The purpose of the research is to construct an instruction program about integrating Big6 skills approach with web-based theme learning by using systematic instruction design model to analyze learners¡¦ background, the content of the curriculum, instruction goals and evaluation methods. The researcher designed teaching activities and learning materials based on two topics in science curriculum¡Xclimate and constellation, and furthermore took the teaching action research divided into two sections and three cycles. Through feedback, reflection and correction, the instruction activities in this program has been developed and divided into four sections¡X(1) to lead to theme learning, (2) Big6 research plan, (3) group learning, (4) result exhibition and evaluation. The instruction program was put into practice in one fifth-grade class of elementary school. After the action research, the students with different abilities showed different learning effect. The result that over 90% students said they gained satisfied achievement and over 70% passed the cognitive test in Big6 skills revealed most of the students really learned the content of the instruction program, but a few students needed the teacher and peer groups to provide much more scaffoldings and learning opportunities, especially in defining questions and finding information. After the three- cycle instruction program, the students got the learning abilities as follows-- (1) learning Bid6 systematic thinking model, (2) learning Big6 skill research method, (3) learning how to write theme reports, (4) learning how to present their products, (5) learning evaluation and reflection, (6) improving abilities to apply technology, (7) learning knowledge related to topics, and revealing the self-leading learning style under the influence of Big6 instruction. The web-based instruction environment constructed on ¡§Open network education platform system like Educities¡¨ can reduce the teacher¡¦s pressure on managing web-based instruction. The functions can cope with the need of the web-enhanced instruction, and become the important communication media between teacher and students in students¡¦ active learning section. The characteristic of the internet on multiple communication mechanism without limitation of space and time can expand the effect on active learning.