The Intermountain West is comprised of impressive land formations, numerous ecoregions, and a unique biota. The area has many flora and fauna that have been investigated, but the region is generally considered undersampled when it comes to insects. However, I propose the matter to be a lack of shared experience in identifying key insect species and the underutilization of professional and personal collections. These impediments are highlighted by two insect groups in the Intermountain West: spongillaflies and tiger moths.Spongillaflies can be difficult to recognize for the general entomologist and have rarely been recorded in the Intermountain West. My colleagues and I recently discovered a large population of spongillaflies in Utah that we present as a substantial additional record. I also followed the population throughout the 2016 field season to make natural history observations. I identified the spongillaflies to be Climacia californica and their associated host to be Ephydatia fluviatilis. During the season, a total of 1,731 specimens were collected, light traps were the most effective sampling technique and the population had one mass emergence event. I hope my work and figures will help investigators as they continue to search the area for spongillaflies. Tiger moths on the other hand have largely been collected in the Intermountain West and are easily recognized, generally being brightly colored. Because of these bright colors, they attract collectors and have been sampled heavily throughout the Intermountain West. However, until now, these records have not been utilized and tucked away in collections. We took the vast amount of records and used them to create predicted models of biogeography for each tiger moth species in the area. We successfully created species level ecological niche models (ENM) analyzing environmental variables such as temperature, precipitation, elevation, and vegetation. Overall, I found tiger moths can be collected almost everywhere and during each month of the year with 93 different species scattered across the region. I anticipate our ENM models to help researchers locate tiger moths of interest to investigate within the Intermountain West.During my studies, I investigated in detail the lichen feeding tiger moths (Lithosiini). Many tiger moths eat toxic plants, but only a few in the area consume lichen, an unusual host because of their secondary defensive chemicals. I investigated how these chemicals impacted Cisthene angelus caterpillars host selection by simultaneously offering them various lichens with differing chemistries. I expected these caterpillars to avoid usnic acid as it deterred other lichen feeding tiger moths. However, these caterpillars surprised me by consistently consuming the acid and being largely polyphagous. Our future work will be directed at how caterpillars balance nutritional needs and the chemicals they sequester.

The Intermountain West is comprised of impressive land formations, numerous ecoregions, and a unique biota. The area has many flora and fauna that have been investigated, but the region is generally considered undersampled when it comes to insects. However, I propose the matter to be a lack of shared experience in identifying key insect species and the underutilization of professional and personal collections. These impediments are highlighted by two insect groups in the Intermountain West: spongillaflies and tiger moths. Spongillaflies can be difficult to recognize for the general entomologist and have rarely been recorded in the Intermountain West. My colleagues and I recently discovered a large population of spongillaflies in Utah that we present as a substantial additional record. I also followed the population throughout the 2016 field season to make natural history observations. I identified the spongillaflies to be Climacia californica and their associated host to be Ephydatia fluviatilis. During the season, a total of 1,731 specimens were collected, light traps were the most effective sampling technique and the population had one mass emergence event. I hope my work and figures will help investigators as they continue to search the area for spongillaflies. Tiger moths on the other hand have largely been collected in the Intermountain West and are easily recognized, generally being brightly colored. Because of these bright colors, they attract collectors and have been sampled heavily throughout the Intermountain West. However, until now, these records have not been utilized and tucked away in collections. We took the vast amount of records and used them to create predicted models of biogeography for each tiger moth species in the area. We successfully created species level ecological niche models (ENM) analyzing environmental variables such as temperature, precipitation, elevation, and vegetation. Overall, I found tiger moths can be collected almost everywhere and during each month of the year with 93 different species scattered across the region. I anticipate our ENM models to help researchers locate tiger moths of interest to investigate within the Intermountain West. During my studies, I investigated in detail the lichen feeding tiger moths (Lithosiini). Many tiger moths eat toxic plants, but only a few in the area consume lichen, an unusual host because of their secondary defensive chemicals. I investigated how these chemicals impacted Cisthene angelus caterpillars host selection by simultaneously offering them various lichens with differing chemistries. I expected these caterpillars to avoid usnic acid as it deterred other lichen feeding tiger moths. However, these caterpillars surprised me by consistently consuming the acid and being largely polyphagous. Our future work will be directed at how caterpillars balance nutritional needs and the chemicals they sequester.

