Ergot alkaloids present in endophyte-infected (E+) tall fescue are thought to be the causative agent of fescue toxicosis, a syndrome affecting cattle in the eastern United States. Many of the observed signs of fescue toxicosis are thought to be attributed to peripheral vasoconstriction; however, there are data indicating that ergot alkaloids can alter blood flow to the gut. An experiment was conducted using right ruminal artery and vein collected from heifers shortly after slaughter. Vessels were mounted in a multi-myograph to determine the vasoconstrictive potentials of ergot alkaloids present in E+ tall fescue. Results indicated ergot alkaloids have the potential to induce vasoconstriction of foregut vasculature. A second experiment was conducted to determine if the additional ergot alkaloids present in E+ tall fescue increase the vasoconstrictive response above that of ergovaline. Results indicated that ergovaline is the main alkaloid responsible for vasoconstriction in bovine vessels. A third study was performed to determine the effect of ergot alkaloids on ruminal epithelial blood flow in the washed rumen of steers exposed to E+ or endophyte-free (E-) tall fescue seed. Steers were dosed with seed followed by a washed rumen experiment with differing levels of ergovaline incubated in the rumen. Results indicated that E+ tall fescue seed treatment reduced ruminal epithelial blood flow. Additionally, incubating ergovaline in the rumen during the washed rumen further decreased epithelial blood flow and volatile fatty acid (VFA) absorption. A final study was conducted to determine the acute effects of ergot alkaloids on isolated rumen epithelial absorptive and barrier functions and the potential for ruminal ergovaline absorption. Results indicate that acute exposure to ergot alkaloids does not alter the absorptive or barrier function of rumen epithelium and ergovaline is absorbed from the rumen. Data from this series of experiments have shown that ergot alkaloids from E+ tall fescue can induce vasoconstriction of blood vessels in the foregut of cattle, reduce blood flow to the rumen epithelium, and decrease VFA absorption. The decrease in nutrient absorption could contribute to the observed symptoms of fescue toxicosis, including depressed growth rates and general unthriftiness.

Two experiments were conducted at the University of Kentucky, Eden Shale Farm, Owenton, KY to evaluate the use of MTB-100TM (Alltech, Inc., Nicholasville, KY) to alleviate the symptoms of fescue toxicity when lactating Angus x Beefmaster cows and their calves grazed endophyte-infected KY-31 tall fescue. Experiment 1 provided a carbohydrate based toxin adsorbent, MTB-100TM, ad libitum in a commercial mineral supplement to project a daily consumption rate of 0, 20 or 40 g of MTB-100TM per cow. Cows were weighed, assigned a body condition score (BCS) and hair coat score (HC), rectal temperatures were recorded and fecal grab samples were taken for ergovaline (EV) and lysergic acid (LA) analysis every 35 days for three grazing seasons (May to September). Calves were also weighed and assigned a HC score. Although MTB-100TM did not improve cow or calf performance, cows older than 4 years and those with greater Beefmaster breeding exhibited a higher tolerance to fescue toxicity than 2 and 3-yr-olds and cows with greater Angus breeding. Experiment 2 was conducted to evaluate the response of lactating beef cows and their calves to strategic supplementation with MTB-100TM. MTB-100TM was mixed with a complete mineral so daily intake was projected to be 0 or 20 g/cow. The experimental period extended from May 5 to October 2 and was divided into 3 strategic periods: P1 = May 5 to July 5; P2 = July 5 to August 31; P3 = August 31 to October 2. Treatments were either 0 or 20 g•cow-1•d-1 MTB-100TM within a period (Treatment 1 = 0, 0, 0; Treatment 2 = 20, 0, 20; Treatment 3 = 0, 20, 0; Treatment 4 = 20, 20, 0; and Treatment 5 = 20, 20, 20). Cow and calf performance was measured the same as Exp. 1, but every 21 days. Supplementation early in the grazing season tended to improve cow weight gain and body condition; however, there was no effect on calf performance. Fecal output of EV and LA did not increase in either experiment with supplementation. In conclusion, strategically invoked MTB-100TM consumption can increase performance of cows grazing endophyte-infected tall fescue forage.

