent capillary model. The ECP is well suited for the measurement of advancing contact angles. In its most convenient version the capillary pressure is measured for two different liquids (one of which is fully wetting and thus allows the calculation of the effective capillary radius). The use ECP to obtain the receding contact angle on powders has been developed. The major difference bertween a liquid penetrating a porous bed and retreating from it is that liquid pockets are left behind in the receding case. Effectively, this reduces the porosity of the packed bed. The volume fraction of the retained liquid apparently depends on the surface tension of the liquid but is only marginally affected by the wettability and size fraction of the particles. Therefore a simple procedure for the determination of the receding contact angle, based on the use of a calibrating liquid, is outlined and verified. The approach gives realistic values for the receding contact angle. / Thesis (PhDApSc(MineralsandMaterials))--University of South Australia, 2005.