<p dir="ltr">The lichen-feeding tiger moth tribe Lithosiini (Erebidae: Arctiinae) represent the largest radiation of invertebrate lichenivory. Caterpillars feed on lichen and as they feed, also sequester lichen polyphenolics, a behavior unique to these insects. The role of these compounds is believed to defend lithosiines against predators as larvae have been found to be protected against predators such as ants and moths to predators such as birds and bats. Experimental testing with controlled diets is necessary to fully make this connection, however little is known about host specifics for lithosiines. Furthermore, although lithosiines are monophyletic, the lack of a fully resolved phylogeny hampers investigation into many of the shallower level relationships, e.g. those among genera and species, within the group.</p><p dir="ltr">I addressed these knowledge gaps using the subtribe Cisthenina. Members of this group have been used to investigate predator-prey interactions and been included in morphological and molecular studies. Thus, while the group still needs attention, there is an ample amount of legacy loci data available for its members. I used these data to investigate the evolutionary relationships at the genus level, but to increase resolution in my analyses I additionally sampled taxa throughout the group with a recently developed anchored hybrid enrichment (AHE) probe set. I combined it with the legacy loci to both increase taxon sampling and resolution. I confirmed that trees made strictly from the legacy loci were unsuccessful and resulted in poorly supported relationships that made little sense. The addition of the AHE data greatly helped resolve relationships, however, there remained areas that were poorly supported and they appear to be genera with only a few loci. Thus, there is still room for improvement, but this offers a way for moving forward in lithosiine research, particularly to involve others who may have limited funding, equipment, and/or personnel and may only be able to afford legacy loci in diverse collaborations.</p><p dir="ltr">As the AHE probe set worked well with genus-level relationships I further attempted to use it in species delimitation of the notorious <i>Hypoprepia fucosa</i>-<i>miniata </i>species complex. Members of this group are varying shades of yellows, oranges and reds and have a convoluted taxonomic history. I gathered and organized over 4,000 specimens and using the AHE probe set found support for five distinct species. Interestingly, I used other morphological characters such as genitalia, but found no differences between species and a large amount of intraspecific variation. This suggests other courtship behaviors may be present and external morphology, i.e., color patterns, remain the best way to identify species. As part of this I am describing a new species and raising one from subspecies and as species are now readily distinguishable, they can be used for further investigations into lithosiines.</p><p dir="ltr">I used a member of this complex, <i>H</i>. <i>fucosa</i> to then evaluate the use of species distribution models (SDMs) to better understand their niche and how it relates to plausible lichen hosts. I evaluated 17 lichen species from two lichen genera, <i>Physcia </i>(13 species) and <i>Myelochro</i><i>a </i>(4 species)<i>. </i>These genera were selected based on previous feeding assays and the metabolites found in them have also been found in <i>H</i>. <i>fucosa </i>further suggesting caterpillars may feed on them. SDMs typically only use environmental factors to define and predict species niches. I compared the niches described by traditional SDMs to assess how similar they were, but I also investigated the use of lichens as biotic factors in the models. I assessed the influence each lichen had on the moth’s distribution found the niche of every lichen to be significantly different than that of the moth and their inclusion in SDMs of <i>H</i>. <i>fucosa </i>to improve model performance. This suggests <i>H</i>. <i>fucosa </i>caterpillars to be polyphagous, but to have some connection with these lichens. Further investigation with live specimens is needed, but these results support this as an effective way to describe lithosiine niches to better understand lichen feeding.</p>