Photocopy of typescript. / Digitized by Kansas Correctional Industries

The use of nitrogen (N) fertilizer is generally restricted on mixed species forage systems due to its stimulatory effect on grasses which increases competition with legume species. Reduced legume growth from this competition can compromise forage nutritive value and prospective yields. The controlled-release nature of several enhanced efficiency fertilizer N products holds the potential to improve legume persistence in mixed species pastures while providing supplemental N required by the grass component. The studies contained in this dissertation evaluated the effect of different enhanced efficiency N formulations (ATU, ESN, methylene urea, SuperU, and a 75% ESN: 25% urea blend) and untreated urea on yield, nutritive value, and legume persistence in a ‘Wrangler’ bermudagrass and ‘Durana’ white clover mixture (2014-2016 growing seasons), ‘KY-31’tall fescue and ‘Kenland’ red clover mixture (2015-2016 growing seasons), and ‘KY-31’ stockpiled tall fescue (2015-2017). The three studies were conducted at the University of Kentucky Spindletop Research Farm in Lexington, KY in a randomized complete block design. In the bermudagrass-white clover study, all enhanced efficiency N sources maintained white clover populations similar to the unfertilized grass/clover control, but only ESN caused greater clover composition than standard urea. Total forage yields increased linearly with N rate in all years, but dry weather conditions in the second and third years resulted in lower total yield. Forage nutritive value followed general trends throughout each growing season, but ESN’s ability to maintain clover resulted in higher nutritive value. In the tall fescue-red clover, total forage yields curvilinearly increased with N rate in 2015 but did not vary in 2016. ESN and ESN+urea blend treatments retained clover composition similar to that of the unfertilized control. Stockpiled forage yield increased with higher N rates. Enhanced efficiency N fertilizers with the ability to control N release can enhance forage yield while maintaining clover in mixed species swards.

Cool season perennial grasses are the foundation of equine nutrition in the transition zone. The objective of this study was to evaluate forage quality using ADF, NDF, IVTDMD, CP, WSC, and ESC and changes in vegetative swards seasonally, diurnally, across species (Kentucky bluegrass, tall fescue, orchardgrass, and perennial ryegrass) and cultivar. This study was conducted in 2015 and 2017 and plots were maintained vegetatively with two to four week mowing. Morning and afternoon sample collection occurred monthly during the growing season. Samples were flash frozen; freeze dried, ground, and scanned using Near Infrared Reflectance Spectroscopy (NIRS) to predict forage quality. There was a significant year effect; therefore year was analyzed separately. Generally, ADF and NDF were highest for Kentucky bluegrass (30 and 52%), lowest for perennial ryegrass (25 and 46%), and tall fescue and orchardgrass were inconsistent. Crude protein was variable across species and season, ranging 10 to 25%. ADF and NDF concentrations were higher in the morning; IVTDMD, WSC, and ESC were higher in the afternoon; and CP was similar diurnally. In conclusion, forage quality in vegetative cool season grass pastures was sufficient to meet the nutritional needs of most equines, but varied seasonally, diurnally, across species, and cultivar.

Graduation date: 1998

Endophytic fungi (A. coenophialum and A. lolii) which infect grasses produce ergot alkaloids that serve as the grasses' chemical defenses and enhance the vigor of the grass. Turf-type tall fescue with high endophyte levels has been deliberately developed to produce a greener, more vigorous, pest-resistant turf. Consumption of endophyte-infected grass causes various toxicity symptoms in livestock. Cattle in the southeastern and midwestern United States, where tall fescue is grown on 14 million hectares, often develop signs of toxicosis during summer months from grazing plants in fected by A. coenophialum. A more severe form of the disease, fescue foot, has been associated with cold environment and reported in late fall and winter months not only in the southeastern United States but also in the northwest United States. In New Zealand, where perennial ryegrass is grown on 7 million hectares of pasture, sheep often develop a condition called ryegrass staggers from grazing plants infected by A. lolii. New Zealand reports economic losses grazing plants infected by A. lolii. New Zealand reports economic losses associated with the sheep industry of $205 million per year. In the United States, economic losses associated with the beef cattle industry alone is estimated at $600 million per year. Range finding experiments and case studies of fescue foot and perennial ryegrass staggers (PRGS) were conducted on cattle and sheep under grazing and barn conditions. The main objective was to determine threshold levels of the endophyte toxins, ergovaline (EV) (appendix 1) and lolitrem B (appendix 2), associated with the diseases of fescue foot and PRGS respectively. Fescue foot was experimentally induced in cattle under barn studies in the spring with 825 ppb ergovaline. The ergovaline contaminated feed was given for a period of 42 days. Similar barn studies in sheep in spring to early summer did not produce clinical fescue foot with up to 1215 ppb. Field studies of natural fescue foot in a herd of sheep were conducted, (ie 540 ppb) values of ergovaline in the feed, but clinical disease was not produced in late fall through winter. A case study from a herd of sheep revealed 813 ppb dietary ergovaline had produced fescue foot in the months of fall (November). Fields of perennial ryegrass (PRG) where sheep received 2,135 ppb lolitrem B toxin were associated with clinical cases of PRGS in 42 sheep of 237 sheep (18 percent incidence rate) in the Willamette Valley of Oregon. Three months later, sheep on this same field which then had 1,465 ppb lolitrem B, did not have PRGS. These were the first range finding experiments undertaken in this locale to document threshold levels of endophyte toxins associated with fescue foot and PRGS. / Graduation date: 1994

