Yang, Kar Lai Alice. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts also in Chinese.

by Tang Shuk Chun. / "September 1998." / Thesis (Ph.D.)--Chinese University of Hong kong, 1998. / Includes bibliographical references (p. 157-178). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Bacteria are inarguably the most ubiquitous and adaptive organisms on the planet. The vast, diverse community of microbes residing in soil are mostly studied using sequencing technologies because over 99% of them are currently uncultivable in the laboratory. This lack of diverse bacterial cultivation presents a serious challenge for modern microbiological and medical science where the discovery of novel antibiotic producers and microbial products has been outpaced by the rise in drug resistance. This study designed and tested two new cost-effective culture systems called the “Dynamic Soil Environment” and Soil Extract Systems with the goal of increasing the cultivable communities of diverse bacteria in a soil sample over standard methods. Illumina MiSeq sequencing and DADA2 pipeline protocols were used to analyze community DNA from cultivated samples and source soil metagenomes. Autoclaved soil extract media in the Soil Extract Experiment yielded a statistically significantly greater Shannon’s (p = 0.008) and Simpson’s diversity (p = 0.007) of bacteria over pH modified (6.4) nutrient agar media over 30 days of incubation. Autoclaved soil extract media was also able to cultivate, on average, 33% of species in bulk soil sequences compared to 27% from standard nutrient agar however these differences weren’t statistically significant. The length of incubation had a lesser effect than media type on yield of bacteria over 30 days in batch culture conditions. Species richness and diversity generally decreased over time except in soil extract samples. In the Dynamic Soil Environment experiment, membrane plates placed on a live soil environment produced a slightly higher diversity than autoclaved membrane plates and control plates without soil, however, these differences were not statistically significant except when analyzed with Chao1 diversity (0.041). Cultivated bacterial diversity and communities differed more according to media type than soil environment with statistically significant differences between standard and pH modified nutrient agar. Media with a 5.8 pH buffer produced a significantly higher relative abundance of the well-known antibiotic-producers, Actinobacteria (t(10) = -5.715, p < .000) and also Proteobacteria (t(10) = -10.127, p < .000). This study establishes cost-effective methods of cultivating more diverse bacterial communities for low-funded laboratories. Culture conditions for the reliable cultivation of higher relative abundances of bacterial groups belonging to Actinobacteria and Proteobacteria are also established with the Dynamic Soil Environment Experiment. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2019. / FAU Electronic Theses and Dissertations Collection

Indiana University-Purdue University Indianapolis (IUPUI) / Anchorage control is fundamental to successful orthodontic treatment. Dental implants can serve as ideal anchorage units because of their stability in bone. Previous studies limit the use of existing implants for anchorage because of their large size. Minimizing the size of the implant would reduce the extent of the surgery and may result in a decreased and less traumatic healing period. The objective of this study was to histomorphometrically analyze the use of miniature implants. A total of 96 miniature implants (1.0 x 5.0 mm; 48 loaded and 48 healing control) were placed in the mandible and maxilla of 8 male dogs. The implants were allowed to heal for three different periods (3, 6, and 12 weeks) followed by 12 weeks of 200 to 300 g of orthodontic force application. Bone specimens containing implants were collected for histomorphometric analysis. The results indicate that clinical rigidity (osseointegration) was achieved by 96.9 percent of the miniature implants. Histomorphometric analysis revealed that the amount of bone contact at the implant-bone interface ranged from 11.3 to 68 percent (mean ± SEM=34.4 ± 4.6 percent) in the healing control groups and from 18.8 to 63 percent (mean=43.l ± 4.0 percent) in the force applied groups in the maxilla. On the other hand, in the mandible, bone-implant contact ranged from 7 to 82 percent (mean=44.1 ± 6.8 percent) in the healing control groups and from 12 to 72 percent (mean=50.7 ± 5.3 percent) in the force applied groups. Results from bone formation rate, mineralizing surface/bone surface and mineral appositional rate showed a significant difference in the 3-week healing control group compared to those in other groups. From these results, we concluded that miniature implants are able to function as rigid osseous anchorage for orthodontics with minimal (less than 3 weeks) healing period. This study was supported by Matsumoto Research fund.

