This study examined professional identity of biology faculty at four-year colleges and universities and the relationship between their perceived identity, as a scientist or a teacher, and their participation in activities related to evidence-based teaching practices. This study drew upon online survey results of 328 college and university biology faculty from across the country. Results from ordinary least squares regression indicated faculty who work at very high, high, and Master's institutions had higher perceptions of science identity. Nontenure track faculty showed higher perceptions of teaching identity. Additionally, the results of this study indicated a strong teaching identity and favorable views on evidence-based teaching related to greater participation in evidence-based teaching practices. Science identity, though positively relate to the outcome, was not a statistically significant predictor of participation in activities related to evidence-based teaching practices. An implication for practice is to recognize biology faculty members' use of newer teaching methods, such as demonstrations of student-centered teaching in lectures in promotion and tenure review. An important implication for future research would be to explore if recognition for outstanding teaching through promotion and tenure cause an increase in the number of faculty who gravitate toward more education-based scholarly activities and, in turn, develop a higher teaching identity.