Neotyphodium are fungal endosymbionts of grasses that reproduce asexually by infecting the hosts seed. This relationship has traditionally been considered mutualistic, with the fungus improving host fitness by alleviating important stresses. To determine the importance of biotic and abiotic stresses in mediating the endophyte-grass interaction, I investigated the relationship between grazing pressure by collared pikas and Neotyphodium sp. infection frequency in the grass Festuca altaica in an alpine meadow. I conducted a factorial design experiment combining endophyte infection, grazing history, fungicide and fertilizer. Leaf demography and herbivory damage were monitored every two weeks. In areas with chronic grazing history, infected plants were significantly less productive than uninfected tussocks, but there was no difference at low grazing history. There was no effect of infection on the likelihood of herbivory. Contrary to predictions of the mutualism theory, the Neotyphodium sp. / F. altaica symbiotum varied from parasitic to neutral across our gradient of interest. / Ecology

Quorum sensing is a cell density-dependent bacterial gene regulatory mechanism used for the expression of colonization-related genes. The symbiotic relationship between the luminescent bacterium Vibrio fischeri and the Hawaiian bobtail squid Euprymna scolopes serves as a model system to study the molecular processes underlying bacterial colonization. This system is especially well-suited for the investigation of the impact of quorum sensing on colonization because (i) it is an easily accessible, natural, two-species colonization model, and (ii) quorum sensing regulates luminescence expression in V. fischeri, which allows the non-invasive detection of quorum-sensing activity both in culture and in symbiosis. While the impact of one of V. fischeri's quorum-sensing systems, lux, on luminescence expression and symbiotic competence has been extensively studied, little was known about other putative systems. The results of this study demonstrate that the V. fischeri ain system is essential for both maximal luminescence expression and symbiotic competence. The ain system predominantly induces luminescence expression at intermediate cell densities, which occur in culture, while the lux system is responsible for luminescence expression at the high cell densities found in symbiosis, suggesting the sequential induction of luminescence gene expression by these two systems. Furthermore, the ain quorum sensing system is important for the processes underlying colonization initiation, while the impact of the lux system is apparent only in later stages of the symbiosis, indicating distinct functions of these two systems during the colonization process. A global transcriptome. analysis of quorum-sensing mutants revealed that ain quorum sensing represses motility gene expression, providing a likely explanation for the initiation defect. Although it has been known that many bacterial species possess multiple quorum-sensing systems, this is the first study demonstrating that two quorum-sensing systems are employed to specifically regulate functions important at distinct cell densities occurring during the colonization process.

Bibliography: leaves 153-185. / [ix], 200, [29] leaves, [6] leaves of plates : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Vesicular-arbuscular (VA) mycorrhiza formation is a complex process which is under the genetic control of both plant and fungus. This project aims to develop a model infection system in Hordeum vulgare L. (barley) suitable for molecular analysis; to identify host plant genes differentially expressed during the early stages of the infection process; and to screen a mutant barley population for phenotypes which form abnormal mycorrhizas. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1995