Fong Shu Pan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 147-167). / Abstracts in English and Chinese. / ACKNOWLEDGEMENTS --- p.i / LIST OF PUBLICATIONS --- p.ii / ABSTRACT --- p.iii / ABSTRACT [IN CHINESE] --- p.vii / TABLE OF CONTENT --- p.ix / LISTS OF FIGURES --- p.xv / LIST OF TABLES --- p.xxi / LIST OF ABBREVATIONS --- p.xxii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Embryonic stem (ES) cells --- p.1 / Chapter 1.2 --- Stem cell plasticity --- p.5 / Chapter 1.2.1 --- Differentiation and trans-differentiation of lineage-restricted stem cells --- p.5 / Chapter 1.2.1.1 --- Multilineage differentiation in-vitro --- p.5 / Chapter 1.2.1.2 --- Trans-differentiation --- p.6 / Chapter 1.2.2 --- Prospective applications of stem cells --- p.7 / Chapter 1.2.2.1 --- Basic research on development --- p.7 / Chapter 1.2.2.2 --- Study of human disease --- p.7 / Chapter 1.2.2.3 --- Cancer research --- p.7 / Chapter 1.2.2.4 --- Drug screening --- p.8 / Chapter 1.2.2.5 --- Cell therapy --- p.8 / Chapter 1.3 --- Neuro-degenerative diseases and cell therapy --- p.9 / Chapter 1.3.1 --- Neuro-degenerative diseases --- p.9 / Chapter 1.3.2 --- Neuro-regeneration --- p.10 / Chapter 1.3.3 --- Cell sources for neuro-regenerative therapy --- p.11 / Chapter 1.3.3.1 --- Comparison of stem cells --- p.11 / Chapter 1.3.3.2 --- Stem cells in neuro-regenerative therapy --- p.12 / Chapter 1.4 --- In-vitro derivation into neural lineage --- p.17 / Chapter 1.4.1 --- In-vitro induction strategies available --- p.17 / Chapter 1.4.1.1 --- Chemical agents --- p.18 / Chapter 1.4.1.1.1 --- Retinoic acid (RA) --- p.18 / Chapter 1.4.1.1.2 --- Ascorbic acid --- p.19 / Chapter 1.4.1.2 --- Growth factors/cytokines --- p.19 / Chapter 1.4.1.2.1 --- Neurotrophins --- p.20 / Chapter 1.4.1.2.2 --- Stimulants --- p.20 / Chapter 1.4.1.2.3 --- Signalling molecules --- p.21 / Chapter 1.4.1.3 --- Culture Selection --- p.23 / Chapter 1.4.1.3.1 --- Conditions --- p.23 / Chapter 1.4.1.3.2 --- Medium --- p.23 / Chapter 1.4.1.4 --- Transfection of regulator genes using viral vector --- p.24 / Chapter 1.4.1.5 --- Stromal cell-derived inducing activity (SDIA) --- p.26 / Chapter Chapter 2 --- Aims --- p.28 / Chapter 2.1 --- Hypothesis and study objectives --- p.28 / Chapter 2.1.1 --- Soliciting an optimal method for ES cell propagation --- p.28 / Chapter 2.1.2 --- Pursuing alternative SDIA --- p.29 / Chapter Chapter 3 --- Materials and Methods --- p.33 / Chapter 3.1 --- Chemicals and Reagents --- p.33 / Chapter 3.1.1 --- Cell Culture --- p.33 / Chapter 3.1.2 --- Immunohistochemistry and staining --- p.35 / Chapter 3.1.3 --- Molecular Biology --- p.36 / Chapter 3.2 --- Consumable --- p.37 / Chapter 3.3 --- Cell lines --- p.39 / Chapter 3.3.1 --- Feeder cells --- p.39 / Chapter 3.3.1.1 --- Primary mouse embryonic fibroblasts --- p.39 / Chapter 3.3.1.2 --- STO --- p.39 / Chapter 3.3.1.3 --- L Cells --- p.40 / Chapter 3.3.1.4 --- L-Wnt-3A Cells --- p.40 / Chapter 3.3.1.5 --- C17.2 --- p.40 / Chapter 3.3.2 --- ES cells --- p.41 / Chapter 3.3.2.1 --- ES-D3 --- p.41 / Chapter 3.3.2.2 --- ES-E14TG2a --- p.41 / Chapter 3.4 --- In-house prepared solutions --- p.42 / Chapter 3.4.1 --- "Stock solution of Insulin, Transferrin, Selentine (ITS) Supplement" --- p.42 / Chapter 3.4.2 --- Enriched Knock-Out Dulbecco's Modified Eagle's Medium (KO DMEM) --- p.42 / Chapter 3.4.3 --- Mitomycin C solution --- p.42 / Chapter 3.4.4 --- Gelatin solution 0.1% --- p.42 / Chapter 3.4.5 --- p-mercaptoethanol solution --- p.43 / Chapter 