In recent years, wireline temperature profiling methods have evolved to offer new insight into fractured rock hydrogeology. An important advance in temperature logging makes use of boreholes temporarily sealed with flexible impervious fabric liners so that the water column is static and effects of cross-connection are eliminated. For this project a characterization technique was developed based on combining fibre optic distributed temperatures sensing (DTS) with active heating within boreholes sealed with underground liners. DTS systems provide a temperature profiling method that offers improved temporal resolution when compared with wireline trolling based techniques. The ability to collect temperature profiles rapidly in time can improve understanding of transient processes. In this study the advantage of a sealed borehole environment for temperature investigations is demonstrated. Evidence for identifying active groundwater flow under natural gradient conditions using DTS heat pulse testing is presented through a comparison with high resolution geologic logging and hydraulic datasets.