The overall objective of the work presented in this thesis was to investigate roles of arbuscular mycorrhizal ( AM ) fungi in growth and phosphorus ( P ) nutrition of wheat ( Triticum aestivum L. ) in a highly calcareous soil from the Eyre Peninsula, South Australia. The soil used for this study is one of the main soil types used for wheat production in South Australia. It is severely P - deficient, but plant responses to conventional fertiliser application are poor. Although the total P and Colwell - extractable P contents of the soil are high, the resin - extractable P content is very low. Resin - extractable P is better able to predict P availability for plant growth than Colwell - extractable P. The soil is also strongly P - fixing. Moderate levels ( about 20 mg kg [superscript minus 1] ) of resin - extractable P for wheat could only be achieved by adding high rates ( up to 100 mg kg [superscript minus 1] ) of CaHPO4 in this soil. A bioassay with wheat showed that it can be highly colonised by AM fungi in the soil. AM fungi have been shown to improve P nutrition of plants, particularly in nutrient poor soils. They may thus be important for wheat grown in the soil with low amounts of plant - available P such as the one used. The first part of the work involved conventional pot experiments. Effects of AM fungi on wheat were compared between sterilised soil and non - sterile soil, sterilised soil inoculated with non - sterile soil or with Glomus intraradices or noninoculated, with different soil / sand mixes. Colonisation of wheat at 8 weeks was high, with about 75 % of root length colonised for indigenous fungi and 55 % for Glomus intraradices, regardless of the soil treatments. Growth and P uptake of wheat were significantly increased by both indigenous fungi and G. intraradices, irrespective of soil / sand mixes. Effects of indigenous fungi on plant growth were larger in sterilised and inoculated soil than in non - sterile soil. In sterilised soil, increases of plant growth by AM fungi were higher with G. intraradices than with indigenous fungi. Dilution of the soil by mixing with sand reduced plant growth and P uptake of both AM and non-mycorrhizal ( NM ) plants. In another experiment, responses of wheat to AM fungi and P supply were compared with those of clover. Plants were inoculated with four different AM fungi. Colonisation of wheat was lower than clover. Although suffering from P deficiency, NM wheat ( 6 weeks ) grew relatively well with no added P ( P0 ) and application of P at 100 mg kg [superscript minus 1] ( P100 ) increased the dry weight ( DW ). Shoot P concentrations increased with P application and there were positive effects of all AM fungi at P100. In contrast, NM clover ( 8 weeks ) grew very poorly at P0 and did not respond to P application. Clover responded positively to all AM fungi at both P levels, associated with increases in P uptake. The results showed that responses of wheat to AM inoculation and P supply were quite different from those of clover, and emphasized the different abilities of the two species to access P in the very high P - fixing soil used. Responses of two wheat cultivars ( Brookton and Krichauff ) to AM fungus ( G. intraradices ) were also evaluated with different P supplies at two developmental stages ( vegetative and maturity ). Colonisation by G. intraradices of both cultivars was well established at 6 weeks ( ~ 50 % in P0 plants ) and continued to increase up to maturity ( ~ 70 % ), but decreased greatly at both harvests as P supply was increased ( up to 150 mg P kg [superscript minus 1] ). Addition of P significantly increased plant growth, grain yield and P uptake irrespective of cultivar and harvest time, and the optimum soil P for grain yield was 100 mg kg [superscript minus 1]. In both cultivars, a growth depression in AM plants occurred at 6 weeks at all P levels, but this disappeared at 19 weeks with added P. At P0, AM plants produced lower grain yield per plant, but with higher P supply, AM plants produced higher grain yields than NM plants. There was a significant positive effect of AM on grain P concentration at P0, but not at other P levels. Brookton was somewhat more P efficient than Krichauff, and the latter responded more to AM fungi. The results showed that responses of wheat to AM fungi and P supply changed during development. Growth depression induced by AM fungi in low P soil was overcome by addition of moderate amounts of P, resulting in significant increases in grain yield in AM plants. Additional approaches were used to help determine the roles of AM fungi in wheat growth and nutrition. The effects of plant density were tested, as it was expected that increasing density might decrease the negative effects of AM fungi on wheat growth. Large growth depressions were induced by both G. intraradices and Gigaspora margarita in wheat grown at low density, although % colonisation by G. intraradices was higher than by Gi. margarita. With increasing plant density, the growth depressions were smaller, indicating that competition modulates growth responses. Although there may be effects due to competition for soil P, it is clear that with increasing plant biomass per unit soil volume, the AM fungal biomass did not increase in proportion ; in fact, hyphal length density decreased. Accordingly, costs of AM in terms of organic carbon loss per plant decreased with increasing plant density, thus mitigating the growth depression. The results add to the increasing body of evidence that mycorrhizal growth responses of plants grown singly may not apply at the population or community level as in crops. Two compartmented pot systems were used to examine whether the fungal hyphae deliver the P into the plants even in the absence of positive growth responses. An experiment in which plants were constricted in a mesh bag, but hyphae of AM fungi could explore a large soil volume was carried out. Results suggested that AM fungi helped the plants acquire P, although mesh bags did not remove AM growth depression. The experiments in which AM fungi were supplied with [superscript 32]P in a small soil compartment to which only hyphae had access showed that a considerable amount of P was delivered to wheat plants via AM fungi. The original aim was to calculate the percentage of total P entering the plants via the AM pathway. However, realistic values were not obtained probably because of difficulties of determining plant-available P and uneven distribution of hyphae in the soil. It is also possible that plants and AM fungi access different P pools. This study demonstrated the potential roles of AM fungi in growth and P nutrition of wheat grown in the highly calcareous soil from the Eyre Peninsula, South Australia. Further studies on the effects of the interactions between AM fungi and wheat in the field are needed to assess the contribution of AM fungi to plant nutrition. / Thesis (Ph.D.)--School of Earth and Environmental Sciences, 2005.