3.4.5.1 --- (3-mercaptoethanol solution 0.1M --- p.43 / Chapter 3.4.5.2 --- P-mercaptoethanol solution 0.1M --- p.43 / Chapter 3.4.5.3 --- p-mercaptoethanol solution 0.1M for preparation of culture medium --- p.43 / Chapter 3.4.6 --- ALL-trans retinoic acid --- p.43 / Chapter 3.4.6.1 --- ALL-trans retinoic acid stock solution 0.01M --- p.43 / Chapter 3.4.6.2 --- ALL-trans retinoic acid working solution lμM --- p.43 / Chapter 3.4.7 --- Paraformaldehyde solution 4% (PFA) --- p.44 / Chapter 3.4.8 --- TritoxX-100 solution --- p.44 / Chapter 3.4.8.1 --- Tritox X-100 solution 3% --- p.44 / Chapter 3.4.8.2 --- Tritox X-100 solution 0.3% --- p.44 / Chapter 3.4.9 --- Popidium iodide solution lug/mL (PI) --- p.44 / Chapter 3.4.10 --- Geneticin solution --- p.45 / Chapter 3.4.10.1 --- Geneticin solution 50mg/mL --- p.45 / Chapter 3.4.10.2 --- Geneticin solution 5mg/mL --- p.45 / Chapter 3.4.11 --- Poly-L-ornithine solution --- p.45 / Chapter 3.4.12 --- Laminin solution --- p.45 / Chapter 3.4.13 --- Maintenance medium for cell feeders --- p.46 / Chapter 3.4.14 --- Mitomycin C inactivation medium --- p.46 / Chapter 3.4.15 --- Freezing medium --- p.46 / Chapter 3.4.16 --- Propagation medium for ES cells --- p.47 / Chapter 3.4.16.1 --- Serum-based propagation medium for ES cells --- p.47 / Chapter 3.4.16.2 --- Serum-free propagation medium for ES cells --- p.47 / Chapter 3.4.16.3 --- Serum-free induction medium for ES cells --- p.48 / Chapter 3.4.16.3.1 --- Serum-free induction medium 1 --- p.48 / Chapter 3.4.16.3.2 --- Serum-free induction medium II --- p.48 / Chapter 3.4.16.3.3 --- Serum-free induction medium III --- p.48 / Chapter 3.5 --- Equipments --- p.49 / Chapter 3.6 --- Methods --- p.50 / Chapter 3.6.1 --- Cell Culture --- p.50 / Chapter 3.6.1.1 --- Preparation of round cover-slips --- p.50 / Chapter 3.6.1.2 --- Gelatinization of tissue culture wares --- p.51 / Chapter 3.6.1.3 --- Poly-L-ornithine and laminin coating --- p.51 / Chapter 3.6.1.4 --- Thawing frozen cells --- p.51 / Chapter 3.6.1.5 --- Passage of adherent culture --- p.52 / Chapter 3.6.1.6 --- Cell count --- p.52 / Chapter 3.6.1.7 --- Cytospin --- p.53 / Chapter 3.6.1.8 --- Cell viability test --- p.53 / Chapter 3.6.1.9 --- Cryopreservation --- p.53 / Chapter 3.6.1.10 --- Preparation of primary mouse embryonic fibroblast (PMEF) --- p.54 / Chapter 3.6.1.11 --- Mitomycin C inactivation of feeder cells --- p.55 / Chapter 3.6.1.12 --- Gamma irradiation of various feeders --- p.55 / Chapter 3.6.1.13 --- Preparation of CM from feeder cells --- p.56 / Chapter 3.6.1.14 --- Propagation of ES cells in serum-based medium --- p.56 / Chapter 3.6.1.15 --- Propagation of ES cell in serum-free medium --- p.56 / Chapter 3.6.1.16 --- Neural differentiation using all-trans retinoic acid --- p.57 / Chapter 3.6.1.17 --- Stromal cells-derived inducing activity --- p.58 / Chapter 3.6.1.18 --- BrdU labeling of the cell products --- p.59 / Chapter 3.6.2 --- Molecular analysis --- p.60 / Chapter 3.6.2.1 --- RNA extraction --- p.60 / Chapter 3.6.2.2 --- RNA quantitation --- p.60 / Chapter 3.6.2.3 --- Reverse Transcription of the First Strand complementary DNA --- p.61 / Chapter 3.6.2.4 --- Polymerase chain reaction --- p.61 / Chapter 3.6.2.5 --- RNA Integrity Check --- p.66 / Chapter 3.6.2.6 --- Electrophoresis and visualization of gene products --- p.66 / Chapter 3.6.3 --- Immunofluoresent staining --- p.66 / Chapter 3.6.4 --- In-vivo studies --- p.69 / Chapter 3.6.4.1 --- Induction of cerebral ischaemia in mice --- p.69 / Chapter 