Made available in DSpace on 2017-07-21T14:19:27Z (GMT). No. of bitstreams: 1 Luiz Antonio Zanlorensi Junior.pdf: 2319566 bytes, checksum: 155395ad70f3e7f006f2804616464c42 (MD5) Previous issue date: 2016-02-22 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The culture of plant tissues is one of the most successful areas of biotechnology, having highlight in industrial and commercial plants for various applications, such as genetic improvement and also for plant physiology research and in vitro industrial production of secondary compounds. The micropropagation is a tissue culture technique, considered the greatest impact on agriculture due to the fact contribute to the development of healthy plants and higher quality. One of the main problems of conventional micropropagation process is spent on labor. One solution to this problem is the use of bioreactors for the production of plants in vitro, being the latest technology and the most promising alternative in this area. In this context, this study aimed to develop an integrated temporary immersion bioreactor system to a computer system. The system developed can be used for production in large scale and research. It was also shown part of a protocol, demonstrating that the amounts of culture medium between 175 ml and 180 have best results for the production of Blackberry Cv. Tupy in temporary immersion bioreactors systems. / A cultura de tecidos de plantas é uma das áreas de maior êxito da biotecnologia, possuindo destacada posição comercial e industrial de plantas em diversas aplicações, como melhoramento genético e também em pesquisas em fisiologia vegetal e produção industrial in vitro de compostos secundários. A micropropagação é uma técnica de cultura de tecido, considerada a de maior impacto na agricultura, devido ao fato de contribuir com o desenvolvimento de plantas sadias e com maior qualidade. Um dos principais problemas do processo convencional de micropropagação é o gasto com mão de obra. Uma solução para esse problema é o uso de biorreatores para a produção de plantas in vitro, sendo a tecnologia mais recente e a alternativa mais promissora nessa área. Nesse contexto, esse trabalho teve por objetivo desenvolver um sistema de biorreator de imersão temporária integrado a um sistema computacional. O sistema desenvolvido pode ser utilizado para produção em escala e para experimentos com fins de pesquisas. Também foi apresentado parte de um protocolo, demonstrando que as quantidades de meio de cultura entre 175 e 180 ml obtiveram melhores resultados para a produção de Amora Preta Cv. Tupy em sistemas de biorreatores de imersão temporária.

An efficient micropropagation protocol for large scale production of hazelnut plants is required for consistent supply of elite germplasm to support the growing industry in Ontario. The main focus of current research was to develop a bioreactor based micropropagation protocol for hazelnut multiplication. An integrated approach was developed to increase the multiplication rate by optimizing the nutrient medium supplements and culture technology using a temporary immersion bioreactor system (TIS). As compared to conventional semi-solid and liquid based culture system, the Liquid LabTM temporary immersion bioreactor system (LIS) showed a significant enhancement in the number of shoots (3.3 shoots/explant), shoot height, leaf area and chlorophyll content in micropropagated plantlets. Antioxidant supplements such as ascorbic acid and melatonin along with iron (460 µM FeEDDHA) significantly increased the shoot proliferation (5.5 shoots/explant). However, a much higher shoot proliferation (10.9 shoots/explant) was observed with the addition of aspirin (10 µM) in the presence of BA (17.6 µM). Among several hazelnut cultivars, HF-16 had the highest multiplication rate followed by Geneva and Epsilon. Medium supplemented with 10 µM IBA was the best for rooting of microshoots of HF-16 and the plantlets acclimatized in the green house with 80% survival rate. / Ontario Ministry of Agriculture and Food and Rural Affairs