In-situ geotechnical testing of surficial sediment layers in areas of active sediment dynamics can provide essential information about physical and geotechnical variations of sediment properties with regards to active sediment remobilization processes. For example, portable free fall penetrometers (PFFPs) can assist with the detection of mobile sediment layers. They are easy to deploy, and can provide a large spatial coverage in a time- and cost-effective manner. However, they often struggle to provide more detailed information about the properties of mobile sediment layers due to a lack of calibration and validation in existing data sets. Currently, existing sediment samplers often disturb, or ignore the uppermost sediment layers. Simultaneous sediment sampling and geotechnical profiling is needed to fill this gap, and to drive data interpretation forward. A field investigation of surficial sediments was conducted in the wetland waterways of coastal Louisiana in 2014. In-situ tests were conducted using PFFP, and disturbed sediment samples were collected in selected locations. The results allowed us to map changes in sediment strength and stratification, and correlate the geotechnical results to local site characteristics. However, the need for high quality sediment samples for calibration and validation was emphasized by the results. Three different sediment sampler add-on units targeting mobile layers were designed and manufactured based on lessons-learned from the literature. The designs were tested in the laboratory and in the field (Yakutat, Alaska and York River, Virginia) in 2017. The samples were analyzed to understand the influence of different sampler characteristics on collected sample quality, and, to define mobile layer sampler characteristics that enable simultaneous geotechnical testing and the collection of high quality samples. Following field survey campaigns in the York River, Virginia in 2016 allowed to assess surficial sediment layer characteristics and behavior based on a coupled analysis of geotechnical data from in-situ PFFP tests and the sedimentological data collected using box cores and the novel sediment sampler. In summary, novel strategies and instrumentation to carry out simultaneous sediment sampling and geotechnical profiling of seabed surface layers were tested, and new pathways for geotechnical data analysis for the investigation of mobile seabed layers were presented. / PHD