3.6.4.2 --- Transplantation --- p.69 / Chapter 3.6.4.3 --- Assessment of learning ability and memory --- p.70 / Chapter 3.6.5 --- Histological analysis --- p.70 / Chapter 3.6.5.1 --- Animal sacrifice for brain harvest --- p.70 / Chapter 3.6.5.2 --- Cryosectioning --- p.71 / Chapter 3.6.5.3 --- Paraffin sectioning --- p.71 / Chapter 3.6.5.4 --- Haematoxylin and eosin staining --- p.72 / Chapter 3.7 --- Data analysis --- p.73 / Chapter Chapter 4 --- Results --- p.74 / Chapter 4.1 --- ES cell maintenance --- p.74 / Chapter 4.1.1 --- Serum effect --- p.74 / Chapter 4.1.2 --- Feeder effect --- p.79 / Chapter 4.1.3 --- Serum-free and feeder-free condition --- p.86 / Chapter 4.1.4 --- Overall effect --- p.89 / Chapter 4.2 --- ES cell Induction --- p.91 / Chapter 4.2.1 --- Retinoic acid --- p.91 / Chapter 4.2.2 --- Stromal cell-derived inducing activity --- p.96 / Chapter 4.2.2.1 --- Molecular characterization of candidate stromal cells --- p.96 / Chapter 4.2.2.2 --- Direct contact co-culture --- p.98 / Chapter 4.2.2.3 --- Non-contact co-culture --- p.100 / Chapter 4.2.2.4 --- Cultures in CM --- p.109 / Chapter 4.3. --- ES cell Differentiation --- p.115 / Chapter 4.4 --- In vivo study of ES cell-derived cell products --- p.117 / Chapter 4.4.1 --- Animal preparation --- p.117 / Chapter 4.4.2 --- Cell preparation --- p.117 / Chapter 4.4.3 --- Cell implantation --- p.117 / Chapter 4.4.4 --- Behaviour Monitoring --- p.121 / Chapter 4.4.5 --- Histology of cell-implanted brain --- p.125 / Chapter Chapter 5 --- Discussion --- p.129 / Chapter Chapter 6 --- Conclusion --- p.144 / References --- p.147

Indiana University-Purdue University Indianapolis (IUPUI) / The current movement in dentistry is to provide treatment that is evidence-based rather than opinion-based. Unfortunately, there is a lack of evidence for most orthodontic appliances. Much work has been done to find the appropriate load system to move teeth, but research has only been done with laboratory techniques that may not be applied clinically. Ideally, appliances should be tested in all three dimensions with techniques (e.g. type of ligation) that replicate clinical procedures. This can be accomplished with a new patented technology, the orthodontic force tester (OFT). The OFT allows an entire arch with brackets and a full arch wire to be set up while measurements are made on target teeth. With the OFT, appliances can be tested to ascertain if they provide the prescribed load system, and if not, then modify them or develop new ones. In this experiment two different commercially available prefabricated closing loop arch wires (keyhole and teardrop) were tested with variations in gable bends, interbracket loop position, and activation. The application being tested is closing space between a lateral incisor and canine in a first premolar extraction case after the canine has been retracted. While the trend shows that the keyhole loop produces higher overall force the two loops are not significantly different in the forces or moments that they generate. The one exception is that the keyhole loop produces higher lingual forces at the canine when the loop is in the mesial position. Also, few wire configuration were able to produce M/F sufficient to translate teeth. The wire configurations that can provide the proper load system to translate teeth in the lingual direction at the incisor were in the mesial position and had second order gable bends at the alpha position. The loop design had little effect on the M/F ratios.

