Context. During each iteration of the application, we need to test the usability of the application. Therefore, how to improve efficiency and test according to the user's real experience is a problem faced by the company. According to our preliminary research on the literature. In the past, the usability of software systems was evaluated subjectively and the process was not well defined [21]. Every usability evaluation method has its advantages and disadvantages. Some are difficult to apply, and others are dependent on the measurer's opinions or instruments [21]. Moreover, currently, there are few studies on usability testing for e-commerce applications. And there is a lack of a usability test optimization scheme suitable for e-commerce applications. Therefore, we choose this direction for research. Objectives. This paper aims to provide an optimization method for usability testing process based on user requirements for e-commerce applications existing in the market, so that enterprises can more effectively implement the usability testing process in the process of iterative update of applications. Methods. In order to find the answers to the research questions, we used Literature review and Survey to collect the data. In the Survey section, we used the Questionnaire and Interview methods. After the data collection is completed, we perform quantitative and qualitative analysis of the data and organize the research conclusions. Results. Through a literature review, we conducted a systematic review of the usability challenges in e-commerce applications and how to overcome them. And we found the research direction in this process. We use the questionnaire to obtain the usability requirements of respondents (also users of e-commerce applications) for e-commerce applications, and use our optimization method (EEC) to translate user's requirements into usability test script content. And according to the feedback of the respondents to determine the content priority. We used interviews to understand the priorities that companies follow when actually performing usability testing. And compare it with the priority of our script to verify the effectiveness of our method. Conclusions. We summarize relevant conclusions by finding answers to research questions. Finally, an optimization method for usability testing is summarized, which will help future testers better implement usability testing to a certain extent.