[Truncated abstract] Some plants, such as legumes, are able to use atmospheric nitrogen as a nitrogen source due to the nitrogen-fixing bacteria residing in specialised root structures called nodules. The exchange of carbon and nitrogen between the host plant (legume) and the nitrogen- fixing micro-symbiont is vital for biological nitrogen fixation. In particular, transport of C4-dicarboxylates, mainly malate, from the plant to the micro-symbiont, and the reverse transport of fixed nitrogen in the form of ammonium are essential for symbiotic nitrogen fixation. In the legume nodule, the symbiosome membrane (SM) surrounds the bacteroid and all exchanges of metabolites and nutrients that occur between the plant and the micro-symbiont must cross this membrane. Recently it has been established that cycling of amino acids across the SM is also critical for optimal symbiotic nitrogen fixation. Therefore to fully understand this agriculturally significant phenomenon, the mechanisms facilitating these exchanges need to be investigated. The major aim of this study was to increase the understanding of nutrient exchange within the nodule at the molecular level by isolating and characterising genes encoding transporters responsible for malate and amino acids transport in soybean (Glycine max, L.), with particular interest in genes significantly or highly expressed in nodules. A combination of molecular and biochemical techniques was used to achieve this. ... Southern blot analysis showed that a small gene family of up to five members encodes these proteins in soybean. A full-length cDNA, designated GmAAP5, was isolated that encodes a novel, putative amino acid transporter. Molecular characterisation of this cDNA and that of GmAAP1 (GenBank Accession no: AY029352), a previously identified putative amino acid transporter gene, was done. Expression analyses showed relatively high expression of GmAAP5 in soybean nodules compared to that in leaf and root tissues, while GmAAP1 showed uniformly high expression in root, leaf and nodule tissues. Phylogenetic analysis of the deduced amino acid sequences of known functional AAPs from dicotyledonous plants revealed that GmAAP1 is most closely related to AAP2 from V. faba, while GmAAP5 is more closely related to AAPs from non-leguminous plants than from leguminous plants. Based on the functional characterisation of the AAPs with which GmAAP1 and GmAAP5 cluster, it is likely that both transporters are neutral and acidic amino acid transporters within the AAP subfamily.

Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Science, 1996? / Includes bibliographical references (leaves 153-185).

Thesis (M.Sc.)--Victoria University of Wellington, 2008. / Includes bibliographical references.

Thesis (M.Arch.)--University of Detroit Mercy, 2008. / "April 28, 2008". Includes bibliographical references (p. [19-21]).

Thesis (M.E.S.)--Evergreen State College, 2008. / "December, 2008." Title from title screen (viewed 4/8/2010). Includes bibliographical references (leaves 22-28).

Thesis (Ph. D.)--University of Texas at Austin, 2005. / Vita. Includes bibliographical references.