Indiana University-Purdue University Indianapolis (IUPUI) / Orthodontic closing loops offer an efficient way to control the moment to force ratios (M/F) delivered during space closure. The triangular loop is often used in the Graduate Orthodontic Clinic at the Indiana University School of Dentistry. Previous studies on the triangular loop were concerned with various loop geometries. The present project was designed to study the triangular loop in a clinically realistic experimental set up. Compared to the previous studies, three major changes were implemented: instead of two coplanar brackets, the current study employed a bracketed typodont arch (1) the effects of loop locations (2) and different interbracket distances were considered (3). The measured moment and forces reflect considerable differences in the systems due to the new experimental set up. As in previous studies, the triangular loops were fabricated from 0.016 X 0.022- inch stainless steel wire. The loops were equilateral triangles with 8 mm sides, ligated to the arch wire by elastomeric rings. There were 4 loop locations: location 1 was at 1.2 mm away from the mesial bracket; location 2 was at 3 .2 mm away from the distal bracket; location 3 was centered in the middle of the original interbracket distance; location 4 was located 2.6 mm away from the mesial bracket. There were three interbracket distances (IB). The original IB (IBl) of 12.6 mm was decreased by 3 mm (IB 2) and by 6 mm (IB 3). The loops were activated by 1.6 mm and 3.3 mm. Force and moment components were measured along three mutual perpendicular axes (x, y, and z) corresponding to the buccolingual, mesiodistal, occlusogingival axes respectively. Comparisons of Mx/Fy and Mz/Fy at the mesial and distal, by three activation levels, three interbracket distances, and four locations, and all interaction effects, were performed using a mixed design repeated measures ANOV A procedure. The General Linear Model (GLM) procedure for unbalanced designs was used because not all interbracket distances could be accommodated with all loop locations. Activation distance was the within specimen repeated factor. Loop location and interbracket distance were the between specimen factor. It was theorized that the location of the triangular loop, as well as the interbracket distance, have a considerable effect on the generated M/F. The Null Hypothesis was that there are no significant differences (p > 0.05) in the M/F ratios generated by the triangular loop as the loop position changes relative to the brackets, and there are no significant differences (p>0.05) in the M/F ratios generated by the triangular loop as the interbracket distance becomes shorter with space closure. Statistical significant interactions were found for Mx/Fy and Mz/Fy at location 2, for all activations, at both the mesial and distal measures. Therefore we rejected the first part of the Null Hypothesis (no differences as the loop location changes), and accept the second part (no differences as the interbracket distance shortens). We were able to see clear trends at all loop locations, as well as interbracket distances, and draw useful clinical implications. We found that the mesial closing forces are quite small when compared to those at the distal. We attributed this discrepancy to the U shape geometry of the continuous arch wire technique. We observed that if closing loops are delivered with no activation, then counterproductive M/F ratios are produced. Our data also indicated that anchorage becomes more critical as the interbracket distance shortens. Finally, we determined that wire tie ligation for prevention of rotation along the long axis of the tooth is especially important for the lateral incisor.

During development of the nervous system, formation of specific connections between nerve cells depends on the stability of growing axons to reach appropriate target cells and form synapses. In culture, hippocampal neurons form numerous synapses by developing axonal and dendritic extensions. To elucidate principles of neuronal signaling and network establishment, creation of neuronal networks in which connectivity and pathways can be experimentally controlled is of great interest. In the present study we used a microcontact printing technique to control and study neurite outgrowth of hippocampal neurons in vitro. My preliminary results show that hippocampal neurons follow the microcontact printed pattern of poly-D-lysine (PDL). In doing so, neurons retain their morphology with normal subcellular distribution of various cell adhesion and synaptic molecules. However, the distribution of various axonal or dendrite components is altered. Hence we have developed a system in which isolated axons and dendrites align with inputs from very few neurons. With this technique we intend to study axon-dendrite communications on a spatially restricted and defined substrate.

No description available.