A series of experiments were conducted during 2005 and 2006 to evaluate five perennial grass species for forage yield and quality, steer performance and grazing capacity, animal intake, plant energy reserves and economic return under grazed conditions. In 1999, two 0.8 ha replicates each of Paddock meadow bromegrass (<i>Bromus riparius</i>Rehm.), Carlton smooth bromegrass (<i>Bromus inermis</i>Leyss.) and AC Knowles hybrid bromegrass (<i>B. riparius</i> x <i>B. inermis</i>) were seeded. In 2003, two 0.8 ha replicates each of AC Goliath crested wheatgrass (<i>Agropyron cristatum </i> (L.) Gaertn.), hybrid bromegrass, and Courtenay tall fescue (<i>Festuca arundinacea </i> Schreb.) were seeded. A long established stand of crested wheatgrass acted as the control pasture. For 2003 established pastures, AC Goliath crested wheatgrass (7515 kg ha-1) had greater (P<0.05) cumulative dry matter yield than hybrid bromegrass (3136 kg ha-1) during the 2005 grazing season. Average (2005-2006) crude protein (CP) was greatest (P<0.05) for hybrid and smooth bromegrass for 1999 established pastures at start and middle of period one. Control pastures had the greatest (P<0.05) neutral detergent fiber (NDF) mid-grazing period. Over 2 years, smooth bromegrass had greater acid detergent fiber (ADF) (P<0.05) than control pastures at the end of the grazing period one. Average (2005-2006) in vitro organic matter digestibility (IVOMD) was greatest for hybrid and meadow bromegrass (P<0.05) at the start of grazing period one. Control pastures (129 g kg-1) had lower CP levels at the start of the 2005-2006 (average) grazing period 1 (P<0.05) compared to species seeded in 2003. Control and hybrid bromegrass pastures had the greatest NDF and ADF levels at the start of grazing period 1 (2005-2006 average) while tall fescue pastures had the lowest (P<0.05) NDF and ADF levels. Over 2 years, control pastures had the lowest IVOMD at start of grazing (P<0.05). In 2006, hybrid and smooth bromegrass had greater etiolated re-growth than control pastures (P<0.05). In 2006, grazed plants seeded in 1999 had greater (P<0.05) etiolated re-growth than ungrazed plants. For 2003 seeded grasses, crested wheatgrass produced greater (P<0.05) etiolated re-growth than tall fescue and control pastures. Average daily gain was similar (P>0.05) for all 1999 and 2003 seeded grasses. Overall, bromegrasses seeded in 1999 produced greater animal grazing days (AGD) than control pastures (P<0.05). Total beef production (TBP) was greater (P<0.05) for hybrid and meadow bromegrass compared to the control. All species seeded in 2003 produced greater AGD (P<0.05) compared to the control. Crested wheatgrass produced greater (P<0.05) TBP than the control over both years of the study. The C33:C32 alkane ratio estimated greater DMI (P<0.05) for hybrid bromegrass (9.9 kg d-1) and control pastures (9.6 kg d-1) compared to crested wheatgrass (6.8 kg d-1) or tall fescue (6.8 kg d-1) during period 1 in 2006. Over 2 years, net return to labor, equity and personal draw was greater (P<0.05) for hybrid bromegrass ($91.24 ha-1) compared to the control (-$54.32 ha-1). For 2003 seeded pastures, all pastures generated positive returns over 2 years. Crested wheatgrass ($92.49 ha-1) had greater net return than control pastures (-$54.32 ha-1) (P<0.05). Finally, the results of this grazing study indicate beef producers can manage these grasses during the summer grazing season and maintain high levels of animal performance and pasture production. This study has demonstrated that bromegrasses, crested wheatgrass and tall fescue could work well in a complementary grazing system.