Os praguicidas carbamatos surgiram na década de 70. Este grupo de substâncias possui atividade anticolinesterásica, com variado grau de toxicidade. São solúveis em água e termicamente instáveis. O praguicida aldicarbe faz parte do grupo dos carbamatos, sendo um metil carbamato de oxima. Contaminações de águas subterrâneas e superficiais pelo aldicarbe já foram demonstradas, devido a alta solubilidade deste composto em água e sua alta capacidade de lixiviação em solo. Este trabalho visa o desenvolvimento de métodos analíticos para separação e quantificação de aldicarbe, aldicarbe sulfóxido e aldicarbe sulfona, por cromatografia líquida de alta eficiência, cromatografia líquida acoplada à espectrometria de massas e eletroforese capilar. O trabalho objetiva ainda avaliar a toxicidade aguda das três substâncias utilizando a bactéria marinha luminescente Vibrio fischeri. Amostras de água ultrapura, superficial e subterrânea foram submetidas a etapas de extração em diferentes cartuchos de fase sólida para avaliação da recuperação dos analitos e a realização da pré concentração dos mesmos. No método proposto por cromatografia líquida acoplada a espectrometria de massas, obteve-se limite de quantificação de 10 &#181;g.L-1, sendo que o alcançado no método proposto por cromatografia líquida com detecção por arranjo de diodos foi de 2 &#181;g.L-1. Já o método desenvolvido por eletroforese capilar com detecção por arranjo de diodos teve um limite de quantificação de 10 mg.L-1. Os resultados de CE50 obtida para o aldicarbe, aldicarbe sulfona e aldicarbe sulfóxido, no teste de toxicidade com a bactéria luminescente Vibrio fischeri foram respectivamente: 56,0 mg.L-1, 47,0 mg.L-1 e 7,8 mg.L-1. O método desenvolvido por cromatografia líquida se mostrou com sensibilidade satisfatória para análise de aldicarbe e seus produtos de transformação em água com níveis de quantificação dos compostos abaixo do limite determinado pela OMS (10 &#181;g.L-1. O método por eletroforese capilar não se mostrou com sensibilidade ideal para detecção dos analitos em níveis de traços. No teste de toxicidade aguda, observou-se que o aldicarbe sulfóxido é cerca de 7 vezes mais tóxico para a bactéria que o próprio aldicarbe, o que já é descrito na literatura para outras espécies. / Carbamates pesticides first appeared in the 1970s. This group of substances possesses anticholinesterasic activity, with varied toxicity degree. They are soluble in water and thermally unstable. The aldicarb pesticide belongs to the carbamates group, being an oxime methyl carbamate. Contaminations of underground and superficial waters by aldicarb have been demonstrated, due to the high solubility of this compound in water and its high capacity of lixiviation in soil. This dissertation aims to develop analytical methods for separation and quantification of aldicarb, aldicarb sulfoxide, and aldicarb sulfona, by high efficiency liquid chromatography, liquid chromatography-tandem mass spectrometry and capillary electrophoresis. The dissertation also focuses on evaluating the acute toxicity of the three substances using the luminescent marine bacterium Vibrio fischeri. Samples of ultrapure water, superficial and underground, were submitted to extraction stages in different cartridges of solid phase for evaluating the recovery of analytes and conducting their pre-concentration. In the method proposed by liquid chromatography-tandem mass spectrometry, a quantification limit of 10 &#181;g.L-1 was obtained, in comparison with the 2 &#181;g.L-1 achieved in the method proposed by liquid chromatography with diode-array detection. The method developed by capillary electrophoresis with diode-array detection had a quantification limit of 10 mg L-1. The results of CE50 obtained for the aldicarb, aldicarb sulfone and aldicarb sulfoxide, in the toxicity test with the luminescent bacterium Vibrio fischeri were respectively: 56.0 mg.L-1, 47.0 mg.L-1 e 7.8 mg.L-1 The method developed by liquid chromatography showed satisfactory sensitivity for analysis of aldicarb and its transformation products in water with quantification levels of the compounds below the limit determined by OMS (10 &#181;g.L-1</sup) . The method by capillary electrophoresis did not show ideal sensitivity for trace level detection of analytes. In the acute toxicity test, it was observed that the aldicarb sulfoxide is nearly 7 times more toxic for the bacterium than the aldicarb itself, which is already described in the literature for other species.