A series of experiments were conducted during 2005 and 2006 to evaluate five perennial grass species for forage yield and quality, steer performance and grazing capacity, animal intake, plant energy reserves and economic return under grazed conditions. In 1999, two 0.8 ha replicates each of Paddock meadow bromegrass (<i>Bromus riparius</i>Rehm.), Carlton smooth bromegrass (<i>Bromus inermis</i>Leyss.) and AC Knowles hybrid bromegrass (<i>B. riparius</i> x <i>B. inermis</i>) were seeded. In 2003, two 0.8 ha replicates each of AC Goliath crested wheatgrass (<i>Agropyron cristatum </i> (L.) Gaertn.), hybrid bromegrass, and Courtenay tall fescue (<i>Festuca arundinacea </i> Schreb.) were seeded. A long established stand of crested wheatgrass acted as the control pasture. For 2003 established pastures, AC Goliath crested wheatgrass (7515 kg ha-1) had greater (P<0.05) cumulative dry matter yield than hybrid bromegrass (3136 kg ha-1) during the 2005 grazing season. Average (2005-2006) crude protein (CP) was greatest (P<0.05) for hybrid and smooth bromegrass for 1999 established pastures at start and middle of period one. Control pastures had the greatest (P<0.05) neutral detergent fiber (NDF) mid-grazing period. Over 2 years, smooth bromegrass had greater acid detergent fiber (ADF) (P<0.05) than control pastures at the end of the grazing period one. Average (2005-2006) in vitro organic matter digestibility (IVOMD) was greatest for hybrid and meadow bromegrass (P<0.05) at the start of grazing period one. Control pastures (129 g kg-1) had lower CP levels at the start of the 2005-2006 (average) grazing period 1 (P<0.05) compared to species seeded in 2003. Control and hybrid bromegrass pastures had the greatest NDF and ADF levels at the start of grazing period 1 (2005-2006 average) while tall fescue pastures had the lowest (P<0.05) NDF and ADF levels. Over 2 years, control pastures had the lowest IVOMD at start of grazing (P<0.05). In 2006, hybrid and smooth bromegrass had greater etiolated re-growth than control pastures (P<0.05). In 2006, grazed plants seeded in 1999 had greater (P<0.05) etiolated re-growth than ungrazed plants. For 2003 seeded grasses, crested wheatgrass produced greater (P<0.05) etiolated re-growth than tall fescue and control pastures. Average daily gain was similar (P>0.05) for all 1999 and 2003 seeded grasses. Overall, bromegrasses seeded in 1999 produced greater animal grazing days (AGD) than control pastures (P<0.05). Total beef production (TBP) was greater (P<0.05) for hybrid and meadow bromegrass compared to the control. All species seeded in 2003 produced greater AGD (P<0.05) compared to the control. Crested wheatgrass produced greater (P<0.05) TBP than the control over both years of the study. The C33:C32 alkane ratio estimated greater DMI (P<0.05) for hybrid bromegrass (9.9 kg d-1) and control pastures (9.6 kg d-1) compared to crested wheatgrass (6.8 kg d-1) or tall fescue (6.8 kg d-1) during period 1 in 2006. Over 2 years, net return to labor, equity and personal draw was greater (P<0.05) for hybrid bromegrass ($91.24 ha-1) compared to the control (-$54.32 ha-1). For 2003 seeded pastures, all pastures generated positive returns over 2 years. Crested wheatgrass ($92.49 ha-1) had greater net return than control pastures (-$54.32 ha-1) (P<0.05). Finally, the results of this grazing study indicate beef producers can manage these grasses during the summer grazing season and maintain high levels of animal performance and pasture production. This study has demonstrated that bromegrasses, crested wheatgrass and tall fescue could work well in a complementary grazing system.

Aircraft strikes are a significant safety hazard on airports worldwide. Wildlife management at airfields is the most effective tactic to reduce airstrike risk – to modify the habitat to be undesirable to animals. Tall fescue grasses containing a fungal symbiont may serve that purpose. They produce alkaloids that convey resistance to some grass-feeding invertebrates, which might in turn reduce incidence of insectivorous birds. A commercial endophytic grass (Avanex™) consisting of ‘Jackal’ tall fescue infected with a unique endophyte (AR 601) is purported to contain especially high levels of alkaloids and to reduce bird populations if planted at airports. I evaluated it against the common KY31 tall fescue with its wild-type endophyte for invertebrate and vertebrate deterrence. Invertebrate abundance, survival, growth, and development were generally similar on Jackal E+ or KY31 E+. Spanish goats and wild birds showed no avoidance of Jackal E+, nor did Jackal E+ contain significantly higher levels of alkaloids than did KY31 E+. The Avanex™ tall fescue was not any better than KY31 in deterring herbivores but the concept is sound. However, better understanding of the relationship between grass, endophyte, alkaloid, and herbivore is needed to inform how such grasses might be used to reduce bird strike hazard.